ID I3D352_9ARCH Unreviewed; 382 AA.
AC I3D352;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Malic enzyme, NAD binding domain protein {ECO:0000313|EMBL:EIJ66145.1};
GN ORFNames=BD31_I1611 {ECO:0000313|EMBL:EIJ66145.1};
OS Candidatus Nitrosopumilus salaria BD31.
OC Archaea; Nitrososphaerota; Nitrososphaeria; Nitrosopumilales;
OC Nitrosopumilaceae; Nitrosopumilus.
OX NCBI_TaxID=859350 {ECO:0000313|EMBL:EIJ66145.1, ECO:0000313|Proteomes:UP000003423};
RN [1] {ECO:0000313|EMBL:EIJ66145.1, ECO:0000313|Proteomes:UP000003423}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BD31 {ECO:0000313|EMBL:EIJ66145.1,
RC ECO:0000313|Proteomes:UP000003423};
RX PubMed=22461555; DOI=10.1128/JB.00013-12;
RA Mosier A.C., Allen E.E., Kim M., Ferriera S., Francis C.A.;
RT "Genome sequence of "Candidatus Nitrosopumilus salaria" BD31, an ammonia-
RT oxidizing archaeon from the San Francisco Bay estuary.";
RL J. Bacteriol. 194:2121-2122(2012).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: Belongs to the malic enzymes family.
CC {ECO:0000256|ARBA:ARBA00008785}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIJ66145.1}.
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DR EMBL; AEXL02000083; EIJ66145.1; -; Genomic_DNA.
DR RefSeq; WP_008298854.1; NZ_AEXL02000083.1.
DR AlphaFoldDB; I3D352; -.
DR PATRIC; fig|859350.6.peg.816; -.
DR OrthoDB; 45556at2157; -.
DR Proteomes; UP000003423; Unassembled WGS sequence.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR015884; Malic_enzyme_CS.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR001891; Malic_OxRdtase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR PIRSF; PIRSF000106; ME; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00331; MALIC_ENZYMES; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 13..146
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 159..378
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
SQ SEQUENCE 382 AA; 42014 MW; 6356A8293F42E559 CRC64;
MSKKAILLHR KLQGKIFIES KISNLSSKNL QLIYTPGVAS VCKEIMKNPE SKYTLTSKGN
NIAIITDGTR LLGLGNVGAN VSLPVMEGKS AIYRAYGKIN AFPICLNTTD KKKIVDIVEA
IEPMFGAINL EDIESPKVLE IYKELEKRLE IPVFHDDGQG TAIVTLAGLI NALKIVKKNL
SEIKIIVAGS GSAGYFIGKL LNFAGCKNII MLDSKGVIYK NRKNNMTKYK REIAKITNPK
EKGQLSEVIK NADVFIGVSG IEDLFKLEAI KTMNKNPIVF ALTNPYPEIN PHDAKKGGAK
IVATGSFLYK NTINNAIVFP YLMRSILDLK IKKITMEVLY ATSLAVAKTI SDQKLSPNNI
IPSIENKSLQ KNITKSLKNL KS
//
