ID I1HMP5_BRADI Unreviewed; 497 AA.
AC I1HMP5;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=100838588 {ECO:0000313|EnsemblPlants:PNT72010};
GN ORFNames=BRADI_2g38357v3 {ECO:0000313|EMBL:PNT72010.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368 {ECO:0000313|EnsemblPlants:PNT72010};
RN [1] {ECO:0000313|EMBL:PNT72010.1, ECO:0000313|EnsemblPlants:PNT72010}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bd21 {ECO:0000313|EMBL:PNT72010.1,
RC ECO:0000313|EnsemblPlants:PNT72010};
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2] {ECO:0000313|EMBL:PNT72010.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Bd21 {ECO:0000313|EMBL:PNT72010.1};
RG The International Brachypodium Initiative;
RA Lucas S., Harmon-Smith M., Lail K., Tice H., Grimwood J., Bruce D.,
RA Barry K., Shu S., Lindquist E., Wang M., Pitluck S., Vogel J.P.,
RA Garvin D.F., Mockler T.C., Schmutz J., Rokhsar D., Bevan M.W.;
RT "WGS assembly of Brachypodium distachyon.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:PNT72010}
RP IDENTIFICATION.
RC STRAIN=cv. Bd21 {ECO:0000313|EnsemblPlants:PNT72010};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM000881; PNT72010.1; -; Genomic_DNA.
DR RefSeq; XP_010231817.1; XM_010233515.1.
DR AlphaFoldDB; I1HMP5; -.
DR STRING; 15368.I1HMP5; -.
DR EnsemblPlants; PNT72010; PNT72010; BRADI_2g38357v3.
DR GeneID; 100838588; -.
DR Gramene; PNT72010; PNT72010; BRADI_2g38357v3.
DR KEGG; bdi:100838588; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_0_1; -.
DR InParanoid; I1HMP5; -.
DR OMA; YVVNATH; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000008810; Chromosome 2.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF125; ENDOGLUCANASE 12-RELATED; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000008810};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 27..497
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5005134955"
FT DOMAIN 30..491
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 418
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ SEQUENCE 497 AA; 52827 MW; 218BDF929215A604 CRC64;
MAIMARIVLL LALALVVFMA TPAASGAIDY AAALSKSLLY FEAQRSGRLP ATQRVQWRGH
SALKDGADHG VDLTGGYYDS GDNVKFGLPM AFTVTMLSWA VAEQRAGLER AAELGHALDA
VRWGADYLVR AHVAGNGGDG EKLFVQVGDG DSDHKCWERP EDMDTPRGAY EVNATHPGSD
VAAETAAALA AASVVFRDAH AAGGGNYAAT LLTHAKQLFE FAKNHRGLYH NSVPSAKIFY
PSGGDQDELL WAAAWLYIAT GEKNYERFIA DGGNGGVPPL FTWDYKYVGA QTLVAKLVLQ
GKLPDSGNYG ALKSNLEKYL CNIMQHGDGS SGKLSPGGML WVQPWDNQQC VTAAAFALVA
HADHLATAGG SLQCGGVKLP PAQLVSFARS QVDYILGKNP QKISYMVGYG DRYPEQVHHR
GASLPSIKAS PAKIGCSDGS GYFHTAKPNE NVIVGAIVGG PDANDRYNDS RGNYVQGETS
TYTVAPIVGV LARLVHS
//
