ID H3CIN2_TETNG Unreviewed; 975 AA.
AC H3CIN2;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
OS Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon
OS nigroviridis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Tetraodon.
OX NCBI_TaxID=99883 {ECO:0000313|Ensembl:ENSTNIP00000008110.1, ECO:0000313|Proteomes:UP000007303};
RN [1] {ECO:0000313|Proteomes:UP000007303}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15496914; DOI=10.1038/nature03025;
RA Jaillon O., Aury J.-M., Brunet F., Petit J.-L., Stange-Thomann N.,
RA Mauceli E., Bouneau L., Fischer C., Ozouf-Costaz C., Bernot A., Nicaud S.,
RA Jaffe D., Fisher S., Lutfalla G., Dossat C., Segurens B., Dasilva C.,
RA Salanoubat M., Levy M., Boudet N., Castellano S., Anthouard V., Jubin C.,
RA Castelli V., Katinka M., Vacherie B., Biemont C., Skalli Z., Cattolico L.,
RA Poulain J., De Berardinis V., Cruaud C., Duprat S., Brottier P.,
RA Coutanceau J.-P., Gouzy J., Parra G., Lardier G., Chapple C.,
RA McKernan K.J., McEwan P., Bosak S., Kellis M., Volff J.-N., Guigo R.,
RA Zody M.C., Mesirov J., Lindblad-Toh K., Birren B., Nusbaum C., Kahn D.,
RA Robinson-Rechavi M., Laudet V., Schachter V., Quetier F., Saurin W.,
RA Scarpelli C., Wincker P., Lander E.S., Weissenbach J., Roest Crollius H.;
RT "Genome duplication in the teleost fish Tetraodon nigroviridis reveals the
RT early vertebrate proto-karyotype.";
RL Nature 431:946-957(2004).
RN [2] {ECO:0000313|Ensembl:ENSTNIP00000008110.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; H3CIN2; -.
DR STRING; 99883.ENSTNIP00000008110; -.
DR Ensembl; ENSTNIT00000008274.1; ENSTNIP00000008110.1; ENSTNIG00000005422.1.
DR GeneTree; ENSGT00550000074910; -.
DR HOGENOM; CLU_001493_7_2_1; -.
DR InParanoid; H3CIN2; -.
DR OMA; HCWRCKT; -.
DR TreeFam; TF300518; -.
DR Proteomes; UP000007303; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000007303}.
FT DOMAIN 59..674
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 719..832
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 975 AA; 108401 MW; B8711B8FEA4EE51B CRC64;
RAFSTEEGCV QPAEVGSAQS LYRDTVLVPR TDFPMKVTGQ KLLDQEVAIQ KKCGFADLYA
WQRSRKAKKE FCVHDGPPYA NGDPHVGHAL NKILKDIRNR FEMLKGRRVH YIPGWDCHGL
PIELKALGEL TPSSLTPLQI RQKARAFAEE AITRQKAAFQ RWGVMADWDH CYYTHHGVYE
AAQLKVFQEM HGKGFIYQDH KPVFWSPSSR TALAEAELEY NPDHVSRAIY ATFPLTTVPA
NIASEVAGLD NVSVLVWTTQ PWTIPANQAV CYMPKAQYSV VKRTDSSQML LIAAERTASL
ASLLGTELET VGTFTEGSQL EGGICKHPVI PDKFVQLLPA NHVTMAKGTG LVHTAPAHGM
DDYSVASHFN LPVECMVDED GKFTELAGPE LHNLAVMTEG TDKAPTVISM LKDCGALVNE
ERCVHSYPYD WRTKQPVIIR PSRQWFIDTA SLKDKAKEAL KKVHILPESA RGSLLAMLDR
RTYWCISRQR SWGVPIPVFY HRETGEPLLN KYTMSHITSL FKEKGSDCWW ELPLETLLPE
EVLEKSKAGP AIDYVRGEDV LDIWFDSGTS WAAVLEETGC MADAYLEGKD QIGGWFQSSL
LTSVAVRNKA PYRSLVVHGF VVGEKGEKMS KSVGNVVDPD EVINGGKDTA TTAYGADVLR
WWVAESNVFS EVQIGANVLN SARDDINKLR NSLRFLLGNL QGFDPQVQAT EPADMHYIDQ
YMLHLLHKYS IKVTDAYSEF GGRVVRVLQA FITGDLSSFY FSIIKDRLYC EPEDSLGRRS
CQTVLEKVLD GVTRSIAPIL PHLAEEVYAH SPGHKETQTL FKSGWIQSTS VWLRPGVEEM
VEGACAIRDS FLSSIPGKNP AKYDLTIAIK PGLLFELMEC LQGEATSTSS QLAELMMVAR
VDLTSELPVD LPPDALLSHG TFLINLEGGV IRENSAYAIA AVPTSGSRCP RCRRHTAETA
DALCPRCRTV LQSSQ
//
