ID   H4GK00_9LACO            Unreviewed;       435 AA.
AC   H4GK00;
DT   18-APR-2012, integrated into UniProtKB/TrEMBL.
DT   18-APR-2012, sequence version 1.
DT   24-JAN-2024, entry version 51.
DE   RecName: Full=Glutamate decarboxylase {ECO:0000256|ARBA:ARBA00012421, ECO:0000256|RuleBase:RU361171};
DE            EC=4.1.1.15 {ECO:0000256|ARBA:ARBA00012421, ECO:0000256|RuleBase:RU361171};
GN   ORFNames=PS3_14606 {ECO:0000313|EMBL:EHS85882.1};
OS   Limosilactobacillus gastricus PS3.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Limosilactobacillus.
OX   NCBI_TaxID=1144300 {ECO:0000313|EMBL:EHS85882.1, ECO:0000313|Proteomes:UP000004567};
RN   [1] {ECO:0000313|EMBL:EHS85882.1, ECO:0000313|Proteomes:UP000004567}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PS3 {ECO:0000313|EMBL:EHS85882.1,
RC   ECO:0000313|Proteomes:UP000004567};
RX   PubMed=23846278;
RA   Martin V., Cardenas N., Jimenez E., Maldonado A., Rodriguez J.M.,
RA   Fernandez L.;
RT   "Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from
RT   Human Milk.";
RL   Genome Announc. 1:E00489-E00413(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + L-glutamate = 4-aminobutanoate + CO2;
CC         Xref=Rhea:RHEA:17785, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:59888; EC=4.1.1.15;
CC         Evidence={ECO:0000256|ARBA:ARBA00000018,
CC         ECO:0000256|RuleBase:RU361171};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933,
CC         ECO:0000256|PIRSR:PIRSR602129-50, ECO:0000256|RuleBase:RU000382};
CC   -!- SIMILARITY: Belongs to the group II decarboxylase family.
CC       {ECO:0000256|RuleBase:RU000382}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EHS85882.1}.
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DR   EMBL; AICN01000052; EHS85882.1; -; Genomic_DNA.
DR   AlphaFoldDB; H4GK00; -.
DR   STRING; 1144300.PS3_14606; -.
DR   PATRIC; fig|1144300.3.peg.1217; -.
DR   Proteomes; UP000004567; Unassembled WGS sequence.
DR   GO; GO:0004058; F:aromatic-L-amino-acid decarboxylase activity; IEA:UniProt.
DR   GO; GO:0004351; F:glutamate decarboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0006536; P:glutamate metabolic process; IEA:InterPro.
DR   CDD; cd06450; DOPA_deC_like; 1.
DR   Gene3D; 3.90.1150.160; -; 1.
DR   Gene3D; 4.10.280.50; -; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   InterPro; IPR010107; Glutamate_decarboxylase.
DR   InterPro; IPR002129; PyrdxlP-dep_de-COase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   NCBIfam; TIGR01788; Glu-decarb-GAD; 1.
DR   PANTHER; PTHR43321; GLUTAMATE DECARBOXYLASE; 1.
DR   PANTHER; PTHR43321:SF3; GLUTAMATE DECARBOXYLASE; 1.
DR   Pfam; PF00282; Pyridoxal_deC; 1.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE   3: Inferred from homology;
KW   Decarboxylase {ECO:0000256|RuleBase:RU361171};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|RuleBase:RU000382};
KW   Pyridoxal phosphate {ECO:0000256|PIRSR:PIRSR602129-50,
KW   ECO:0000256|RuleBase:RU000382};
KW   Reference proteome {ECO:0000313|Proteomes:UP000004567}.
FT   MOD_RES         246
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR602129-50"
SQ   SEQUENCE   435 AA;  50270 MW;  ED3F32B7419A966D CRC64;
     MNQQPTEPRI AYQVIKDQLL DEGNARQNLA TFCQTYMEPE ATKLMSETLE KNAIDKSEYP
     RTAEIENRCV NIIANLWHVQ SNEEYMGTST VGSSEACMLG GMAMKFAWRN RAKKLGLDLN
     AHKPNLVISS GYQVCWEKFC VYWDVELRTV PMDLNHQSLN MEKVMDYVDE YTIGIVGIMG
     ITYTGRYDDL EKLNDLVENY NQKTDYKVYI HVDAASGGLY APFMEPDLKW DFQLKNVVSI
     NTSGHKYGLV YPGIGWVLWR DQAYLPKELI FNVSYLGGEM PTMAINFSRS ASHIIGQYYN
     FIRYGFKGYH DIHQRTHDVA VYLAKEIEKL DLFEIVNDGS RLPIVCYRNK DNREHEWTLY
     DLADRLAMKG WQVPAYPLPA DLDQIEVQRI VVRADFGMGM AHDFIEDLKQ AIHDLDQAHI
     VFHEKTALKK YGFTH
//