ID I3DZ50_BACMM Unreviewed; 574 AA.
AC I3DZ50;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2012, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=yloA {ECO:0000313|EMBL:AIE59594.1};
GN Synonyms=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=BMMGA3_05830 {ECO:0000313|EMBL:AIE59594.1};
OS Bacillus methanolicus (strain MGA3 / ATCC 53907).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=796606 {ECO:0000313|EMBL:AIE59594.1, ECO:0000313|Proteomes:UP000027602};
RN [1] {ECO:0000313|EMBL:AIE59594.1, ECO:0000313|Proteomes:UP000027602}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGA3 / ATCC 53907 {ECO:0000313|Proteomes:UP000027602};
RX PubMed=25758049;
RA Irla M., Neshat A., Brautaset T., Ruckert C., Kalinowski J., Wendisch V.F.;
RT "Transcriptome analysis of thermophilic methylotrophic Bacillus
RT methanolicus MGA3 using RNA-sequencing provides detailed insights into its
RT previously uncharted transcriptional landscape.";
RL BMC Genomics 16:73-73(2015).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
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DR EMBL; CP007739; AIE59594.1; -; Genomic_DNA.
DR RefSeq; WP_003348940.1; NZ_CP007739.1.
DR AlphaFoldDB; I3DZ50; -.
DR STRING; 796606.BMMGA3_05830; -.
DR KEGG; bmet:BMMGA3_05830; -.
DR eggNOG; COG1293; Bacteria.
DR HOGENOM; CLU_022481_2_1_9; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000027602; Chromosome.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.8.50; -; 1.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000027602};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 453..541
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
SQ SEQUENCE 574 AA; 65868 MW; C99081A1E14067F7 CRC64;
MSFDGLFTRA MVNELSQTIN GGRINKIHQP SKNEIIITVR ANGRNHKLLL SVHPSYARVQ
LTNEPYENPT EPPMFCMLLR KHLEGFILEN IYQEGLDRII VFDIKGRNEI GDVSYKQLIV
EIMGRHSNII LIDKASNTIL DSIKHVSFAV NTHRAVLPGY KYIAPPEQNK FDPFLADEQE
ILKRLDFNAG KLDKQLVDQF SGISPLLAKE IVFQSGMANR ITLPKTFLTY MEKIKKNEYI
PAITYGSSKE SFYLYPLEHI KGEHKIFSSL SAMLDRYYFG KAERDRVKQQ AHDLERFILN
EKEKNIKKIE KLQLELKEAK NAEKYQLYGE LLTANLYAVQ KGMKEIEVMN YYDENSGTIV
IPLDPQKSPS ENAQKYFTKY QKAKNALTVI QKQIEKAKVE VSYFDSLLQQ IEAASTKDIM
EIREELIEGG YLRERHKQQN KKQLSTKPIL DKYLSSDGTE ILVGKNNKQN DYLTNKLAAR
DEIWLHAKDI PGSHVVIRSK TPTEKTILEA ANLAAYFSKA RNSSSVPVDY TLVRYVKKPS
GAKPGFVIYE NQQTIYVTPD EELVLALKQN TEKV
//
