ID HBD_SYNWW Reviewed; 279 AA.
AC Q0AVM2;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 101.
DE RecName: Full=3-hydroxybutyryl-CoA dehydrogenase {ECO:0000303|PubMed:23468890};
DE EC=1.1.1.35 {ECO:0000305|PubMed:23468890};
GN OrderedLocusNames=Swol_1935 {ECO:0000312|EMBL:ABI69232.1};
OS Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Syntrophomonadaceae;
OC Syntrophomonas.
OX NCBI_TaxID=335541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2245B / Goettingen;
RX PubMed=21966920; DOI=10.1111/j.1462-2920.2010.02237.x;
RA Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L.,
RA McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.;
RT "The genome of Syntrophomonas wolfei: new insights into syntrophic
RT metabolism and biohydrogen production.";
RL Environ. Microbiol. 12:2289-2301(2010).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, SUBCELLULAR LOCATION,
RP FUNCTION, AND PATHWAY.
RX PubMed=23468890; DOI=10.1371/journal.pone.0056905;
RA Schmidt A., Mueller N., Schink B., Schleheck D.;
RT "A proteomic view at the biochemistry of syntrophic butyrate oxidation in
RT Syntrophomonas wolfei.";
RL PLoS ONE 8:E56905-E56905(2013).
CC -!- FUNCTION: Involved in syntrophic growth of S.wolfei with butyrate, as
CC part of the butyrate oxidation pathway. Probably catalyzes the
CC oxidation of 3-hydroxybutyryl-CoA to acetoacetyl-CoA.
CC {ECO:0000305|PubMed:23468890}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:42048, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:78611;
CC Evidence={ECO:0000305|PubMed:23468890};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000305|PubMed:23468890};
CC -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC {ECO:0000305|PubMed:23468890}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23468890}.
CC -!- INDUCTION: Highly expressed during syntrophic growth with butyrate (at
CC protein level). Seems to be constitutively expressed.
CC {ECO:0000269|PubMed:23468890}.
CC -!- SIMILARITY: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; CP000448; ABI69232.1; -; Genomic_DNA.
DR RefSeq; WP_011641325.1; NC_008346.1.
DR AlphaFoldDB; Q0AVM2; -.
DR SMR; Q0AVM2; -.
DR STRING; 335541.Swol_1935; -.
DR KEGG; swo:Swol_1935; -.
DR eggNOG; COG1250; Bacteria.
DR HOGENOM; CLU_009834_2_0_9; -.
DR OrthoDB; 9771883at2; -.
DR UniPathway; UPA00863; -.
DR Proteomes; UP000001968; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0044248; P:cellular catabolic process; IEA:UniProt.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR022694; 3-OHacyl-CoA_DH.
DR InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR48075; 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN; 1.
DR PANTHER; PTHR48075:SF5; 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR PIRSF; PIRSF000105; HCDH; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00067; 3HCDH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Fatty acid metabolism; Lipid metabolism; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..279
FT /note="3-hydroxybutyryl-CoA dehydrogenase"
FT /id="PRO_0000442214"
SQ SEQUENCE 279 AA; 29762 MW; 78205CA5EA5B92B0 CRC64;
MKIMVLGAGT MGAGIVQTAA QAGFEVVVRD IKQEFVDRGI AGIDKLLSKN VDKGRMTAED
KAAVMGRISG TVDMGAAADC DLVIEAALEV MDIKKAIFKE LDSICKPECI LASNTSALSV
TEIAAATGRA DKVIGMHFFN PVPAMKLVEV IRGASTSQAT YDAIKDLSVK MGKSPVEINE
APGFVVNRLL IPMLNEGMYC LMEGVANAAD IDTSMKFGAG HPMGPLALAD MIGLDICLKI
METLYKEFGD PKYRPCPLLA KMVRANKLGR KTGEGFFAY
//
