ID I1M8V2_SOYBN Unreviewed; 430 AA.
AC I1M8V2;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 2.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN Name=100803878 {ECO:0000313|EnsemblPlants:KRG88755};
GN ORFNames=GLYMA_U029700 {ECO:0000313|EMBL:KRG88755.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EnsemblPlants:KRG88755};
RN [1] {ECO:0000313|EMBL:KRG88755.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRG88755.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRG88755}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRG88755};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRG88755.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRG88755.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|RuleBase:RU369093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369093};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
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DR EMBL; KZ847140; KRG88755.1; -; Genomic_DNA.
DR RefSeq; XP_003545347.2; XM_003545299.3.
DR AlphaFoldDB; I1M8V2; -.
DR SMR; I1M8V2; -.
DR PaxDb; 3847-GLYMA14G09980-1; -.
DR EnsemblPlants; KRG88755; KRG88755; GLYMA_U029700.
DR GeneID; 100803878; -.
DR Gramene; KRG88755; KRG88755; GLYMA_U029700.
DR KEGG; gmx:100803878; -.
DR eggNOG; ENOG502QTEJ; Eukaryota.
DR HOGENOM; CLU_006348_1_2_1; -.
DR InParanoid; I1M8V2; -.
DR OMA; MDCRSTI; -.
DR OrthoDB; 5489991at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000008827; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849:SF6; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Transferase {ECO:0000256|RuleBase:RU369093};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU369093}.
FT DOMAIN 44..118
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
SQ SEQUENCE 430 AA; 47566 MW; 0839900F70130C8D CRC64;
MILEEGCAKM QFFQPSKERE DENAKIEEAG VDLKVMIEEM ESIDVPSVFI CPISLEPMQD
PVTLCTGQTY DRSNILKWFS LGHKTCPTTM QELWDDVVTP NSTLSHLMLT WFSQKYLALK
KKLKDVQGRA LEILNMLKKV KGQARVRALQ DLRQLVASHV NARKALEENG GVALVFNFLG
PFTSHAVGSE AIGIIVCLDL SSEVKRSLMH PAKVSLLVDI MNEGTIETKM NCAKLIEMLL
VEGNNETVSS LSLLVGLLRL VRDKKHPNGV VSIGLILLKK AIICSHESVR SSLISLGAIP
LLIELLPSLN NECLEKALYI LEVLSTLPEG RMALKECPNI IPNVVKLLMR VSERCTQFAL
SILWAIYKLA PEECASKAVE AGLAAKLLLV IQSGCNPVLK QMSSEFLKMC SLNYSTSILV
SKCMLTTTIQ
//
