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Database: UniProt
Entry: HEI10_ORYSJ
LinkDB: HEI10_ORYSJ
Original site: HEI10_ORYSJ 
ID   HEI10_ORYSJ             Reviewed;         303 AA.
AC   I6PL68; A0A0P0VGR2; B9F4F5; I6PII6; Q6EUG3;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2019, sequence version 2.
DT   13-NOV-2019, entry version 44.
DE   RecName: Full=E3 ubiquitin-protein ligase CCNB1IP1 homolog {ECO:0000305};
DE            EC=2.3.2.27 {ECO:0000305};
DE   AltName: Full=RING finger-containing protein HEI10 {ECO:0000303|PubMed:22792078};
DE            Short=OsHEI10 {ECO:0000303|PubMed:31266799};
DE   AltName: Full=RING-type E3 ubiquitin transferase HEI10 {ECO:0000305};
GN   Name=HEI10 {ECO:0000303|PubMed:22792078};
GN   OrderedLocusNames=Os02g0232100 {ECO:0000312|EMBL:BAS77787.1},
GN   LOC_Os02g13810 {ECO:0000305};
GN   ORFNames=OJ1705_E12.17 {ECO:0000312|EMBL:BAD27706.1},
GN   OsJ_05990 {ECO:0000312|EMBL:EEE56612.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, DISRUPTION
RP   PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nipponbare, and cv. Wuxiangjing 9;
RX   PubMed=22792078; DOI=10.1371/journal.pgen.1002809;
RA   Wang K., Wang M., Tang D., Shen Y., Miao C., Hu Q., Lu T., Cheng Z.;
RT   "The role of rice HEI10 in the formation of meiotic crossovers.";
RL   PLoS Genet. 8:E1002809-E1002809(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H.,
RA   Cong L., Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J.,
RA   Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X.,
RA   Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y.,
RA   Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J.,
RA   Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y.,
RA   Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y.,
RA   Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z.,
RA   Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T.,
RA   Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H.,
RA   Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W.,
RA   Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L.,
RA   Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J.,
RA   Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   INTERACTION WITH ZIP4 AND PTD.
RX   PubMed=31266799; DOI=10.1104/pp.19.00082;
RA   Zhang J., Wang C., Higgins J.D., Kim Y.J., Moon S., Jung K.H., Qu S.,
RA   Liang W.;
RT   "A multiprotein complex regulates interference-sensitive crossover
RT   formation in rice.";
RL   Plant Physiol. 181:221-235(2019).
CC   -!- FUNCTION: Ubiquitin E3 ligase required for class I crossover (CO)
CC       formation during meiosis. {ECO:0000269|PubMed:22792078}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor
CC         protein]-L-lysine.; EC=2.3.2.27;
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000305}.
CC   -!- SUBUNIT: Interacts with ZIP4 and PTD.
CC       {ECO:0000269|PubMed:31266799}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22792078}.
CC       Chromosome {ECO:0000269|PubMed:22792078}. Note=Dynamic
CC       localization on the meiotic chromosomes. Initially appears as
CC       distinct foci. Later observed along the chromosomes and finally
CC       restrict to prominent foci that specially localize to chiasma
CC       sites. Extends along the chromosome in the wake of synapsis.
CC       {ECO:0000269|PubMed:22792078}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=HEI10A;
CC         IsoId=I6PL68-1; Sequence=Displayed;
CC       Name=2; Synonyms=HEI10B;
CC         IsoId=I6PL68-2; Sequence=VSP_053850;
CC         Note=No experimental confirmation available.;
CC   -!- TISSUE SPECIFICITY: Expressed in young panicles.
CC       {ECO:0000269|PubMed:22792078}.
CC   -!- DISRUPTION PHENOTYPE: The mutants hei10-1 and hei10-2 have normal
CC       vegetative growth but exhibited complete sterility, due to
CC       shrunken and inviable pollen as well as sterile female gametes.
CC       Reduced chiasma frequency, with a random distribution among cells
CC       of remaining chiasmata, but normal early recombination events and
CC       synaptonemal complex (SC) formation.
CC       {ECO:0000269|PubMed:22792078}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EEE56612.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; JQ624877; AFJ04809.1; -; mRNA.
DR   EMBL; JQ624878; AFJ04810.1; -; mRNA.
DR   EMBL; AP004070; BAD27706.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF08285.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS77787.1; -; Genomic_DNA.
DR   EMBL; CM000139; EEE56612.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_015623603.1; XM_015768117.1. [I6PL68-1]
DR   RefSeq; XP_015623604.1; XM_015768118.1.
DR   RefSeq; XP_015623605.1; XM_015768119.1. [I6PL68-2]
DR   SMR; I6PL68; -.
DR   STRING; 4530.OS02T0232100-01; -.
DR   PaxDb; I6PL68; -.
DR   EnsemblPlants; Os02t0232100-01; Os02t0232100-01; Os02g0232100.
DR   GeneID; 4328810; -.
DR   Gramene; Os02t0232100-01; Os02t0232100-01; Os02g0232100.
DR   KEGG; osa:4328810; -.
DR   eggNOG; ENOG410IE9K; Eukaryota.
DR   eggNOG; ENOG410XTEP; LUCA.
DR   KO; K10639; -.
DR   OrthoDB; 1383870at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000059680; Chromosome 2.
DR   Genevisible; I6PL68; OS.
DR   GO; GO:0005712; C:chiasma; IDA:UniProtKB.
DR   GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR   GO; GO:0007129; P:synapsis; IDA:UniProtKB.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF14634; zf-RING_5; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chromosome; Coiled coil; Complete proteome;
KW   DNA recombination; Meiosis; Metal-binding; Nucleus;
KW   Reference proteome; Transferase; Ubl conjugation pathway; Zinc;
KW   Zinc-finger.
FT   CHAIN         1    303       E3 ubiquitin-protein ligase CCNB1IP1
FT                                homolog.
FT                                /FTId=PRO_0000425731.
FT   ZN_FING       3     42       RING-type; degenerate.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00175}.
FT   COILED      119    184       {ECO:0000255}.
FT   VAR_SEQ     214    222       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:22792078}.
FT                                /FTId=VSP_053850.
FT   CONFLICT    303    303       T -> TL (in Ref. 1; AFJ04809/AFJ04810).
FT                                {ECO:0000305}.
SQ   SEQUENCE   303 AA;  34409 MW;  71B3F78707E74C58 CRC64;
     MKCNACWREL EGQAVSTTCG HLLCTEDAKK ILSNDAACPI CDQVLSKSHM RPVDTNPNDD
     WTNMSMAGVS PQILMKSAYR SVMFYIGQKE LEMQYKMNRI VGQCRQKCEL MQAKFTEKLE
     EVHTAYQKMA KKCQLMEQEV ENLSRDKQEL QEKFAEKSRQ KRKLDEMYDQ LRSEYESAKR
     SAIQPANNYF PRAQPDLFSG VPNIMDSSDP LRQGLAGLPE TPGRRDEGWA PPPRQRRSTS
     GPFELSAGSP AHNAAPPVDI RPRQPARPVF GTAMNNTSAA LRNMIISPVK RPQLSRNRPH
     MFT
//
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