ID I3DIA7_9PAST Unreviewed; 629 AA.
AC I3DIA7;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2012, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE SubName: Full=Signal peptide peptidase SppA, 67K type {ECO:0000313|EMBL:EIJ71450.1};
DE EC=3.4.-.- {ECO:0000313|EMBL:EIJ71450.1};
GN Name=sppA {ECO:0000313|EMBL:EIJ71450.1};
GN ORFNames=HMPREF1052_0176 {ECO:0000313|EMBL:EIJ71450.1};
OS Pasteurella bettyae CCUG 2042.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Pasteurella.
OX NCBI_TaxID=1095749 {ECO:0000313|EMBL:EIJ71450.1, ECO:0000313|Proteomes:UP000006457};
RN [1] {ECO:0000313|EMBL:EIJ71450.1, ECO:0000313|Proteomes:UP000006457}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 2042 {ECO:0000313|EMBL:EIJ71450.1,
RC ECO:0000313|Proteomes:UP000006457};
RA Harkins D.M., Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA Nelson K.E.;
RL Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase S49 family.
CC {ECO:0000256|ARBA:ARBA00008683}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIJ71450.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJSX01000007; EIJ71450.1; -; Genomic_DNA.
DR RefSeq; WP_005758962.1; NZ_AJSX01000007.1.
DR AlphaFoldDB; I3DIA7; -.
DR PATRIC; fig|1095749.3.peg.409; -.
DR eggNOG; COG0616; Bacteria.
DR OrthoDB; 9764363at2; -.
DR Proteomes; UP000006457; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR CDD; cd07018; S49_SppA_67K_type; 1.
DR CDD; cd07023; S49_Sppa_N_C; 1.
DR Gene3D; 6.20.330.10; -; 1.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR004634; Pept_S49_pIV.
DR InterPro; IPR004635; Pept_S49_SppA.
DR InterPro; IPR002142; Peptidase_S49.
DR InterPro; IPR047217; S49_SppA_67K_type_N.
DR InterPro; IPR047272; S49_SppA_C.
DR NCBIfam; TIGR00705; SppA_67K; 1.
DR NCBIfam; TIGR00706; SppA_dom; 1.
DR PANTHER; PTHR33209; PROTEASE 4; 1.
DR PANTHER; PTHR33209:SF1; SERINE PROTEASE SPPA, CHLOROPLASTIC; 1.
DR Pfam; PF01343; Peptidase_S49; 2.
DR PIRSF; PIRSF001217; Protease_4_SppA; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:EIJ71450.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000006457};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..44
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 137..290
FT /note="Peptidase S49"
FT /evidence="ECO:0000259|Pfam:PF01343"
FT DOMAIN 398..545
FT /note="Peptidase S49"
FT /evidence="ECO:0000259|Pfam:PF01343"
FT ACT_SITE 205
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
FT ACT_SITE 414
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
SQ SEQUENCE 629 AA; 70436 MW; A0183FE06E06389E CRC64;
MQSICKLFKW CWRGLTFIRD CVMNLFFLFF IFLFMSIISL TSALKEKTVL EGEQGALLVN
LYGYLADERG DNLNWQSAYQ EINGEKVPHQ YSTFDVFYAI ESAAQDERIK GLVLDLNHLE
GADIPALTYL GKAIQDFQST GKSVIAYADN YTQAQYLLAS YADEIYLNKI GEVGIEGLSA
QNLYFKSMLE KLEVTPHVFR VGTYKSAVEP FLRDNMSEEA RDNLKQWLYP MWSNYQDIIA
QNRQIDKGMV LPKASQYLTE LKKLNGNNTD YAKQRKLITE TVTRLDLSKK LIALFGENKE
KQPKMVEFET YLASLPDRMN GESEGLISSE NKIAVVNVEG TIIDGDSTEQ GVGGDTIAKL
LRQAYDDESI KAVVLRVNSP GGSAFASEII RQETENLQAI GKPVVVSMGN MAASGGYWIS
STADYIVADK NTLTGSIGIF AMFPTLEKTI KKAGISADGL ATSELVSPSG FTALTPELNE
SLQLQIDHGY DRFLSVVSQG RHLSKEDVDK IAQGRVWLGQ DAYNNKLVDE IGDFDTAVAQ
VEKLLNDKLP EDKKGADYSI EWLSDDRGLF GSLMRDFKRG AESFFSSSFY SMLGLPKQLN
QVKNQLSIWT QFNDPKGQYL YCLNCSQIK
//
