ID HSP74_YEAST Reviewed; 642 AA.
AC P22202; D3DM10;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 27-MAR-2024, entry version 209.
DE RecName: Full=Heat shock protein SSA4;
GN Name=SSA4; OrderedLocusNames=YER103W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DS10;
RX PubMed=2121731; DOI=10.1016/s0021-9258(17)30603-8;
RA Boorstein W.R., Craig E.A.;
RT "Structure and regulation of the SSA4 HSP70 gene of Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 265:18912-18921(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-552, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: HSP SSA4 expression is restricted to conditions of stress.
CC -!- MISCELLANEOUS: Present with 17900 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; J05637; AAA63574.1; -; Genomic_DNA.
DR EMBL; U18839; AAB64658.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07764.1; -; Genomic_DNA.
DR PIR; B36590; B36590.
DR RefSeq; NP_011029.3; NM_001178994.3.
DR PDB; 3UPV; X-ray; 1.60 A; B=636-642.
DR PDBsum; 3UPV; -.
DR AlphaFoldDB; P22202; -.
DR SMR; P22202; -.
DR BioGRID; 36849; 138.
DR DIP; DIP-3916N; -.
DR IntAct; P22202; 35.
DR MINT; P22202; -.
DR STRING; 4932.YER103W; -.
DR iPTMnet; P22202; -.
DR MaxQB; P22202; -.
DR PaxDb; 4932-YER103W; -.
DR PeptideAtlas; P22202; -.
DR EnsemblFungi; YER103W_mRNA; YER103W; YER103W.
DR GeneID; 856840; -.
DR KEGG; sce:YER103W; -.
DR AGR; SGD:S000000905; -.
DR SGD; S000000905; SSA4.
DR VEuPathDB; FungiDB:YER103W; -.
DR eggNOG; KOG0101; Eukaryota.
DR GeneTree; ENSGT00940000176322; -.
DR HOGENOM; CLU_005965_3_2_1; -.
DR InParanoid; P22202; -.
DR OMA; PCQSVNP; -.
DR OrthoDB; 143at2759; -.
DR BioCyc; YEAST:G3O-30268-MONOMER; -.
DR Reactome; R-SCE-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-SCE-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-SCE-3371568; Attenuation phase.
DR BioGRID-ORCS; 856840; 6 hits in 10 CRISPR screens.
DR PRO; PR:P22202; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P22202; Protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0031072; F:heat shock protein binding; IBA:GO_Central.
DR GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; IGI:SGD.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0006457; P:protein folding; IGI:SGD.
DR GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IMP:SGD.
DR CDD; cd10233; HSPA1-2_6-8-like_NBD; 1.
DR Gene3D; 1.20.1270.10; -; 1.
DR Gene3D; 3.30.30.30; -; 1.
DR Gene3D; 3.30.420.40; -; 2.
DR IDEAL; IID50203; -.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029048; HSP70_C_sf.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; HEAT SHOCK PROTEIN 70KDA; 1.
DR PANTHER; PTHR19375:SF385; HEAT SHOCK PROTEIN SSA3-RELATED; 1.
DR Pfam; PF00012; HSP70; 1.
DR PRINTS; PR00301; HEATSHOCK70.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR SUPFAM; SSF100934; Heat shock protein 70kD (HSP70), C-terminal subdomain; 1.
DR SUPFAM; SSF100920; Heat shock protein 70kD (HSP70), peptide-binding domain; 1.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Stress response.
FT CHAIN 1..642
FT /note="Heat shock protein SSA4"
FT /id="PRO_0000078388"
FT REGION 608..642
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 552
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT HELIX 637..639
FT /evidence="ECO:0007829|PDB:3UPV"
SQ SEQUENCE 642 AA; 69651 MW; 7A976357AC35C352 CRC64;
MSKAVGIDLG TTYSCVAHFA NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAMN
PHNTVFDAKR LIGRKFDDPE VTNDAKHYPF KVIDKGGKPV VQVEYKGETK TFTPEEISSM
ILTKMKETAE NFLGTEVKDA VVTVPAYFND SQRQATKDAG TIAGLNVLRI INEPTAAAIA
YGLDKKSQKE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDSRLVNFLA
EEFKRKNKKD LTTNQRSLRR LRTAAERAKR TLSSSAQTSI EIDSLFEGID FYTSITRARF
EELCADLFRS TLEPVEKVLA DSKLDKSQID EIVLVGGSTR IPKVQKLVSD FFNGKEPNRS
INPDEAVAYG AAVQAAILTG DQSSTTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT
KKSEVFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIEVTFDIDA
NGILNVSAVE KGTGKSNKIT ITNDKGRLSK EDIDKMVAEA EKFKAEDEQE AQRVQAKNQL
ESYAFTLKNS VSENNFKEKV GEEDARKLEA AAQDAINWLD ASQAASTEEY KERQKELEGV
ANPIMSKFYG AAGGAPGAGP VPGAGAGPTG APDNGPTVEE VD
//
