ID I3JW42_ORENI Unreviewed; 630 AA.
AC I3JW42;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=Galactocerebrosidase {ECO:0000256|ARBA:ARBA00019657};
DE EC=3.2.1.46 {ECO:0000256|ARBA:ARBA00012657};
DE AltName: Full=Galactosylceramidase {ECO:0000256|ARBA:ARBA00033098};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000013087.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000013087.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a D-galactosylceramide + H2O = an N-acyl-sphingoid base + D-
CC galactose; Xref=Rhea:RHEA:43412, ChEBI:CHEBI:4139, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:36498, ChEBI:CHEBI:83273;
CC Evidence={ECO:0000256|ARBA:ARBA00023982};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43413;
CC Evidence={ECO:0000256|ARBA:ARBA00023982};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-galactosyl-(1<->1)-sphing-4-enine + H2O = D-galactose +
CC sphing-4-enine; Xref=Rhea:RHEA:43908, ChEBI:CHEBI:4139,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:57756, ChEBI:CHEBI:57934;
CC Evidence={ECO:0000256|ARBA:ARBA00023423};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43909;
CC Evidence={ECO:0000256|ARBA:ARBA00023423};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 59 family.
CC {ECO:0000256|ARBA:ARBA00005637}.
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DR AlphaFoldDB; I3JW42; -.
DR Ensembl; ENSONIT00000013097.2; ENSONIP00000013087.2; ENSONIG00000010407.2.
DR eggNOG; ENOG502QQ1Q; Eukaryota.
DR GeneTree; ENSGT00390000003303; -.
DR TreeFam; TF312985; -.
DR Proteomes; UP000005207; Linkage group LG19.
DR GO; GO:0004336; F:galactosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006683; P:galactosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR049162; GH59_C.
DR InterPro; IPR049161; GH59_cat.
DR InterPro; IPR001286; Glyco_hydro_59.
DR InterPro; IPR035394; Glyco_hydro_59_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR15172; GALACTOCEREBROSIDASE; 1.
DR PANTHER; PTHR15172:SF1; GALACTOCEREBROSIDASE; 1.
DR Pfam; PF02057; Glyco_hydro_59; 1.
DR Pfam; PF21708; Glyco_hydro_59_C; 1.
DR Pfam; PF17387; Glyco_hydro_59M; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Sphingolipid metabolism {ECO:0000256|ARBA:ARBA00022919}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..630
FT /note="Galactocerebrosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5025543907"
FT DOMAIN 40..331
FT /note="Glycosyl hydrolase family 59 catalytic"
FT /evidence="ECO:0000259|Pfam:PF02057"
FT DOMAIN 339..396
FT /note="Glycosyl hydrolase family 59 central"
FT /evidence="ECO:0000259|Pfam:PF17387"
FT DOMAIN 455..627
FT /note="Glycosyl hydrolase family 59 C-terminal lectin"
FT /evidence="ECO:0000259|Pfam:PF21708"
FT ACT_SITE 183
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601286-50"
FT ACT_SITE 256
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR601286-50"
SQ SEQUENCE 630 AA; 69540 MW; 94188ACD3D40FA62 CRC64;
ALRMDCRESA LLVSVLFLAL DLCSAQNYVL SDDGGLGRVF DGIGGLSGGG ATSRLLVSYA
EPYRSQILDF LFKPNFGASL HILKVEIGGD AQTTDGTEPS HMHYENDENY YRGYEWWLMK
EAKKRNPNII LIGLPWAFPG WVGHGKNWPY DYPDVTATYV VNWILGAKQY HDLDIQYVGV
STESMSLSPK VLRNMLDKVG LPGVGIIAAD GDWSVANSMI LDPYLKNSVD VIGAHYPGTT
TITEALKTQK KLWSSEDYST FNNEVGGGCW ARILNQNYVN GQMTATISWN LVASYYEDLP
FGRDGLMTAE EPWSGNYVVE SPIWITAHTT QFTQPGWTYL QTVGHLAQGG SYIALTDGKG
NLTVVIETMT HNHSVCIRPP LPPFNVTSQN ATFQLKDGLF TLNLAEDEVY TLTTISTGSK
GSYPDPPPSA PFPKVYKDDF DVRNPPFSEA PYFADQTGVF EYYINLTDPG PHVFTLRQVL
TERPITWATD ADQTISVIGD YYWQNLTVTC DVFMEKMNAG GVFIAARVDK GGQSIRSAKG
VFFWVFADGT YKVTNDLVGE TVLAEGQSGT EVYGWYTLSL TVKGQYASGS LNGYPLWKNA
VVLTPKAGWA AIGTRSFELA QFDNFALVAE
//
