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Database: UniProt
Entry: H6LC27
LinkDB: H6LC27
Original site: H6LC27 
ID   RNFB_ACEWD              Reviewed;         333 AA.
AC   H6LC27; C4N8U5;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2012, sequence version 1.
DT   16-JAN-2019, entry version 42.
DE   RecName: Full=Na(+)-translocating ferredoxin:NAD(+) oxidoreductase complex subunit B {ECO:0000305};
DE            EC=7.2.1.2 {ECO:0000269|PubMed:20921383, ECO:0000269|PubMed:24045950};
DE   AltName: Full=Rnf electron transport complex subunit B {ECO:0000255|HAMAP-Rule:MF_00463, ECO:0000305};
GN   Name=rnfB {ECO:0000255|HAMAP-Rule:MF_00463,
GN   ECO:0000303|PubMed:19222539};
GN   OrderedLocusNames=Awo_c22010 {ECO:0000312|EMBL:AFA48975.1};
OS   Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC
OS   1655 / WB1).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Eubacteriaceae;
OC   Acetobacterium.
OX   NCBI_TaxID=931626;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RX   PubMed=19222539; DOI=10.1111/j.1462-2920.2009.01871.x;
RA   Biegel E., Schmidt S., Muller V.;
RT   "Genetic, immunological and biochemical evidence for a Rnf complex in
RT   the acetogen Acetobacterium woodii.";
RL   Environ. Microbiol. 11:1438-1443(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RA   Poehlein A., Schmidt S., Kaster A.-K., Goenrich M., Vollmers J.,
RA   Thuermer A., Gottschalk G., Thauer R.K., Daniel R., Mueller V.;
RT   "Complete genome sequence of Acetobacterium woodii.";
RL   Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND SUBUNIT.
RC   STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RX   PubMed=20921383; DOI=10.1073/pnas.1010318107;
RA   Biegel E., Mueller V.;
RT   "Bacterial Na+-translocating ferredoxin:NAD+ oxidoreductase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:18138-18142(2010).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1;
RX   PubMed=24045950; DOI=10.1074/jbc.M113.510255;
RA   Hess V., Schuchmann K., Mueller V.;
RT   "The ferredoxin:NAD+ oxidoreductase (Rnf) from the acetogen
RT   Acetobacterium woodii requires Na+ and is reversibly coupled to the
RT   membrane potential.";
RL   J. Biol. Chem. 288:31496-31502(2013).
CC   -!- FUNCTION: Part of a membrane-bound complex that couples electron
CC       transfer with translocation of ions across the membrane. Couples
CC       electron transfer from reduced ferredoxin to NAD(+) with
CC       electrogenic movement of Na(+) out of the cell. Involved in
CC       caffeate respiration. {ECO:0000269|PubMed:20921383,
CC       ECO:0000269|PubMed:24045950}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + Na(+)(in) + NAD(+) + 2 reduced [2Fe-2S]-
CC         [ferredoxin] = Na(+)(out) + NADH + 2 oxidized [2Fe-2S]-
CC         [ferredoxin]; Xref=Rhea:RHEA:46800, Rhea:RHEA-COMP:10000,
CC         Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=7.2.1.2;
CC         Evidence={ECO:0000269|PubMed:20921383,
CC         ECO:0000269|PubMed:24045950};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00463};
CC       Note=Binds 7 [4Fe-4S] clusters. {ECO:0000305};
CC   -!- SUBUNIT: The complex is composed of six subunits: RnfA, RnfB,
CC       RnfC, RnfD, RnfE and RnfG. {ECO:0000255|HAMAP-Rule:MF_00463,
CC       ECO:0000305|PubMed:20921383}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00463}.
CC   -!- SIMILARITY: Belongs to the 4Fe4S bacterial-type ferredoxin family.
CC       RnfB subfamily. {ECO:0000255|HAMAP-Rule:MF_00463}.
DR   EMBL; FJ416148; ACR23747.1; -; Genomic_DNA.
DR   EMBL; CP002987; AFA48975.1; -; Genomic_DNA.
DR   RefSeq; WP_014356575.1; NC_016894.1.
DR   SMR; H6LC27; -.
DR   STRING; 931626.Awo_c22010; -.
DR   TCDB; 3.D.6.1.2; the putative ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family.
DR   EnsemblBacteria; AFA48975; AFA48975; Awo_c22010.
DR   KEGG; awo:Awo_c22010; -.
DR   eggNOG; ENOG4107SVV; Bacteria.
DR   eggNOG; COG2878; LUCA.
DR   KO; K03616; -.
DR   OMA; MLPGANC; -.
DR   OrthoDB; 1619561at2; -.
DR   BioCyc; AWOO931626:G1H37-2276-MONOMER; -.
DR   Proteomes; UP000007177; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.20; -; 1.
DR   HAMAP; MF_00463; RsxB_RnfB; 1.
DR   InterPro; IPR007202; 4Fe-4S_dom.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR011005; Dihydropteroate_synth-like.
DR   InterPro; IPR010207; Elect_transpt_cplx_RnfB/RsxB.
DR   Pfam; PF00037; Fer4; 3.
DR   Pfam; PF13187; Fer4_9; 1.
DR   Pfam; PF04060; FeS; 1.
DR   PROSITE; PS51656; 4FE4S; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 2.
DR   PROSITE; PS51379; 4FE4S_FER_2; 6.
PE   1: Evidence at protein level;
KW   4Fe-4S; Cell membrane; Complete proteome; Electron transport; Iron;
KW   Iron-sulfur; Membrane; Metal-binding; NAD; Reference proteome; Repeat;
KW   Translocase; Transport.
FT   CHAIN         1    333       Na(+)-translocating ferredoxin:NAD(+)
FT                                oxidoreductase complex subunit B.
FT                                /FTId=PRO_0000443517.
FT   DOMAIN       33     92       4Fe-4S. {ECO:0000255|HAMAP-
FT                                Rule:MF_00463}.
FT   DOMAIN      126    162       4Fe-4S ferredoxin-type 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   DOMAIN      163    192       4Fe-4S ferredoxin-type 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   DOMAIN      207    237       4Fe-4S ferredoxin-type 3.
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   DOMAIN      239    266       4Fe-4S ferredoxin-type 4.
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   DOMAIN      270    299       4Fe-4S ferredoxin-type 5.
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   DOMAIN      301    330       4Fe-4S ferredoxin-type 6.
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   REGION        1     27       Hydrophobic. {ECO:0000255|HAMAP-
FT                                Rule:MF_00463}.
FT   METAL        50     50       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL        53     53       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL        58     58       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL        75     75       Iron-sulfur 1 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       138    138       Iron-sulfur 2 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       142    142       Iron-sulfur 2 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       148    148       Iron-sulfur 2 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       152    152       Iron-sulfur 3 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       172    172       Iron-sulfur 3 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       175    175       Iron-sulfur 3 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       178    178       Iron-sulfur 3 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       182    182       Iron-sulfur 2 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       217    217       Iron-sulfur 4 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       220    220       Iron-sulfur 4 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       223    223       Iron-sulfur 4 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       227    227       Iron-sulfur 4 (4Fe-4S).
FT                                {ECO:0000255|HAMAP-Rule:MF_00463}.
FT   METAL       246    246       Iron-sulfur 5 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       249    249       Iron-sulfur 5 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       252    252       Iron-sulfur 5 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       256    256       Iron-sulfur 5 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       279    279       Iron-sulfur 6 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       282    282       Iron-sulfur 6 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       285    285       Iron-sulfur 6 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       289    289       Iron-sulfur 6 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       310    310       Iron-sulfur 7 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       313    313       Iron-sulfur 7 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       316    316       Iron-sulfur 7 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       320    320       Iron-sulfur 7 (4Fe-4S).
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
SQ   SEQUENCE   333 AA;  34727 MW;  138977C70B402B87 CRC64;
     MLNAILVPVG ILGVFGLIFG IGLAIAAKVF EVYEDPRVPL VRAALPGANC GGCGLPGCDA
     LAANIVGGSA AIDACPVGGA SCAAAVAEIM GMEAGSAVKK VATVICQGTC ETAPNRAEYY
     GEMDCREAMI ASGGSKGCRY GCLGYGTCKA VCPFDAIVIG EDGLPKVDPE KCTSCGKCVE
     ACPKSIMTLV PEAQEVIVKC HNFDKGKIAR LSCTTACIAC GACVKACRFD AITVENNCAK
     IDYDKCRQCY ECVDKCPMNC ISGDVEYGKS TAYIIEENCI ACGLCAKNCP VNAITGEIKK
     PPYVIDHDMC IGCGICFDKC RKSAIEMRPN KTK
//
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