ID I3JZZ9_ORENI Unreviewed; 462 AA.
AC I3JZZ9;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=N-acetylglucosamine-6-sulfatase {ECO:0000256|PIRNR:PIRNR036666};
DE EC=3.1.6.14 {ECO:0000256|PIRNR:PIRNR036666};
DE AltName: Full=Glucosamine-6-sulfatase {ECO:0000256|PIRNR:PIRNR036666};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000014444.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000014444.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of the 6-sulfate groups of the N-acetyl-D-
CC glucosamine 6-sulfate units of heparan sulfate and keratan sulfate.;
CC EC=3.1.6.14; Evidence={ECO:0000256|PIRNR:PIRNR036666};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913,
CC ECO:0000256|PIRNR:PIRNR036666};
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|PIRNR:PIRNR036666}.
CC -!- PTM: The conversion to 3-oxoalanine (also known as C-formylglycine,
CC FGly), of a serine or cysteine residue in prokaryotes and of a cysteine
CC residue in eukaryotes, is critical for catalytic activity.
CC {ECO:0000256|PIRSR:PIRSR036666-50}.
CC -!- SIMILARITY: Belongs to the sulfatase family.
CC {ECO:0000256|ARBA:ARBA00008779, ECO:0000256|PIRNR:PIRNR036666}.
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DR AlphaFoldDB; I3JZZ9; -.
DR STRING; 8128.ENSONIP00000059716; -.
DR Ensembl; ENSONIT00000014455.2; ENSONIP00000014444.2; ENSONIG00000011477.2.
DR eggNOG; KOG3731; Eukaryota.
DR GeneTree; ENSGT00940000165439; -.
DR HOGENOM; CLU_006332_4_1_1; -.
DR TreeFam; TF313545; -.
DR Proteomes; UP000005207; Linkage group LG7.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008449; F:N-acetylglucosamine-6-sulfatase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030203; P:glycosaminoglycan metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd16147; G6S; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR012251; GlcNAc_6-SO4ase.
DR InterPro; IPR024607; Sulfatase_CS.
DR InterPro; IPR000917; Sulfatase_N.
DR PANTHER; PTHR43108:SF9; N-ACETYLGLUCOSAMINE-6-SULFATASE; 1.
DR PANTHER; PTHR43108; N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER; 1.
DR Pfam; PF00884; Sulfatase; 1.
DR PIRSF; PIRSF036666; G6S; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
DR PROSITE; PS00523; SULFATASE_1; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRNR:PIRNR036666};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR036666};
KW Lysosome {ECO:0000256|PIRNR:PIRNR036666};
KW Metal-binding {ECO:0000256|PIRNR:PIRNR036666};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 10..314
FT /note="Sulfatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00884"
FT MOD_RES 25
FT /note="3-oxoalanine (Cys)"
FT /evidence="ECO:0000256|PIRSR:PIRSR036666-50"
SQ SEQUENCE 462 AA; 52339 MW; 545ADB7610917D4A CRC64;
MKKAKAYIGD EGATFVNSFT VTPLCCPSRS SILTGRYPHN HEVRNNSLNG NCSSLQWQKG
PEAEAFPTYL NKLKYQTFFS GKYLNQYGSK AAGSVGHIPP GWDQWHALVG NSQYYNYTLS
VNGKEEKHGD SYEDDYLTDL ILNRSLHFLD DRSPQHPFFL MLSPPACHSP WTAAPQYQKE
FKDVQAPRDG SFNKPGKDKH WLLRQPLNPM PNSSVTFLDN AYRKRWQTLL SVDDMVEKLV
KKLDSIKELN NTYIFYTSDN GYHTGQFSLP IDKRQLYEFD IRIPLMVRGP GIKPKQAPVL
NIDLAPTILD IAGVNLSSVN VDGQSFLAQM APSLRNGTAR PYFLVEYTGE GQPTSDPTCP
KLGPGVSQCF PDCVCEDAYN NTYACVRTLD SKMNMQYCEF ADSESFVEVY NLTSDPHQLE
NIVKKVDPSI LQIMNQRLIK LQSCVSDSLY FSFYESKFLP SF
//
