ID H3NSN4_9GAMM Unreviewed; 456 AA.
AC H3NSN4;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE SubName: Full=Alkaline phosphatase {ECO:0000313|EMBL:EHQ57097.1};
GN ORFNames=OMB55_00008180 {ECO:0000313|EMBL:EHQ57097.1};
OS gamma proteobacterium HIMB55.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales; Halieaceae.
OX NCBI_TaxID=745014 {ECO:0000313|EMBL:EHQ57097.1, ECO:0000313|Proteomes:UP000003408};
RN [1] {ECO:0000313|EMBL:EHQ57097.1, ECO:0000313|Proteomes:UP000003408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HIMB55 {ECO:0000313|EMBL:EHQ57097.1,
RC ECO:0000313|Proteomes:UP000003408};
RX PubMed=22493201; DOI=10.1128/JB.00171-12;
RA Huggett M.J., Rappe M.S.;
RT "Genome Sequence of Strain HIMB55, a Novel Marine Gammaproteobacterium of
RT the OM60/NOR5 Clade.";
RL J. Bacteriol. 194:2393-2394(2012).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 1 Mg(2+) ion. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR601952-2};
CC Note=Binds 2 Zn(2+) ions. {ECO:0000256|PIRSR:PIRSR601952-2};
CC -!- SIMILARITY: Belongs to the alkaline phosphatase family.
CC {ECO:0000256|RuleBase:RU003946}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHQ57097.1}.
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DR EMBL; AGIF02000001; EHQ57097.1; -; Genomic_DNA.
DR AlphaFoldDB; H3NSN4; -.
DR STRING; 745014.OMB55_00008180; -.
DR PATRIC; fig|745014.4.peg.835; -.
DR eggNOG; COG1785; Bacteria.
DR OrthoDB; 9794455at2; -.
DR Proteomes; UP000003408; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016791; F:phosphatase activity; IEA:InterPro.
DR CDD; cd16012; ALP; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 1.
DR InterPro; IPR001952; Alkaline_phosphatase.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR PANTHER; PTHR11596; ALKALINE PHOSPHATASE; 1.
DR PANTHER; PTHR11596:SF5; ALKALINE PHOSPHATASE; 1.
DR Pfam; PF00245; Alk_phosphatase; 1.
DR PRINTS; PR00113; ALKPHPHTASE.
DR SMART; SM00098; alkPPc; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR601952-3};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601952-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601952-2};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000003408};
KW Signal {ECO:0000256|SAM:SignalP}; Zinc {ECO:0000256|PIRSR:PIRSR601952-2}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..456
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003591025"
FT ACT_SITE 84
FT /note="Phosphoserine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-1"
FT BINDING 35
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 35
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 135
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 137
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 321
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 326
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 330
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 368
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 369
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT BINDING 420
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-2"
FT DISULFID 150..169
FT /evidence="ECO:0000256|PIRSR:PIRSR601952-3"
SQ SEQUENCE 456 AA; 48491 MW; 7F9CA417D5B1D122 CRC64;
MRFFNAPTLL TTLLGIFSAQ SAYADPKSVI LIIGDGFDDV HVTMGRNYLS GQGGTLTLDT
MPFRGAVQVE TVDDNGTPIY VADSANTATT LATGSVTRIG RVGKDRDDES VTTVLEAAAA
AGYKTGIVST ASVTDATPAA FAAHVGIRAC ENPVTIRGGE KYGVTFPGCP KDLEENGGAG
SISEQLATAD VDVILGGGME HFAAQYETQP TALDKATESG ATVLTSAAQL NANYSGRVIG
LFSEDTMPVQ WQGSDGRSAE DIERSWLNLA HEMIGSITPP ETISCVDNPA FEDMPSLNAM
TRFALRHLDK ENERGFFLTI ESASIDKKSH VRDPCGSIGE IAQLEQALAA ALEHAANNPD
TLIIVTADHA QAAQIVPEPT LYTGLPIPVF SPGKVARVET PEGSVMRINY ATNNIQSEEH
TGANVPLFAN AEGNKVLSPF MRQREIYQAM MQYLEL
//
