ID I0YSD3_COCSC Unreviewed; 674 AA.
AC I0YSD3;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN ORFNames=COCSUDRAFT_66819 {ECO:0000313|EMBL:EIE21302.1};
OS Coccomyxa subellipsoidea (strain C-169) (Green microalga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Trebouxiophyceae incertae sedis; Elliptochloris clade; Coccomyxa;
OC Coccomyxa subellipsoidea.
OX NCBI_TaxID=574566 {ECO:0000313|EMBL:EIE21302.1, ECO:0000313|Proteomes:UP000007264};
RN [1] {ECO:0000313|EMBL:EIE21302.1, ECO:0000313|Proteomes:UP000007264}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-169 {ECO:0000313|EMBL:EIE21302.1,
RC ECO:0000313|Proteomes:UP000007264};
RX PubMed=22630137; DOI=10.1186/gb-2012-13-5-r39;
RA Blanc G., Agarkova I., Grimwood J., Kuo A., Brueggeman A., Dunigan D.,
RA Gurnon J., Ladunga I., Lindquist E., Lucas S., Pangilinan J., Proschold T.,
RA Salamov A., Schmutz J., Weeks D., Yamada T., Claverie J.M., Grigoriev I.,
RA Van Etten J., Lomsadze A., Borodovsky M.;
RT "The genome of the polar eukaryotic microalga coccomyxa subellipsoidea
RT reveals traits of cold adaptation.";
RL Genome Biol. 13:R39-R39(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIE21302.1}.
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DR EMBL; AGSI01000012; EIE21302.1; -; Genomic_DNA.
DR RefSeq; XP_005645846.1; XM_005645789.1.
DR AlphaFoldDB; I0YSD3; -.
DR STRING; 574566.I0YSD3; -.
DR GeneID; 17039287; -.
DR KEGG; csl:COCSUDRAFT_66819; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000007264; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:EIE21302.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Reference proteome {ECO:0000313|Proteomes:UP000007264};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..674
FT /note="cellulase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003636348"
FT DOMAIN 87..538
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 625..674
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 626..647
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 648..662
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 674 AA; 75146 MW; 1F379868552EBA49 CRC64;
MLPRRSVAIA LIGCFVVLCV DHAAAVNKKV PSGTAVTAKQ ARLKKESAEK CDDSNAEHCY
DHTANVGIGP IKVSEKFPAA DQSTYDYSEV LHKSFLFYFQ QRSGKLPHQR LAWRTDSCAE
CKGPSGEDLS GGYYEAGGSY LKFSFPTAFT ITQLAWGVVE YRSGYEKVGE LQEALDAIKW
GTDYLLNCHV EPTKFVAMYG SSEVDFGYFG PPEEHLQWTQ EEREVTYITP EDPSSEICGE
VAAAFAASSL ALRDTHPEYA DKLVEHAKQM LEFGMEHPGS YMKSQLEGLK DHGKHYPSTN
YNDEMALGAV WLYFATGEDE YLQTATMYYD KLNSWKADNY AFDWDNKSPA LHVLLTQIYP
SEMMKYGVNA KQYFEEYLPG PKRTVFHTKK GLSWRNAWGV LRYSANNAFL AFVHSAQMKE
QGDEAYAATL FTYATQQINY MLGDSGRSFV VGFGKTPPST PFHKWSFNSY IDYPTRGQSY
EIQHDEFHNT DTPNKFLAYG ALVGGPNADD TFEDSRGWCC AAYNEVAVDY NAAFTGALAR
LVDYYTDMQP FSDCTLDLGW THSNATQAKR PNWPADDCYH ACCEGAARPT VRKEIASPTT
TKAVAKQAAK EAAVKESAKI VEATAEATEA KTEEQAKPTD GKAHAGGRGA ERKHRRSKDA
KGGHKDRRQA RIGL
//
