ID I1HMM3_BRADI Unreviewed; 602 AA.
AC I1HMM3;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Phosphoinositide phospholipase C {ECO:0000256|ARBA:ARBA00012368, ECO:0000256|RuleBase:RU361133};
DE EC=3.1.4.11 {ECO:0000256|ARBA:ARBA00012368, ECO:0000256|RuleBase:RU361133};
GN Name=100835224 {ECO:0000313|EnsemblPlants:KQK07876};
GN Synonyms=PI-PLC3 {ECO:0000313|EMBL:QSL97011.1};
GN ORFNames=BRADI_2g38170v3 {ECO:0000313|EMBL:KQK07876.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368 {ECO:0000313|EMBL:KQK07876.1};
RN [1] {ECO:0000313|EMBL:KQK07876.1, ECO:0000313|EnsemblPlants:KQK07876}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQK07876.1,
RC ECO:0000313|EnsemblPlants:KQK07876};
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2] {ECO:0000313|EMBL:KQK07876.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQK07876.1};
RG The International Brachypodium Initiative;
RA Lucas S., Harmon-Smith M., Lail K., Tice H., Grimwood J., Bruce D.,
RA Barry K., Shu S., Lindquist E., Wang M., Pitluck S., Vogel J.P.,
RA Garvin D.F., Mockler T.C., Schmutz J., Rokhsar D., Bevan M.W.;
RT "WGS assembly of Brachypodium distachyon.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:KQK07876}
RP IDENTIFICATION.
RC STRAIN=cv. Bd21 {ECO:0000313|EnsemblPlants:KQK07876};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
RN [4] {ECO:0000313|EMBL:QSL97011.1}
RP NUCLEOTIDE SEQUENCE.
RA Young J.E., Dvorak C., Murtaugh M.P., Wang X.;
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-
CC bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-
CC diacyl-sn-glycerol + H(+); Xref=Rhea:RHEA:33179, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17815, ChEBI:CHEBI:58456,
CC ChEBI:CHEBI:203600; EC=3.1.4.11;
CC Evidence={ECO:0000256|ARBA:ARBA00001195,
CC ECO:0000256|RuleBase:RU361133};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
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DR EMBL; CM000881; KQK07876.1; -; Genomic_DNA.
DR EMBL; CM000881; PNT71990.1; -; Genomic_DNA.
DR EMBL; MT247378; QSL97011.1; -; mRNA.
DR RefSeq; XP_003569051.1; XM_003569003.3.
DR AlphaFoldDB; I1HMM3; -.
DR STRING; 15368.I1HMM3; -.
DR EnsemblPlants; KQK07876; KQK07876; BRADI_2g38170v3.
DR EnsemblPlants; PNT71990; PNT71990; BRADI_2g38170v3.
DR GeneID; 100835224; -.
DR Gramene; KQK07876; KQK07876; BRADI_2g38170v3.
DR Gramene; PNT71990; PNT71990; BRADI_2g38170v3.
DR KEGG; bdi:100835224; -.
DR eggNOG; KOG0169; Eukaryota.
DR HOGENOM; CLU_002738_3_2_1; -.
DR InParanoid; I1HMM3; -.
DR OMA; MLHGSSM; -.
DR OrthoDB; 882863at2759; -.
DR Proteomes; UP000008810; Chromosome 2.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; IBA:GO_Central.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR GO; GO:0051209; P:release of sequestered calcium ion into cytosol; IBA:GO_Central.
DR CDD; cd00275; C2_PLC_like; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.20.20.190; Phosphatidylinositol (PI) phosphodiesterase; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR001192; PI-PLC_fam.
DR InterPro; IPR017946; PLC-like_Pdiesterase_TIM-brl.
DR InterPro; IPR000909; PLipase_C_PInositol-sp_X_dom.
DR InterPro; IPR001711; PLipase_C_Pinositol-sp_Y.
DR PANTHER; PTHR10336:SF36; 1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA PLC-3; 1.
DR PANTHER; PTHR10336; PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00388; PI-PLC-X; 1.
DR Pfam; PF00387; PI-PLC-Y; 1.
DR PRINTS; PR00390; PHPHLIPASEC.
DR SMART; SM00239; C2; 1.
DR SMART; SM00148; PLCXc; 1.
DR SMART; SM00149; PLCYc; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF51695; PLC-like phosphodiesterases; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50007; PIPLC_X_DOMAIN; 1.
DR PROSITE; PS50008; PIPLC_Y_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Hydrolase {ECO:0000256|RuleBase:RU361133};
KW Lipid degradation {ECO:0000256|RuleBase:RU361133};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361133};
KW Reference proteome {ECO:0000313|Proteomes:UP000008810};
KW Transducer {ECO:0000256|ARBA:ARBA00023224}.
FT DOMAIN 368..454
FT /note="PI-PLC Y-box"
FT /evidence="ECO:0000259|PROSITE:PS50008"
FT DOMAIN 449..584
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT REGION 269..329
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 273..289
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 602 AA; 67339 MW; 29E56E4E2A6FD344 CRC64;
MTTYRVCCFL RRFRPASSEP SAAIAAVFRS YASAASAGDV LGEEALKRFL REVQGEEGGS
AGVGDEDEDG GVEALVREIM AFAAQQKLLK KGGGGGGIAA EGFHRWLCSD ANAAIHPRRG
VHQDMGQPLS HYYIYTGHNS YLTGNQLSSG SSVAPIVKSL LDGVRVIELD LWPNAGKDDV
EVLHGRTWTS PVGLGECLDA VKEHAFVSSP YPVILTLEDH LTPHLQAKVA KMIKETFGDM
LHISQSETMA EFPSPDDLKG KIIISTKQPK EYLQTKSSKE DSQNGDKEEE ESVWGEEIPD
NKAQPAVARQ MSEPDNGQGV EEEDEMDKKV QPGVDGEYKR LIAIQLTRRK HDMNEDLKVD
PEKVTRMSLG EKAYEKATIT HGADIIRFTQ RNLLRIFPRT TRITSSNYNP LMGWRYGAQM
VAANMQGHGR KLWLTQGMFR ANGGCGYVKK PDFLMNSDPD KMFDPRSKLP VKTRLKVTLY
MGDGWRFDFH KTHFDKFSPP DFYARVGIAG VAADTRMEES KVVMDNWIPT WDHEFVFSLA
VPELALLRIE VHEADNHQKD DFAGQTCLPV WELRQGIRSV RLCARDGELL RSVKLLMRFE
FA
//
