ID H8XSS8_FLAIG Unreviewed; 939 AA.
AC H8XSS8;
DT 16-MAY-2012, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2012, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN OrderedLocusNames=KQS_07565 {ECO:0000313|EMBL:CCG53470.1};
OS Flavobacterium indicum (strain DSM 17447 / CIP 109464 / GPTSA100-9).
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1094466 {ECO:0000313|EMBL:CCG53470.1, ECO:0000313|Proteomes:UP000007599};
RN [1] {ECO:0000313|EMBL:CCG53470.1, ECO:0000313|Proteomes:UP000007599}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17447 / CIP 109464 / GPTSA100-9
RC {ECO:0000313|Proteomes:UP000007599};
RX PubMed=22582381; DOI=10.1128/JB.00420-12;
RA Barbier P., Houel A., Loux V., Poulain J., Bernardet J.F., Touchon M.,
RA Duchaud E.;
RT "Complete Genome Sequence of Flavobacterium indicum GPSTA100-9T, Isolated
RT from Warm Spring Water.";
RL J. Bacteriol. 194:3024-3025(2012).
RN [2] {ECO:0000313|Proteomes:UP000007599}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17447 / CIP 109464 / GPTSA100-9
RC {ECO:0000313|Proteomes:UP000007599};
RA Barbier P., Houel A., Loux V., Poulain J., Bernardet J.-F., Touchon M.,
RA Duchaud E.;
RT "Complete genome sequence of Flavobacterium indicum GPTSA100-9T, isolated
RT from warm spring water.";
RL Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; HE774682; CCG53470.1; -; Genomic_DNA.
DR RefSeq; WP_014388595.1; NC_017025.1.
DR AlphaFoldDB; H8XSS8; -.
DR STRING; 1094466.KQS_07565; -.
DR REBASE; 45828; Fin100ORF7535P.
DR KEGG; fin:KQS_07565; -.
DR PATRIC; fig|1094466.5.peg.1488; -.
DR eggNOG; COG4096; Bacteria.
DR HOGENOM; CLU_007363_1_0_10; -.
DR OrthoDB; 9759819at2; -.
DR Proteomes; UP000007599; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd18032; DEXHc_RE_I_III_res; 1.
DR CDD; cd18799; SF2_C_EcoAI-like; 1.
DR Gene3D; 3.90.1570.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013670; EcoEI_R_C_dom.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR PANTHER; PTHR47396:SF1; ATP-DEPENDENT HELICASE IRC3-RELATED; 1.
DR PANTHER; PTHR47396; TYPE I RESTRICTION ENZYME ECOKI R PROTEIN; 1.
DR Pfam; PF08463; EcoEI_R_C; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CCG53470.1};
KW Methyltransferase {ECO:0000313|EMBL:CCG53470.1};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000007599};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW Transferase {ECO:0000313|EMBL:CCG53470.1}.
FT DOMAIN 172..361
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 939 AA; 108535 MW; 7C9204ED8A523EFE CRC64;
MTNQNPEQIA RDEIDKQLLA CGWVIQNKSQ INLAANIGVA VREYQTNVGP ADYVLFVNQT
PVGVIEAKRS ELGQSLSMVE EQGEDYAKAK LKYLNNDPLP FVYISTGDIT KFTNYKDPKP
RAREVFTFHR PETLASWIKN EKTLRAGLLD IPQLPIDGLR DCQIDTITEL EKSFKDFRPK
SLVQMATGSG KTFTAITFIY RLLKFAKAKR ILFLVDTKNL GEQAEQEFMA YLPNDSNSKF
TELHPVTRLK SSFIPQDNQV YISTIQRMYS ILKGNEMDES AEELNPYEYV QTKEPLPVVY
NEKIPIEFFD FIVIDECHRS IYNLWRQVLD YFDAFQVGLT ATPDNRTFMY FNQNVVSDYG
YEKAVVDGVL VPYNVFTIET KISKEGAAIG VGEQVDKRER LTRKKFWETV DEDIAYSGRQ
LDKDIVNPST IRTIVKAVRD NLPNMFPDRF DEHGNFEVPK MLIFAKSDSH AEDIIDIVRE
EFGEENKFCK KITYQSTEDP KSILSQFRNE YYPRVAVTVD MIATGTDIRP LEVLLFMRDV
KSKSYYEQMK GRGTRTCTLE ELRKTGTPTA KFNKDHFVII DAIGVEKSQK TDSRPLEKAP
GVALKDVLGN IAMGNRSEEM LTTLANRLIR LDRQINPKEK EKFAELANGL EIKQVVQKLL
QAYDPDVREQ LAISCQQKML GQAPAVIEQA IETEHRAIIE QAVAVLHNPD LRNYIIDVRK
KYDQIIDTLN IDEVTNMGWV KDVTEQAQSI VTAFETWVAN NQDEALALQI FYDEPYRRRE
LTYKMIRDLY EKLVLEQPTL NAPKLWDAYA ELEDNQDKDK FVKRMPRTEK PKQDLVLLVS
VIRKALKIDE YLTAYDKTVD RNFQEWIFKK NAGQHNTYTE EQMQWLRMIK DYIANSFHLD
TEDFELNPFN AYGGLGKFYQ LFGNQYIEII EELNEVLAA
//
