ID HMDH1_ORYSJ Reviewed; 532 AA.
AC Q0DY59; P48019;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 24-JAN-2024, entry version 105.
DE RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 1;
DE Short=HMG-CoA reductase 1;
DE EC=1.1.1.34;
GN Name=HMG1; OrderedLocusNames=Os02g0713900, LOC_Os02g48330;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor
CC of all isoprenoid compounds present in plants.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-
CC methylglutaryl-CoA + 2 H(+) + 2 NADPH; Xref=Rhea:RHEA:15989,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:36464, ChEBI:CHEBI:43074,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.34;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10003};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the HMG-CoA reductase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF09829.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP008208; BAF09829.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014958; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015623837.1; XM_015768351.1.
DR AlphaFoldDB; Q0DY59; -.
DR SMR; Q0DY59; -.
DR STRING; 39947.Q0DY59; -.
DR GlyCosmos; Q0DY59; 2 sites, No reported glycans.
DR PaxDb; 39947-Q0DY59; -.
DR GeneID; 9272427; -.
DR KEGG; osa:9272427; -.
DR eggNOG; KOG2480; Eukaryota.
DR InParanoid; Q0DY59; -.
DR OrthoDB; 816560at2759; -.
DR PlantReactome; R-OSA-1119615; Mevalonate pathway.
DR UniPathway; UPA00058; UER00103.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0005778; C:peroxisomal membrane; IBA:GO_Central.
DR GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IBA:GO_Central.
DR GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR GO; GO:0008299; P:isoprenoid biosynthetic process; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR CDD; cd00643; HMG-CoA_reductase_classI; 1.
DR Gene3D; 1.10.3270.10; HMGR, N-terminal domain; 1.
DR Gene3D; 3.30.70.420; Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain; 1.
DR InterPro; IPR002202; HMG_CoA_Rdtase.
DR InterPro; IPR023074; HMG_CoA_Rdtase_cat_sf.
DR InterPro; IPR023076; HMG_CoA_Rdtase_CS.
DR InterPro; IPR004554; HMG_CoA_Rdtase_eu_arc.
DR InterPro; IPR023282; HMG_CoA_Rdtase_N.
DR InterPro; IPR009023; HMG_CoA_Rdtase_NAD(P)-bd_sf.
DR InterPro; IPR009029; HMG_CoA_Rdtase_sub-bd_dom_sf.
DR NCBIfam; TIGR00533; HMG_CoA_R_NADP; 1.
DR PANTHER; PTHR10572; 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE; 1.
DR PANTHER; PTHR10572:SF14; 3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE 1; 1.
DR Pfam; PF00368; HMG-CoA_red; 1.
DR PRINTS; PR00071; HMGCOARDTASE.
DR SUPFAM; SSF55035; NAD-binding domain of HMG-CoA reductase; 1.
DR SUPFAM; SSF56542; Substrate-binding domain of HMG-CoA reductase; 1.
DR PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; NADP;
KW Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..532
FT /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase 1"
FT /id="PRO_0000114446"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 77..117
FT /note="Linker"
FT /evidence="ECO:0000250"
FT REGION 78..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..532
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT ACT_SITE 211
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 343
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 419
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 517
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10003"
FT CARBOHYD 275
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 521
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 532 AA; 55615 MW; D70406C15BA50127 CRC64;
MDVRRGGGGG RIVGAARRAL TWGALPLPMR ITNGLAMVSL VLSSCDLLRL CSDRERPLGG
REFATVVYLV SLFAHPDAPA TTTGDDDDGQ GGSRRARPAA AEPAPMHGHG GGMMEADDEE
IVAAVASGAL PSHRLESRLG DCRRAARLRR EALRRVTGRG VEGLPFDGMD YQAILGQCCE
MPVGYVQLPV GVAGPLLLDG REYHVPMATT EGCLVASVNR GCRAISASGG AFSVLLRDAM
SRAPAVKLPS AMRAAELKAF AEAPANFELL AAVFNRSSRF GRLQDIRCAL AGRNLYMRFS
CITGDAMGMN MVSKGVENVL GYLQNVFPDM DVISVSGNYC SDKKPTAVNW IEGRGKSVVC
EAIIKGDVVQ KVLKTTVEKL VELNIIKNLA GSAVAGALGG FNAHASNIVT ALFIATGQDP
AQNVESSQCI TMLEEVNDGD DLHISVTMPS IEVGTIGGGT CLASQAACLN LLGVKGSNHG
SPGANAKRLA TIVAGSVLAG ELSLLAALAS GHLVKSHMMY NRSSKDVAKA AS
//
