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Database: UniProt
Entry: I0GAG2_9BRAD
LinkDB: I0GAG2_9BRAD
Original site: I0GAG2_9BRAD 
ID   I0GAG2_9BRAD            Unreviewed;       568 AA.
AC   I0GAG2;
DT   13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT   13-JUN-2012, sequence version 1.
DT   08-MAY-2019, entry version 58.
DE   RecName: Full=Urease subunit alpha {ECO:0000256|HAMAP-Rule:MF_01953};
DE            EC=3.5.1.5 {ECO:0000256|HAMAP-Rule:MF_01953};
DE   AltName: Full=Urea amidohydrolase subunit alpha {ECO:0000256|HAMAP-Rule:MF_01953};
GN   Name=ureC {ECO:0000256|HAMAP-Rule:MF_01953};
GN   ORFNames=S23_45550 {ECO:0000313|EMBL:BAL77749.1};
OS   Bradyrhizobium sp. S23321.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=335659 {ECO:0000313|EMBL:BAL77749.1, ECO:0000313|Proteomes:UP000007886};
RN   [1] {ECO:0000313|EMBL:BAL77749.1, ECO:0000313|Proteomes:UP000007886}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S23321 {ECO:0000313|EMBL:BAL77749.1,
RC   ECO:0000313|Proteomes:UP000007886};
RX   PubMed=22452844; DOI=10.1264/jsme2.ME11321;
RA   Okubo T., Tsukui T., Maita H., Okamoto S., Oshima K., Fujisawa T.,
RA   Saito A., Futamata H., Hattori R., Shimomura Y., Haruta S.,
RA   Morimoto S., Wang Y., Sakai Y., Hattori M., Aizawa S.,
RA   Nagashima K.V.P., Masuda S., Hattori T., Yamashita A., Bao Z.,
RA   Hayatsu M., Kajiya-Kanegae H., Yoshinaga I., Sakamoto K., Toyota K.,
RA   Nakao M., Kohara M., Anda M., Niwa R., Jung-Hwan P.,
RA   Sameshima-Saito R., Tokuda S., Yamamoto S., Yamamoto S., Yokoyama T.,
RA   Akutsu T., Nakamura Y., Nakahira-Yanaka Y., Takada Hoshino Y.,
RA   Hirakawa H., Mitsui H., Terasawa K., Itakura M., Sato S.,
RA   Ikeda-Ohtsubo W., Sakakura N., Kaminuma E., Minamisawa K.;
RT   "Complete genome sequence of Bradyrhizobium sp. S23321: insights into
RT   symbiosis evolution in soil oligotrophs.";
RL   Microbes Environ. 27:306-315(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|HAMAP-Rule:MF_01953,
CC         ECO:0000256|SAAS:SAAS01119912};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01953,
CC         ECO:0000256|PIRSR:PIRSR611612-51};
CC       Note=Binds 2 nickel ions per subunit. {ECO:0000256|HAMAP-
CC       Rule:MF_01953, ECO:0000256|PIRSR:PIRSR611612-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1. {ECO:0000256|HAMAP-
CC       Rule:MF_01953, ECO:0000256|SAAS:SAAS00317636}.
CC   -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC
CC       (alpha) subunits. Three heterotrimers associate to form the active
CC       enzyme. {ECO:0000256|HAMAP-Rule:MF_01953}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01953,
CC       ECO:0000256|PROSITE-ProRule:PRU00700,
CC       ECO:0000256|SAAS:SAAS00548017}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|HAMAP-Rule:MF_01953,
CC       ECO:0000256|PIRSR:PIRSR611612-50}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases
CC       superfamily. Urease alpha subunit family. {ECO:0000256|HAMAP-
CC       Rule:MF_01953, ECO:0000256|RuleBase:RU004158,
CC       ECO:0000256|SAAS:SAAS00849550}.
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DR   EMBL; AP012279; BAL77749.1; -; Genomic_DNA.
DR   RefSeq; WP_015687028.1; NC_017082.1.
DR   STRING; 335659.S23_45550; -.
DR   MEROPS; M38.982; -.
DR   EnsemblBacteria; BAL77749; BAL77749; S23_45550.
DR   KEGG; brs:S23_45550; -.
DR   PATRIC; fig|335659.3.peg.4564; -.
DR   KO; K01428; -.
DR   OrthoDB; 157757at2; -.
DR   BioCyc; BSP335659:S23_RS22205-MONOMER; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000007886; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000007886};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01953, ECO:0000256|PROSITE-
KW   ProRule:PRU00700, ECO:0000256|SAAS:SAAS00317631};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_01953, ECO:0000256|PROSITE-
KW   ProRule:PRU00700, ECO:0000256|SAAS:SAAS00321417};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01953,
KW   ECO:0000256|PIRSR:PIRSR611612-51, ECO:0000256|SAAS:SAAS00321440};
KW   Nickel {ECO:0000256|HAMAP-Rule:MF_01953,
KW   ECO:0000256|PIRSR:PIRSR611612-51, ECO:0000256|SAAS:SAAS00317628}.
FT   DOMAIN      134    568       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    320    320       Proton donor. {ECO:0000256|HAMAP-Rule:
FT                                MF_01953, ECO:0000256|PIRSR:PIRSR611612-
FT                                52, ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   METAL       139    139       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953,
FT                                ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       141    141       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953,
FT                                ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       217    217       Nickel 1; via carbamate group.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953,
FT                                ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       217    217       Nickel 2; via carbamate group.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953,
FT                                ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       246    246       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953,
FT                                ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       272    272       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|HAMAP-Rule:MF_01953,
FT                                ECO:0000256|PIRSR:PIRSR611612-51}.
FT   METAL       360    360       Nickel 1. {ECO:0000256|HAMAP-Rule:
FT                                MF_01953, ECO:0000256|PIRSR:PIRSR611612-
FT                                51}.
FT   BINDING     219    219       Substrate. {ECO:0000256|HAMAP-Rule:
FT                                MF_01953, ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     217    217       N6-carboxylysine. {ECO:0000256|HAMAP-
FT                                Rule:MF_01953, ECO:0000256|PIRSR:
FT                                PIRSR611612-50}.
SQ   SEQUENCE   568 AA;  60920 MW;  D948B24E8270FF82 CRC64;
     MAIQSRRAYG ELYGPTKGDL VRLADTSLLA EIEHDYTTYG HELLVGAGKN LRDGEGVNAY
     RTASHKILDV VIKNATIVDA VAGIVKADIG IRDGRIVGIG KAGNPDVMPD VHPDMVVGHT
     TAPIAGGPFI VTAGAVESHA HLISPEQSDH ALSGGTTTLV GNGSGPVFDV GSGAASTFAR
     FLQAIEFSPL NFALFGRAGS NPEAVEEAVA SGGMSVKIHE DFGAAPAVID QSLIAADRND
     FAVHLHTDSI NEYGFCEDTM AAVEGRTIHM YHVEGAGGGH APDLLKVVSW DNVIPSSTNP
     TNPYTSYGME EGVPMTMICH QLNYNAPEDV MFGEARVRAQ SMAAEDFLHD MGAISIFGTD
     TQGMGRLAEN VAKCWQLASV MKDRIGRLPE EKTARADNER IKRYIAKLTI NPAIAVGIDH
     TVGSIEPGKM ADLVLWPRAS FGIKPYMVIK NGFVVWAAMG DGNGSLGLSE PMIQKRMWGA
     LGAAPQRLGV NFMSKLAMEA DVSRKLGLSR DPVQIKNVRK LRKTDMVRNV AMPHVDVDPQ
     TFEVRADGKL LMCPPATKVP LARRYMLR
//
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