ID I1GZ80_BRADI Unreviewed; 493 AA.
AC I1GZ80;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=100844035 {ECO:0000313|EnsemblPlants:KQK18693};
GN ORFNames=BRADI_1g44110v3 {ECO:0000313|EMBL:KQK18693.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368 {ECO:0000313|EnsemblPlants:KQK18693};
RN [1] {ECO:0000313|EMBL:KQK18693.1, ECO:0000313|EnsemblPlants:KQK18693}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQK18693.1,
RC ECO:0000313|EnsemblPlants:KQK18693};
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2] {ECO:0000313|EMBL:KQK18693.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQK18693.1};
RG The International Brachypodium Initiative;
RA Lucas S., Harmon-Smith M., Lail K., Tice H., Grimwood J., Bruce D.,
RA Barry K., Shu S., Lindquist E., Wang M., Pitluck S., Vogel J.P.,
RA Garvin D.F., Mockler T.C., Schmutz J., Rokhsar D., Bevan M.W.;
RT "WGS assembly of Brachypodium distachyon.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:KQK18693}
RP IDENTIFICATION.
RC STRAIN=cv. Bd21 {ECO:0000313|EnsemblPlants:KQK18693};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM000880; KQK18693.1; -; Genomic_DNA.
DR RefSeq; XP_010227779.1; XM_010229477.2.
DR AlphaFoldDB; I1GZ80; -.
DR STRING; 15368.I1GZ80; -.
DR EnsemblPlants; KQK18693; KQK18693; BRADI_1g44110v3.
DR GeneID; 100844035; -.
DR Gramene; KQK18693; KQK18693; BRADI_1g44110v3.
DR KEGG; bdi:100844035; -.
DR eggNOG; ENOG502QR9R; Eukaryota.
DR HOGENOM; CLU_008926_1_2_1; -.
DR InParanoid; I1GZ80; -.
DR OMA; PERIGCK; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000008810; Chromosome 1.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF184; ENDOGLUCANASE 17; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000008810};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 25..493
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5013982624"
FT DOMAIN 28..484
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 410
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 462
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 471
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 493 AA; 53579 MW; F0DE58DAC811624A CRC64;
MASAAKAMAV VAATVLLLST AAMAQHNYAD ALHKSILFFE GQRSGRLPPD QRVRWRRDSA
LNDGATAGVD LTGGYYDAGD NVKFGFPMAF TATLMSWGLI DFGRSFGSHE GEARKAVRWA
TDYLMKATAT PNTVYVQVGD AFRDHSCWER PEDMDTPRTV YKVDPAHPGS DVAAETAAAL
AAGSIVFREA DPASSKRLLD RAVSVFAFAD KYRGAYSSSL HAAVCPCYCD YDGYQDELLW
GAAWLHKASR QRVYRDYIKK NEVALGASES INEFGWDNKH AGINVLISKE VLMGKDQFFQ
SFRVNADNFI CTLLPGISNH PQIQYSPGGL LFKVGNSNMQ HVTSLSFLLL AYSNYLSHAN
ARVACGGASS ASPVQLRRVA KRQVDYILGD NPLRMSYMVG YGARFPRRIH HRGSSLPSVS
AHPARIGCKA GAAYYGSSAP NPNLLVGAVV GGPSNTSDNF PDARAVFQQS EPTTYINAPL
LGLLAYFSVH PSI
//
