ID I1C4D1_RHIO9 Unreviewed; 920 AA.
AC I1C4D1;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 24-JAN-2024, entry version 51.
DE RecName: Full=Cytochrome b5 heme-binding domain-containing protein {ECO:0000259|PROSITE:PS50255};
GN ORFNames=RO3G_08016 {ECO:0000313|EMBL:EIE83311.1};
OS Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL
OS 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar).
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC Mucoromycetes; Mucorales; Mucorineae; Rhizopodaceae; Rhizopus.
OX NCBI_TaxID=246409 {ECO:0000313|EMBL:EIE83311.1, ECO:0000313|Proteomes:UP000009138};
RN [1] {ECO:0000313|EMBL:EIE83311.1, ECO:0000313|Proteomes:UP000009138}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880
RC {ECO:0000313|Proteomes:UP000009138};
RX PubMed=19578406; DOI=10.1371/journal.pgen.1000549;
RA Ma L.-J., Ibrahim A.S., Skory C., Grabherr M.G., Burger G., Butler M.,
RA Elias M., Idnurm A., Lang B.F., Sone T., Abe A., Calvo S.E.,
RA Corrochano L.M., Engels R., Fu J., Hansberg W., Kim J.-M., Kodira C.D.,
RA Koehrsen M.J., Liu B., Miranda-Saavedra D., O'Leary S.,
RA Ortiz-Castellanos L., Poulter R., Rodriguez-Romero J., Ruiz-Herrera J.,
RA Shen Y.-Q., Zeng Q., Galagan J., Birren B.W., Cuomo C.A., Wickes B.L.;
RT "Genomic analysis of the basal lineage fungus Rhizopus oryzae reveals a
RT whole-genome duplication.";
RL PLoS Genet. 5:E1000549-E1000549(2009).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- SIMILARITY: Belongs to the acyl-CoA dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00009347}.
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DR EMBL; CH476737; EIE83311.1; -; Genomic_DNA.
DR AlphaFoldDB; I1C4D1; -.
DR STRING; 246409.I1C4D1; -.
DR VEuPathDB; FungiDB:RO3G_08016; -.
DR eggNOG; KOG0139; Eukaryota.
DR eggNOG; KOG0537; Eukaryota.
DR eggNOG; KOG1182; Eukaryota.
DR InParanoid; I1C4D1; -.
DR OrthoDB; 1474114at2759; -.
DR Proteomes; UP000009138; Unassembled WGS sequence.
DR GO; GO:0003863; F:3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity; IEA:UniProtKB-EC.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IEA:InterPro.
DR CDD; cd02000; TPP_E1_PDC_ADC_BCADC; 1.
DR Gene3D; 3.40.50.970; -; 1.
DR Gene3D; 1.10.540.10; Acyl-CoA dehydrogenase/oxidase, N-terminal domain; 1.
DR Gene3D; 2.40.110.10; Butyryl-CoA Dehydrogenase, subunit A, domain 2; 1.
DR Gene3D; 1.20.140.10; Butyryl-CoA Dehydrogenase, subunit A, domain 3; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR InterPro; IPR006091; Acyl-CoA_Oxase/DH_mid-dom.
DR InterPro; IPR046373; Acyl-CoA_Oxase/DH_mid-dom_sf.
DR InterPro; IPR036250; AcylCo_DH-like_C.
DR InterPro; IPR009075; AcylCo_DH/oxidase_C.
DR InterPro; IPR013786; AcylCoA_DH/ox_N.
DR InterPro; IPR037069; AcylCoA_DH/ox_N_sf.
DR InterPro; IPR009100; AcylCoA_DH/oxidase_NM_dom_sf.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR018506; Cyt_B5_heme-BS.
DR InterPro; IPR001017; DH_E1.
DR InterPro; IPR029061; THDP-binding.
DR PANTHER; PTHR43380; 2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43380:SF1; 2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL; 1.
DR Pfam; PF00441; Acyl-CoA_dh_1; 1.
DR Pfam; PF02770; Acyl-CoA_dh_M; 1.
DR Pfam; PF02771; Acyl-CoA_dh_N; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00676; E1_dh; 1.
DR PRINTS; PR00363; CYTOCHROMEB5.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF47203; Acyl-CoA dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF56645; Acyl-CoA dehydrogenase NM domain-like; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 1.
DR PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000009138}.
FT DOMAIN 2..78
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
SQ SEQUENCE 920 AA; 103596 MW; A7C0AF9473967920 CRC64;
MSKKFTIEEV AKHTQADDCW IIVHDKVYDI SKFLDDHPGG KKVLLKAAGT DATKQFEAFH
NPSVLTKVAA QYLIGDIGAE VEDDDDIPNA NPLQVGESYG DMVPFGDPMW YQDWYSPYYD
DSHRKVRKSV RDFVEREIMP YTHEWDEAKE LPREVYIKAA KAGLLASVVG HVEAEYIPYD
LPGNIKHEDF DAFHSLIVTD EISRCASGGV TWGLMGGLGI GLPPIIHFGS RYLKNKVIRD
CLSGEKHICL AITEPTGGSD VANLQTEAKD MGDHYLVNGE KKWITNGTFA DYFTVACRTG
EPGFNGLSFL LIERGMPGVI TRQMKCSGVW PSGTAYITFE DVKVPKENLI GKENKGFKYI
MQNFNSERIG IVIQANRFAR CCVEEALKYA HKRKTFGKRL VDHPVIRNKF ANMIRKVEAT
HAWLEILVYQ AKNMPTELQP IRLGGPIALC KAQSTRTFEF CAREAAQIFG GLAYTRGGQG
EKVERLYREV RAFAIPGGSE EIMLDLGVRQ ALFNNASVNV AEVANDGQNK STWSSADKFM
NEMKFIQQIE PLKAYRVMDH TGTVLNPEHD PNLSKEEVTK CYKSMLLLHT MDGILYDAQR
QGRISFYMTH YGEEAMIGSA AALLPEDVVF GQYREAFMLV YRGFTLDEFV NQCFSNELDY
GKGRQMPIHY GSKRLNFQTI SSPLGTQIPQ ASGAAYALKR SGANACTLCF FGEGAASEGD
FHAGLNMAAT LKCPVIFFCR NNGFAISTPS SEQYKGDGIA SRGIGYGIDT IRVDGNDIWA
IYNATKVARE IAVKEQRPVL IEAMTYRIGH HSTSDDSTKY RDRKEVEERA QLDNPITRLK
KYMEAKGWWS AQEEEDYRKK VKKEILASFS AAEKKKKPAL KHLFTDVYDE MTPNLVRQQN
ELNELIQKYP EYYNTDEFAS
//
