ID I3JEV9_ORENI Unreviewed; 571 AA.
AC I3JEV9; I3JEV8;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=TNF receptor-associated factor {ECO:0000256|PIRNR:PIRNR015614};
DE EC=2.3.2.27 {ECO:0000256|PIRNR:PIRNR015614};
GN Name=traf6 {ECO:0000313|Ensembl:ENSONIP00000007400.2};
GN Synonyms=TRAF6 {ECO:0000313|EMBL:AWX49579.1};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000007400.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AWX49579.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Spleen {ECO:0000313|EMBL:AWX49579.1};
RX PubMed=29933111;
RA Wang Z., Huang Y., Li Y., Wang B., Lu Y., Xia L., Tang J., Jian J.;
RT "Biological characterization, expression, and functional analysis of tumor
RT necrosis factor receptor-associated factor 6 in Nile tilapia (Oreochromis
RT niloticus).";
RL Fish Shellfish Immunol. 80:497-504(2018).
RN [3] {ECO:0000313|Ensembl:ENSONIP00000007400.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|PIRNR:PIRNR015614};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000256|ARBA:ARBA00004544}. Lipid droplet
CC {ECO:0000256|ARBA:ARBA00004502}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the TNF receptor-associated factor family. A
CC subfamily. {ECO:0000256|ARBA:ARBA00006608}.
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DR EMBL; MG148336; AWX49579.1; -; mRNA.
DR RefSeq; XP_003450846.1; XM_003450798.4.
DR RefSeq; XP_005455784.1; XM_005455727.2.
DR RefSeq; XP_005455785.1; XM_005455728.3.
DR Ensembl; ENSONIT00000007405.2; ENSONIP00000007400.2; ENSONIG00000040460.1.
DR GeneID; 100703551; -.
DR KEGG; onl:100703551; -.
DR CTD; 7189; -.
DR GeneTree; ENSGT00940000155426; -.
DR HOGENOM; CLU_021061_5_0_1; -.
DR OMA; SNIMHLG; -.
DR OrthoDB; 2913784at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000005207; Linkage group LG1.
DR GO; GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:UniProt.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR GO; GO:0043122; P:regulation of canonical NF-kappaB signal transduction; IEA:InterPro.
DR CDD; cd03776; MATH_TRAF6; 1.
DR CDD; cd16643; mRING-HC-C3HC3D_TRAF6; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 3.
DR InterPro; IPR002083; MATH/TRAF_dom.
DR InterPro; IPR012227; TNF_rcpt-assoc_TRAF_met.
DR InterPro; IPR008974; TRAF-like.
DR InterPro; IPR037309; TRAF6_MATH.
DR InterPro; IPR027139; TRAF6_RING-HC.
DR InterPro; IPR041310; TRAF6_Z2.
DR InterPro; IPR049342; TRAF_MEP1_MATH_dom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR InterPro; IPR001293; Znf_TRAF.
DR PANTHER; PTHR10131; TNF RECEPTOR ASSOCIATED FACTOR; 1.
DR PANTHER; PTHR10131:SF94; TNF RECEPTOR-ASSOCIATED FACTOR 6; 1.
DR Pfam; PF21355; TRAF-mep_MATH; 1.
DR Pfam; PF18048; TRAF6_Z2; 1.
DR Pfam; PF13923; zf-C3HC4_2; 1.
DR Pfam; PF02176; zf-TRAF; 1.
DR PIRSF; PIRSF015614; TRAF; 1.
DR SMART; SM00061; MATH; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF49599; TRAF domain-like; 3.
DR PROSITE; PS50144; MATH; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
DR PROSITE; PS50145; ZF_TRAF; 2.
PE 2: Evidence at transcript level;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR015614};
KW Lipid droplet {ECO:0000256|ARBA:ARBA00022677};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR015614}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Receptor {ECO:0000313|EMBL:AWX49579.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRNR:PIRNR015614};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00207}.
FT DOMAIN 77..116
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 154..202
FT /note="TRAF-type"
FT /evidence="ECO:0000259|PROSITE:PS50145"
FT DOMAIN 211..265
FT /note="TRAF-type"
FT /evidence="ECO:0000259|PROSITE:PS50145"
FT DOMAIN 397..544
FT /note="MATH"
FT /evidence="ECO:0000259|PROSITE:PS50144"
FT ZN_FING 154..202
FT /note="TRAF-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00207"
FT ZN_FING 211..265
FT /note="TRAF-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00207"
SQ SEQUENCE 571 AA; 64088 MW; 54EA1C00A98F6C72 CRC64;
MACCESNKGS LEEDSYDGAV GGVLSGCALA MLEKEQESIL SPSENPSAFM SSSSLQAAPL
QGYDVEFDPP LESKYECPIC LMALRNAIQT RCGHRFCKSC IEKSIRDTGQ RCPVDNEMLS
EDQLFPDNFA KREILSLTVR CPNSGCAEKM ELRHLEDHLV HCEFATVPCP QCQQSVKKSH
LEEHITVQCQ KRPMSCPDCV ACFLYEEREL HQQQCPFASV KCQYCELELV RDQMESHCDT
DCPKAPIACN FSIFGCKERM QRHNLAQHMQ EFTQMHMRYM AEFLRGLSLN GTTPKPLRTL
GPSLSSEDQG AAASVPACSG SRGAAALSCN SSCAPGPSSE EMQQRLREMD GRLVKQDHQL
RELIILKDTQ AGQLAELRRR VSMLEDTVKE LEAQHCNGIF IWRLKGFSVH LRNQEAGQPV
VEHSPGFYTG RPGYKLCLRL HLQTPSAPRC SSYISLFVHT MQGAFDGQLT WPFQGTIRLA
ILDQGPEGQH HMEAMETKPD LQAFQKPTIQ RNPKGFGYVT FMHLSQLSQR AYVKDDTLLI
RCEVTPRFDN ILHREGPAVQ PRGPEAFASR D
//
