ID HFA2E_ORYSJ Reviewed; 357 AA.
AC Q6F388; A0A0P0W424;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 27-MAR-2024, entry version 138.
DE RecName: Full=Heat stress transcription factor A-2e;
DE AltName: Full=Heat stress transcription factor 12;
DE Short=OsHsf-12;
GN Name=HSFA2E; Synonyms=HSF12;
GN OrderedLocusNames=Os03g0795900, LOC_Os03g58160;
GN ORFNames=OsJ_012388, OSJNBb0021G19.9;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15625403; DOI=10.1007/bf02712120;
RA Baniwal S.K., Bharti K., Chan K.Y., Fauth M., Ganguli A., Kotak S.,
RA Mishra S.K., Nover L., Port M., Scharf K.-D., Tripp J., Weber C.,
RA Zielinski D., von Koskull-Doering P.;
RT "Heat stress response in plants: a complex game with chaperones and more
RT than twenty heat stress transcription factors.";
RL J. Biosci. 29:471-487(2004).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=18064488; DOI=10.1007/s00425-007-0670-4;
RA Yokotani N., Ichikawa T., Kondou Y., Matsui M., Hirochika H., Iwabuchi M.,
RA Oda K.;
RT "Expression of rice heat stress transcription factor OsHsfA2e enhances
RT tolerance to environmental stresses in transgenic Arabidopsis.";
RL Planta 227:957-967(2008).
CC -!- FUNCTION: Transcriptional activator expressed upon environmental stress
CC that specifically binds DNA of heat shock promoter elements (HSE).
CC Involved in heat stress response. {ECO:0000269|PubMed:18064488}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:18064488}. Nucleus
CC {ECO:0000269|PubMed:18064488}.
CC -!- INDUCTION: By heat stress. {ECO:0000269|PubMed:18064488}.
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain. AHA motifs are transcriptional activator
CC elements. {ECO:0000269|PubMed:18407058}.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC {ECO:0000305}.
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DR EMBL; AC092076; AAT76423.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99338.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13466.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS86837.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ28905.1; -; Genomic_DNA.
DR EMBL; AK068660; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015630486.1; XM_015775000.1.
DR AlphaFoldDB; Q6F388; -.
DR SMR; Q6F388; -.
DR STRING; 39947.Q6F388; -.
DR PaxDb; 39947-Q6F388; -.
DR EnsemblPlants; Os03t0795900-01; Os03t0795900-01; Os03g0795900.
DR GeneID; 4334418; -.
DR Gramene; Os03t0795900-01; Os03t0795900-01; Os03g0795900.
DR KEGG; osa:4334418; -.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_030308_1_0_1; -.
DR InParanoid; Q6F388; -.
DR OMA; MADMPQP; -.
DR OrthoDB; 1117127at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q6F388; OS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; HEAT SHOCK TRANSCRIPTION FACTOR; 1.
DR PANTHER; PTHR10015:SF450; HEAT STRESS TRANSCRIPTION FACTOR A-2E; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Activator; Coiled coil; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..357
FT /note="Heat stress transcription factor A-2e"
FT /id="PRO_0000350825"
FT REGION 158..208
FT /note="Hydrophobic repeat HR-A/B"
FT REGION 231..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 150..202
FT /evidence="ECO:0000255"
FT MOTIF 223..238
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 314..323
FT /note="AHA"
FT MOTIF 341..348
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 275..306
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..334
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 343..357
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 124
FT /note="S -> G (in Ref. 6; AK068660)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 357 AA; 40259 MW; C815B71EDD576AF8 CRC64;
MNYRVVNPVK VESGPSTGVA NGQPPRPMDG LADGGPPPFL TKTYDMVDDP TTDAVVSWSA
TNNSFVVWDP HLFGNVLLPR YFKHNNFSSF VRQLNTYGFR KVDPDKWEFA NEGFLRGQKH
LLKSIKRRKP PNSSPSQQSL GSFLEVGHFG YEGEIDQLKR DKHLLMAEVV KLRQEQQNTK
SDLQAMEQKL QGTEQKQQHM MAFLSRVMHN PEFIRQLFSQ SEMRKELEEF VSKKRRRRID
QGPELDSMGT GSSPEQVSQV MFEPHDPVDS LFNGVPSDLE SSSVEANGGK AQQDVASSSS
EHGKIKPSNG ELNEDFWEDL LHEGGLDEDT RNPAIDDMNL LSQKMGYLNS SSTKSPQ
//
