ID I0YV72_COCSC Unreviewed; 556 AA.
AC I0YV72;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 24-JAN-2024, entry version 48.
DE SubName: Full=Purine 5'-nucleotidase {ECO:0000313|EMBL:EIE22291.1};
GN ORFNames=COCSUDRAFT_29586 {ECO:0000313|EMBL:EIE22291.1};
OS Coccomyxa subellipsoidea (strain C-169) (Green microalga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Trebouxiophyceae incertae sedis; Elliptochloris clade; Coccomyxa;
OC Coccomyxa subellipsoidea.
OX NCBI_TaxID=574566 {ECO:0000313|EMBL:EIE22291.1, ECO:0000313|Proteomes:UP000007264};
RN [1] {ECO:0000313|EMBL:EIE22291.1, ECO:0000313|Proteomes:UP000007264}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-169 {ECO:0000313|EMBL:EIE22291.1,
RC ECO:0000313|Proteomes:UP000007264};
RX PubMed=22630137; DOI=10.1186/gb-2012-13-5-r39;
RA Blanc G., Agarkova I., Grimwood J., Kuo A., Brueggeman A., Dunigan D.,
RA Gurnon J., Ladunga I., Lindquist E., Lucas S., Pangilinan J., Proschold T.,
RA Salamov A., Schmutz J., Weeks D., Yamada T., Claverie J.M., Grigoriev I.,
RA Van Etten J., Lomsadze A., Borodovsky M.;
RT "The genome of the polar eukaryotic microalga coccomyxa subellipsoidea
RT reveals traits of cold adaptation.";
RL Genome Biol. 13:R39-R39(2012).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIE22291.1}.
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DR EMBL; AGSI01000010; EIE22291.1; -; Genomic_DNA.
DR RefSeq; XP_005646835.1; XM_005646778.1.
DR AlphaFoldDB; I0YV72; -.
DR GeneID; 17040277; -.
DR KEGG; csl:COCSUDRAFT_29586; -.
DR eggNOG; KOG2469; Eukaryota.
DR OrthoDB; 3626840at2759; -.
DR Proteomes; UP000007264; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF15; CYTOSOLIC PURINE 5'-NUCLEOTIDASE; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000007264}.
FT REGION 533..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 410..473
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 51
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 53
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 51
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 53
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 369
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 556 AA; 63866 MW; 9ADFC6726607EE91 CRC64;
MLPPPPPPVS EDYPAWWDEV WASPLDVRRR IFCNRSLNMA HIKAGFAIGF DMDYTLSQYR
PETFEVLAYN LTINKLIHNF DYPKELLDFE FDFRYMMRGL IIDKKRGNIL KMDRHKYVKV
AFHGFRKLTR EERLNTYAHA RERTEFDEPD YALIDTLFSL AEAHLFMQII ELMESKPGIM
PPNKTFADIY RDVRAAVDLC HRDGSLKRSV AENPAKYIHE DPRLPKLLEE LRASGRKLFL
ATNSLWDYTH VVMNFLLAGR TGADKNFEWL EYFDVVITGC GKPEFFIKKK ALFEVHTETG
MLCNTDGGTP MVPIGEEDLP APGFMSSAPG GLGEHEAGAQ CRVFQGGFYQ DLHKMLDISS
GGEVLYVGDH IYGDILRSKK TLGWRTMLVV PELETELEIQ AASVGVGCEL KTLRQQRDGL
EDQIQRLEAT IARSRAAGLL DGDPDEEDLE EVVANLREQR DKVRERHSAL LQEHHERFHK
VWGQLLKTGY QNSRFAHQVE RFACLYTSHV SNLCFYSPDK SYRGRMDHMA HEEEPAFVEP
PACPLPNPRR SQTADS
//
