ID HYPA2_BRADU Reviewed; 113 AA.
AC Q45256;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 116.
DE RecName: Full=Hydrogenase maturation factor HypA 2 {ECO:0000255|HAMAP-Rule:MF_00213};
GN Name=hypA2 {ECO:0000255|HAMAP-Rule:MF_00213}; Synonyms=hypA;
GN OrderedLocusNames=bll6932;
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=224911;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=8305450; DOI=10.1016/0005-2728(94)90163-5;
RA Fu C., Maier R.J.;
RT "Nucleotide sequences of two hydrogenase-related genes (hypA and hypB) from
RT Bradyrhizobium japonicum, one of which (hypB) encodes an extremely
RT histidine-rich region and guanine nucleotide-binding domains.";
RL Biochim. Biophys. Acta 1184:135-138(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
CC -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC for nickel insertion into the metal center of the hydrogenase.
CC {ECO:0000255|HAMAP-Rule:MF_00213}.
CC -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC Rule:MF_00213, ECO:0000305}.
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DR EMBL; L24513; AAA17762.1; -; Unassigned_DNA.
DR EMBL; BA000040; BAC52197.1; -; Genomic_DNA.
DR RefSeq; NP_773572.1; NC_004463.1.
DR RefSeq; WP_011089670.1; NZ_CP011360.1.
DR AlphaFoldDB; Q45256; -.
DR SMR; Q45256; -.
DR STRING; 224911.AAV28_32250; -.
DR EnsemblBacteria; BAC52197; BAC52197; BAC52197.
DR GeneID; 64026688; -.
DR KEGG; bja:bll6932; -.
DR PATRIC; fig|224911.44.peg.6966; -.
DR eggNOG; COG0375; Bacteria.
DR HOGENOM; CLU_126929_0_0_5; -.
DR InParanoid; Q45256; -.
DR OrthoDB; 288014at2; -.
DR PhylomeDB; Q45256; -.
DR Proteomes; UP000002526; Chromosome.
DR GO; GO:0016151; F:nickel cation binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR GO; GO:0051604; P:protein maturation; IBA:GO_Central.
DR GO; GO:0036211; P:protein modification process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.2320.80; -; 1.
DR HAMAP; MF_00213; HypA_HybF; 1.
DR InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR InterPro; IPR000688; HypA/HybF.
DR NCBIfam; TIGR00100; hypA; 1.
DR PANTHER; PTHR34535; HYDROGENASE MATURATION FACTOR HYPA; 1.
DR PANTHER; PTHR34535:SF3; HYDROGENASE MATURATION FACTOR HYPA; 1.
DR Pfam; PF01155; HypA; 1.
DR PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR PROSITE; PS01249; HYPA; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nickel; Reference proteome; Zinc.
FT CHAIN 1..113
FT /note="Hydrogenase maturation factor HypA 2"
FT /id="PRO_0000129035"
FT BINDING 2
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 73
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 76
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 92
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ SEQUENCE 113 AA; 12331 MW; 5B2CFA98F1142601 CRC64;
MHEMALCEGI IGIVEEEARK RAFAKVNVVC LEIGALSHVA PEALQFCFEA VAARTIAQGA
KLEIVETPGT AWCMACSKSV EIKQRYEPCP SCGGYQLQVT GGEEMRVREL EVD
//
