ID H8GQ95_METAL Unreviewed; 1009 AA.
AC H8GQ95;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2012, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN ORFNames=Metal_0720 {ECO:0000313|EMBL:EIC28554.1};
OS Methylomicrobium album BG8.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylomicrobium.
OX NCBI_TaxID=686340 {ECO:0000313|EMBL:EIC28554.1, ECO:0000313|Proteomes:UP000005090};
RN [1] {ECO:0000313|EMBL:EIC28554.1, ECO:0000313|Proteomes:UP000005090}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BG8 {ECO:0000313|EMBL:EIC28554.1,
RC ECO:0000313|Proteomes:UP000005090};
RX PubMed=23580712;
RA Kits K.D., Kalyuzhnaya M.G., Klotz M.G., Jetten M.S., Op den Camp H.J.,
RA Vuilleumier S., Bringel F., Dispirito A.A., Murrell J.C., Bruce D.,
RA Cheng J.F., Copeland A., Goodwin L., Hauser L., Lajus A., Land M.L.,
RA Lapidus A., Lucas S., Medigue C., Pitluck S., Woyke T., Zeytun A.,
RA Stein L.Y.;
RT "Genome Sequence of the Obligate Gammaproteobacterial Methanotroph
RT Methylomicrobium album Strain BG8.";
RL Genome Announc. 1:E0017013-E0017013(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
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DR EMBL; CM001475; EIC28554.1; -; Genomic_DNA.
DR RefSeq; WP_005369681.1; NZ_CM001475.1.
DR AlphaFoldDB; H8GQ95; -.
DR STRING; 686340.Metal_0720; -.
DR REBASE; 62990; MalBG8ORF715P.
DR eggNOG; COG0610; Bacteria.
DR HOGENOM; CLU_010804_0_0_6; -.
DR Proteomes; UP000005090; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR040980; SWI2_SNF2.
DR PANTHER; PTHR42927; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42927:SF1; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Helicase {ECO:0000313|EMBL:EIC28554.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000005090};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 306..509
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT REGION 440..459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 888..911
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1009 AA; 114164 MW; D63B6A2E44EC3C12 CRC64;
MVSKTTEKEL EIAIEKQLTG TCLEAQKSVA EVGDLPFSPN HGYQLGLAQD FNARYAIDSK
RFWAFLEHTQ HDGLEKLQKH GGDWRLKVLE RFDKLIKKYG LLHLLKKGLS VDDATLHLMY
PAPLASSSDK VKQNFADNLF SSTRQVRYSL SNTLEEIDLV LFVNGLPFAT LELKNPWSGQ
TARYHGQKQY CEDRDINQPL LQFGRCLVHM AVDTDEIYMT TKLAGQQTFF LPFNQGHNFG
AGNPPNPNGH KSAYLWQAVF SKNSVANIIQ HFVRLDGSSK EPLDKRTLFF PRYHQLDVVR
KLVAHAGQNG VGQTYLIQHS AGSGKSNSIT WAAYQLIEAY PASLDTPGAI ALEKPLFDSV
IVVTDRRLLD KQLRDNIKDF SEVKNIVAPA FKSSELKSAL EQGKKIIITT IQKFPFIIDG
IADLSDKRFA VIIDEAHSSQ SGSAHDNMNR AMGKSEQAEE DAVDVQDKIL QAMQSRKMRG
NASYFAFTAT PKNTTLEKFG VKQADGSFEP FHLYSMKQAI EEGFILDVLA NYTTYKSYYE
IQKSVADNPL FDSAKAQKKL RAFVERHQQT IAVKAEIMLD HFIPQVVNSK KLKGKAKGMV
ITQNIEAAIR YYRAISQLLT DRGNPFKVAI AFSGTKEVDG IEYSEAQINS FAENETRDTF
DKDEYRLLIV ANKYLTGFDQ PKLCAMYVDK KLQGVLAVQA LSRLNRAAPK WGKKTEDLFI
LDFFNSVDDI KTAFDPFYTA TRLSEATDVN VLHELKDALD EVAVYEWFEI EQFVELYFGN
ADAQQLSPII DTAADRFNQE LALDELAKAD FKIKAKQFVK IYGQMAAIMP FEIAAWEKLF
WFLKFLIPKL IVKDPSADRI DELLESVDLS SYGLERIKLN HGIGLDASES ELEPSNSNPR
GTHGGGGERD PLDEIIRSFN ERWFQGWGAT PEAQRIKFIN IADGIKAHPD FQEKYQNNPD
VHTRLLAFEK IFEDVILKNR RNELELYKLL ANDPAFKAAM QQSLRQMVA
//
