ID I1MFX5_SOYBN Unreviewed; 618 AA.
AC I1MFX5;
DT 13-JUN-2012, integrated into UniProtKB/TrEMBL.
DT 13-JUN-2012, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=100793289 {ECO:0000313|EnsemblPlants:KRH11699};
GN ORFNames=GLYMA_15G124600 {ECO:0000313|EMBL:KRH11699.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EMBL:KRH11699.1};
RN [1] {ECO:0000313|EMBL:KRH11699.1, ECO:0000313|EnsemblPlants:KRH11699}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH11699};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH11699.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH11699}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH11699};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH11699.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH11699.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; CM000848; KRH11699.1; -; Genomic_DNA.
DR RefSeq; XP_003546229.1; XM_003546181.3.
DR AlphaFoldDB; I1MFX5; -.
DR SMR; I1MFX5; -.
DR STRING; 3847.I1MFX5; -.
DR PaxDb; 3847-GLYMA15G13080-1; -.
DR EnsemblPlants; KRH11699; KRH11699; GLYMA_15G124600.
DR GeneID; 100793289; -.
DR Gramene; KRH11699; KRH11699; GLYMA_15G124600.
DR KEGG; gmx:100793289; -.
DR eggNOG; ENOG502QUUK; Eukaryota.
DR HOGENOM; CLU_008926_1_3_1; -.
DR InParanoid; I1MFX5; -.
DR OMA; GNSGMRD; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000008827; Chromosome 15.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF36; ENDOGLUCANASE 25; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059}; Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 74..93
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 109..582
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 512
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 560
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 569
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 618 AA; 68346 MW; 224275462307BAC3 CRC64;
MYSRDSWGGP LEINATDSAT EDERSRNLQD VDRAALSRPL DETQQSWLLG PGEQKKKKYV
DLGCIIVSRK IFKWTLGTLL FAAFLAGLIA LIVNTVPRHR HKHPPPDNYT LALHKALMFF
NGQRSGKLPK HNNVSWRGNS GMQDGKSSDH SAAIKDLVGG YYDAGDAIKF NFPASFSITM
LSWSVIEYSA KYEAAGELDH VKEIIKWGTD YFLKSFNSTA DTITTLAAQV GLGDTSGGDS
PNDHYCWMRP EDMDYDRPVT ECHSCSDLAA EMAAALASAS IVFKDNKAYS KKLVHGATTL
FKFSRDQRGR YSAGSSEASI FYNSTSYWDE YVWGGAWMYF ATGNSSYLKL ATAPGLAKHA
GAFWGGPDYG VLSWDNKLAG AQVLLSRLRL FLSPGYPYEE ILKTFHNQTS IIMCSYLPVF
TSFNRTKGGL IQLNHGRPQP LQYVVNAAFL AALYSDYLDA ADTPGWYCGP NFFSTDVLRN
FAKTQIDYIL GNNPRKMSYV VGFGNHYPKH VHHRGASIPK NKIKYSCKGG WKWRDTSKPN
PNTIVGAMVA GPDKHDGFHD VRTNYNYTEP TLAGNAGLVA ALVALSGDKS TSIDKNTIFS
AVPPMFPTPP PPPAPWKP
//
