ID   H5YGQ8_9BRAD            Unreviewed;       443 AA.
AC   H5YGQ8;
DT   18-APR-2012, integrated into UniProtKB/TrEMBL.
DT   18-APR-2012, sequence version 1.
DT   27-MAR-2024, entry version 41.
DE   RecName: Full=Phenylacetate-coenzyme A ligase {ECO:0000256|PIRNR:PIRNR006444};
DE            EC=6.2.1.30 {ECO:0000256|PIRNR:PIRNR006444};
DE   AltName: Full=Phenylacetyl-CoA ligase {ECO:0000256|PIRNR:PIRNR006444};
GN   ORFNames=Bra471DRAFT_02810 {ECO:0000313|EMBL:EHR02063.1};
OS   Bradyrhizobium sp. WSM471.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Nitrobacteraceae; Bradyrhizobium.
OX   NCBI_TaxID=319017 {ECO:0000313|EMBL:EHR02063.1};
RN   [1] {ECO:0000313|EMBL:EHR02063.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM471 {ECO:0000313|EMBL:EHR02063.1};
RG   US DOE Joint Genome Institute;
RA   Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA   Peters L., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA   Kyrpides N., Ivanova N., Pagani I., Brau L., Yates R., O'Hara G., Rui T.,
RA   Howieson J., Reeve W., Woyke T.;
RT   "The Noncontiguous Finished sequence of Bradyrhizobium sp. WSM471.";
RL   Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the activation of phenylacetic acid (PA) to
CC       phenylacetyl-CoA (PA-CoA). {ECO:0000256|PIRNR:PIRNR006444}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-phenylacetate + ATP + CoA = AMP + diphosphate +
CC         phenylacetyl-CoA; Xref=Rhea:RHEA:20956, ChEBI:CHEBI:18401,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57390, ChEBI:CHEBI:456215; EC=6.2.1.30;
CC         Evidence={ECO:0000256|PIRNR:PIRNR006444};
CC   -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC       {ECO:0000256|PIRNR:PIRNR006444}.
CC   -!- SIMILARITY: Belongs to the phenylacetyl-CoA ligase family.
CC       {ECO:0000256|PIRNR:PIRNR006444}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM001442; EHR02063.1; -; Genomic_DNA.
DR   RefSeq; WP_007607919.1; NZ_CM001442.1.
DR   AlphaFoldDB; H5YGQ8; -.
DR   HOGENOM; CLU_035301_1_1_5; -.
DR   OrthoDB; 580775at2; -.
DR   UniPathway; UPA00930; -.
DR   Proteomes; UP000005774; Chromosome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0047475; F:phenylacetate-CoA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0010124; P:phenylacetate catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd05913; PaaK; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR028154; AMP-dep_Lig_C.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   InterPro; IPR049623; PA_CoA_lig_proteobact_actino.
DR   InterPro; IPR011880; PA_CoA_ligase.
DR   NCBIfam; TIGR02155; PA_CoA_ligase; 1.
DR   PANTHER; PTHR43439; PHENYLACETATE-COENZYME A LIGASE; 1.
DR   PANTHER; PTHR43439:SF1; PHENYLACETATE-COENZYME A LIGASE; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF14535; AMP-binding_C_2; 1.
DR   PIRSF; PIRSF006444; PaaK; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
PE   3: Inferred from homology;
KW   Ligase {ECO:0000256|PIRNR:PIRNR006444, ECO:0000313|EMBL:EHR02063.1};
KW   Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006444}.
FT   DOMAIN          94..299
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
FT   DOMAIN          346..440
FT                   /note="AMP-dependent ligase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF14535"
SQ   SEQUENCE   443 AA;  49584 MW;  EB4F1E154A504FBA CRC64;
     MALSRLKEGG NTYRAEMDAH ERASRDEIMA LQAQRLAWSL KHAYDNVPHY RKAFDAAGVH
     PSDFRELSDL AKFPFTVKTD LRDNYPFNMF AVPREKLVRV HASSGTTGKP IVVGYTQRDI
     DTWSDVMARS IRAAGGRTGM IIHNAYGYGL FTGGLGVHYG AEKLGCTVVP ISGGMTERQV
     QLINDFRPDI ITVTPSYMLA ILDEFKRQKL DPRQCSLKVG IFGAEPWTNA MRGEIEDAFD
     MDATDIYGLS EVIGPGVAQE CIETKDGLHI WEDHFYPEVI DPDTGVVLPD GEKGELVFTS
     LTKEAFPVIR YRTRDLTRLL PGTARPGMRR MEKVTGRSDD MIILRGVNLF PTQIEEVLLA
     TDWCGGHFIL ELTREGRMDE LTIIAEARPE SWDGRGLLDH ADRISTHVKN TIGISSNVRV
     VAPATLERSL GKAKRLYDKR PKD
//