ID I1I0F1_BRADI Unreviewed; 946 AA.
AC I1I0F1;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 24-JAN-2024, entry version 55.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN Name=100843668 {ECO:0000313|EnsemblPlants:KQJ94835};
GN ORFNames=BRADI_3g13520v3 {ECO:0000313|EMBL:KQJ94835.1};
OS Brachypodium distachyon (Purple false brome) (Trachynia distachya).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Stipodae; Brachypodieae; Brachypodium.
OX NCBI_TaxID=15368 {ECO:0000313|EMBL:KQJ94835.1};
RN [1] {ECO:0000313|EMBL:KQJ94835.1, ECO:0000313|EnsemblPlants:KQJ94835}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQJ94835.1,
RC ECO:0000313|EnsemblPlants:KQJ94835};
RX PubMed=20148030; DOI=10.1038/nature08747;
RG International Brachypodium Initiative;
RT "Genome sequencing and analysis of the model grass Brachypodium
RT distachyon.";
RL Nature 463:763-768(2010).
RN [2] {ECO:0000313|EMBL:KQJ94835.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Bd21 {ECO:0000313|EMBL:KQJ94835.1};
RG The International Brachypodium Initiative;
RA Lucas S., Harmon-Smith M., Lail K., Tice H., Grimwood J., Bruce D.,
RA Barry K., Shu S., Lindquist E., Wang M., Pitluck S., Vogel J.P.,
RA Garvin D.F., Mockler T.C., Schmutz J., Rokhsar D., Bevan M.W.;
RT "WGS assembly of Brachypodium distachyon.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:KQJ94835}
RP IDENTIFICATION.
RC STRAIN=cv. Bd21 {ECO:0000313|EnsemblPlants:KQJ94835};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
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DR EMBL; CM000882; KQJ94835.1; -; Genomic_DNA.
DR EMBL; CM000882; PNT66516.1; -; Genomic_DNA.
DR RefSeq; XP_003571307.1; XM_003571259.3.
DR RefSeq; XP_014757147.1; XM_014901661.1.
DR AlphaFoldDB; I1I0F1; -.
DR STRING; 15368.I1I0F1; -.
DR EnsemblPlants; KQJ94835; KQJ94835; BRADI_3g13520v3.
DR EnsemblPlants; PNT66516; PNT66516; BRADI_3g13520v3.
DR GeneID; 100843668; -.
DR Gramene; KQJ94835; KQJ94835; BRADI_3g13520v3.
DR Gramene; PNT66516; PNT66516; BRADI_3g13520v3.
DR KEGG; bdi:100843668; -.
DR eggNOG; KOG2119; Eukaryota.
DR HOGENOM; CLU_006322_1_0_1; -.
DR InParanoid; I1I0F1; -.
DR OMA; NDPWHEL; -.
DR OrthoDB; 360472at2759; -.
DR Proteomes; UP000008810; Chromosome 3.
DR ExpressionAtlas; I1I0F1; baseline.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF27; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000008810}.
FT DOMAIN 85..405
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 509..874
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
SQ SEQUENCE 946 AA; 104373 MW; 77905F089E8661C7 CRC64;
MGSSELEQTK AHSHIDCAQP PERTWQRKFD DEGKEIAMFS FTMNDIMTVA PLIVKMMRIL
VEERAKGKAS VYDPFKKWMS NCYRGVPLGT LGAGSIGRSY RGYFQHFQLF PGIYEQKPIL
ANQFSAFVSR PGGKSYSTVL SAPPAEVLKG IDKASIGSWD WNLKEKNCSY HGLFPRSWTV
YDGEPDPEIK ITCRQISPFI PRNYKESSFP VAVFTFTVQN SGSTPADVTL LFTWANSVGG
KSELTGNHSN STMRARDGVH GVLLRHSTAE GHPPVTFAIA SQDTGDVRVT CCPSFSMGPS
SKSGEPTAKE MWEEIKKHGS FGDAAAGTGR PSRPGSSVGA AVAAATTVTA GCTREVSFAL
SWSCPVVKFP AGRTYHRRHT KFVGLDRDAA AEQLAHHALL EHMEWERQIE AWQRPVLQDK
SLPDWYPVAL FNELYYLNAG GTIWTDGMPS KKTSLVSSSS GTMEPFSLAA FHPDPNATSS
STAADDILLA MARAEEHLLP AAEDEKGVGK FLYLEGMEYH MYNTYDVHFY ASFALLSLFP
DLELSLQRDF AAAVLRHDPR LMYTLDGKFV PRKVLGAVPH DIGLNDPWHE LNAYMIHDPS
RWKDLNPKFV LQVYRDVAAT GDLDFARSAW PSVYVALAYM DQFDRDRDGM VENEGRPDQT
YDLWSVSGVS AYTGGLWVAA LRAATAMAAL VGDLPAEAVF LERYNRANKV YDSELWTGDY
FRYDNSGGGN SESVMADQLA GQWYARACGL EPVVGRDKAR RALAAVLEHN VMQVQGGGVG
AVNGARLPEH GGGVDESSTQ SKEVWTGTTY AVAAAMIGEG MREEGFTAAK GAYGAGWGED
GYGYAFQMPE SWTADGAGGY RSLHYMRPLA VWAMQWALSP PTPVLPELER MAAKSKATEE
EVELAKEKFE KVASLLRLPE EEKKKEKGYL RAFYQILRQI LLPAAY
//
