ID H5U058_9ACTN Unreviewed; 1181 AA.
AC H5U058;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 24-JAN-2024, entry version 45.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=dnaE {ECO:0000313|EMBL:GAB39116.1};
GN ORFNames=GOSPT_058_00690 {ECO:0000313|EMBL:GAB39116.1};
OS Gordonia sputi NBRC 100414.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC Gordonia.
OX NCBI_TaxID=1089453 {ECO:0000313|EMBL:GAB39116.1, ECO:0000313|Proteomes:UP000005845};
RN [1] {ECO:0000313|EMBL:GAB39116.1, ECO:0000313|Proteomes:UP000005845}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 100414 {ECO:0000313|EMBL:GAB39116.1,
RC ECO:0000313|Proteomes:UP000005845};
RA Yoshida I., Hosoyama A., Tsuchikane K., Katsumata H., Yamazaki S.,
RA Fujita N.;
RT "Whole genome shotgun sequence of Gordonia sputi NBRC 100414.";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAB39116.1}.
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DR EMBL; BAFC01000058; GAB39116.1; -; Genomic_DNA.
DR AlphaFoldDB; H5U058; -.
DR eggNOG; COG0587; Bacteria.
DR Proteomes; UP000005845; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..73
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1181 AA; 129126 MW; 68BE37AF233949F2 CRC64;
MAGSFVHLHN HTEYSMLDGA AKVSPLFAEA KRLGMTAIGM TDHGNMFGAS EFYNTAIKNE
IKPIIGIEAY IAPESRFNTK RIQWGTRDQK GDDVSGSGAY THMTMVAENA TGLRNLFKLS
SLASIEGQLG KWARMDADLI AEYAEGIIAT TGCPSGEVQT RLRLGHDREA LEAAAKWQEI
FGKENFYLEL MEHGLEIERR VRDGLLDIGR TLGIKPIVTN DCHYVTKDHA ASHEALLCVQ
TGKTLTDPTR FKFDGDGYYL KSADEMRAQW DSVIPGACDN TLEIGERVQS YADVFTHRDR
MPIFPVPDGE SQQTWLRKEV ANGLAERRFP GVEVPEDYLR RANYELDVII QMGFPAYFLV
VGDLIRHAHE VGIRVGPGRG SAAGSLVAWA LGITNIDPIP HGLLFERFLN PERVSMPDID
IDFDDRRRGE MVRYATDRWG SDKVAQVITF GTIKTKAAIK DSARVQFGQP GFAIADKITK
ALPPPIMAKD IPVWGITNPE HERFGEATEV RTLIETDPDV KKIYDTAIGL EGLIRNAGVH
ACAVIMSSEP LTDAIPVWKR AQDGAIITGW DYPSCEAIGL LKMDFLGLRN LTVIGDALEN
IKLNRGIDLD LDTLPLEDTK TYELLAKGDT LGVFQLDGAA MRELLKMMQP TGFEDIVAVL
ALYRPGPMGV NAHTDYAKRK NGLQQIKPIH PELEEPLKEI LAETYGLIVY QEQIMQIAQK
VAGYSLGQAD LLRRAMGKKK KEILDEAYDG FAEGMRNNGF SQAAITALWD TVLPFAGYAF
NKSHAAGYGL VSFWTAYLKA NYPAEYMAGL LTSVGDDKDK AAIYLADCRK LGITVLPPDV
NESQHNFAAV GTDIRFGLAA IRNVGSGVVS SILAARDEKG KFTSFSDYLN KIDVTACNKK
VTESLVKAGA FDSLDHPRKG LFLIHVEAVE SVIGTKKAEA IGQFDLFGDA GGGADDTMAE
VFAVKVPDQE WDSKHKLALE REMLGLYVSG HPLSGVEHAI AAQTDTSITQ LLEGNISDGA
QITIGGIISS VTRRVNKKGE PWAAVTLEDM VGGVEVYFFP RAFQTYGMDI VADNIVLVKA
RVNMRDDSMM ISANDLAVPD LNAAGATKPV ALTLAARACT PDRVQALKQV LTRHPGPADV
HVTLVSDERS TVLKLTESLR VTPSSALMGD LKALLGPTCL G
//
