ID HPF_MYCTU Reviewed; 219 AA.
AC O05886; F2GKB7; I6X6S6; L0TER5;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 4.
DT 08-NOV-2023, entry version 135.
DE RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
GN Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839}; OrderedLocusNames=Rv3241c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP PROTEIN SEQUENCE OF 4-16, AND SEQUENCE REVISION TO N-TERMINUS.
RC STRAIN=H37Rv;
RX PubMed=34915127; DOI=10.1016/j.ygeno.2021.12.001;
RA Shi J., Meng S., Wan L., Zhang Z., Jiang S., Zhu H., Dai E., Chang L.,
RA Gao H., Wan K., Zhang L., Zhao X., Liu H., Lyu Z., Zhang Y., Xu P.;
RT "Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct
RT annotated encoding genes.";
RL Genomics 114:292-304(2022).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011445;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC ribosomes in stationary phase; 100S ribosomes are translationally
CC inactive and sometimes present during exponential growth.
CC {ECO:0000255|HAMAP-Rule:MF_00839}.
CC -!- SUBUNIT: Interacts with 100S ribosomes. {ECO:0000255|HAMAP-
CC Rule:MF_00839}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCP46060.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
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DR EMBL; AL123456; CCP46060.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_217758.3; NC_000962.3.
DR AlphaFoldDB; O05886; -.
DR SMR; O05886; -.
DR STRING; 83332.Rv3241c; -.
DR PaxDb; 83332-Rv3241c; -.
DR DNASU; 888849; -.
DR GeneID; 888849; -.
DR KEGG; mtu:Rv3241c; -.
DR PATRIC; fig|83332.12.peg.3624; -.
DR TubercuList; Rv3241c; -.
DR eggNOG; COG1544; Bacteria.
DR InParanoid; O05886; -.
DR OrthoDB; 9794975at2; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR GO; GO:0045900; P:negative regulation of translational elongation; IBA:GO_Central.
DR GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR CDD; cd00552; RaiA; 1.
DR Gene3D; 3.30.160.100; Ribosome hibernation promotion factor-like; 1.
DR Gene3D; 3.30.505.50; Sigma 54 modulation/S30EA ribosomal protein, C-terminal domain; 1.
DR HAMAP; MF_00839; HPF; 1.
DR InterPro; IPR034694; HPF_long/plastid.
DR InterPro; IPR036567; RHF-like.
DR InterPro; IPR003489; RHF/RaiA.
DR InterPro; IPR032528; Ribosom_S30AE_C.
DR InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR NCBIfam; TIGR00741; yfiA; 1.
DR PANTHER; PTHR33231; 30S RIBOSOMAL PROTEIN; 1.
DR PANTHER; PTHR33231:SF1; 30S RIBOSOMAL PROTEIN; 1.
DR Pfam; PF16321; Ribosom_S30AE_C; 1.
DR Pfam; PF02482; Ribosomal_S30AE; 1.
DR SUPFAM; SSF69754; Ribosome binding protein Y (YfiA homologue); 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Reference proteome;
KW Translation regulation.
FT CHAIN 1..219
FT /note="Ribosome hibernation promotion factor"
FT /id="PRO_0000436446"
SQ SEQUENCE 219 AA; 25093 MW; CC6E9B0D0B2A0854 CRC64;
MSRLAVDSGQ VLAEPKSNAE IVFKGRNVEI PDHFRIYVSQ KLARLERFDR TIYLFDVELD
HERNRRQRKS CQRVEITARG RGPVVRGEAC ADSFYAALES AVVKLESRLR RGKDRRKVHY
GDKTPVSLAE ATAVVPAPEN GFNTRPAEAH DHDGAVVERE PGRIVRTKEH PAKPMSVDDA
LYQMELVGHD FFLFYDKDTE RPSVVYRRHA YDYGLIRLA
//
