ID I0JXH3_METFB Unreviewed; 531 AA.
AC I0JXH3;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=DUF1957 domain-containing protein {ECO:0008006|Google:ProtNLM};
GN ORFNames=MFUM_270039 {ECO:0000313|EMBL:CCG91942.1};
OS Methylacidiphilum fumariolicum (strain SolV).
OC Bacteria; Verrucomicrobiota; Methylacidiphilae; Methylacidiphilales;
OC Methylacidiphilaceae; Methylacidiphilum (ex Ratnadevi et al. 2023).
OX NCBI_TaxID=1156937 {ECO:0000313|EMBL:CCG91942.1, ECO:0000313|Proteomes:UP000004837};
RN [1] {ECO:0000313|EMBL:CCG91942.1, ECO:0000313|Proteomes:UP000004837}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SolV {ECO:0000313|EMBL:CCG91942.1,
RC ECO:0000313|Proteomes:UP000004837};
RX PubMed=22740660; DOI=10.1128/JB.00501-12;
RA Khadem A.F., Wieczorek A.S., Pol A., Vuilleumier S., Harhangi H.R.,
RA Dunfield P.F., Kalyuzhnaya M.G., Murrell J.C., Francoijs K.-J.,
RA Stunnenberg H.G., Stein L.Y., DiSpirito A.A., Semrau J.D., Lajus A.,
RA Medigue C., Klotz M.G., Jetten M.S.M., Op den Camp H.J.M.;
RT "Draft Genome Sequence of the Volcano-Inhabiting Thermoacidophilic
RT Methanotroph Methylacidiphilum fumariolicum Strain SolV.";
RL J. Bacteriol. 194:3729-3730(2012).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCG91942.1}.
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DR EMBL; CAHT01000030; CCG91942.1; -; Genomic_DNA.
DR RefSeq; WP_009059273.1; NZ_CAHT01000030.1.
DR AlphaFoldDB; I0JXH3; -.
DR STRING; 1156937.GCA_000953475_00818; -.
DR eggNOG; COG1543; Bacteria.
DR InParanoid; I0JXH3; -.
DR OrthoDB; 9803279at2; -.
DR Proteomes; UP000004837; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Reference proteome {ECO:0000313|Proteomes:UP000004837}.
FT DOMAIN 10..402
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 430..530
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT ACT_SITE 194
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 357
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 246
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 263
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 410
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 470
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
SQ SEQUENCE 531 AA; 62308 MW; 3997EC09FE9CC778 CRC64;
MKSSPLGYLA LLLHAHLPFV RHPEDEEVLE EDWLFEAITE CYIPLLWMLE ELYQAAIRCK
LTLSLSPTLC SMLKDPLLIQ RYKRYLLKRI ELCSKEIERY AAADPERLLL AKFYKERFQN
VWKSFTEVYA EDILGQFKKY HEKSLLELVT SSATHGYLPL LKNPKQAVNA QIFVAIESFF
DSFQTYPKGF WLPECGYYPG IEFFLQSAEI RWFVLEAHGL LFSEPRPFLG LFSPIYTPSG
PAAFGRDSLS SKEVWSAQEG YPGDPVYREF YWDLGFESDL DYIRPYILPT GQRKFTGIKY
YRITGKTEQK ALYNPQKAQQ RAKEHAENFI MKREEAIKKA APLFPTFTKP ILLCPFDCEL
FGHWWFEGPL FLKELIKKAH QSEILELTTP LEYLHRYPTQ QICRPTYSSW GLEGYSKMWL
NETNDFIYQH LHEAAYLLVE LVRKYKNGNK QTVRSLKQAA RELLLAQASD WPFLISKSTA
KEYAFLRVIT HLNRFSELCE GIIQQKINKS LLEYCEYTDN LFPSLNLNYF S
//
