ID I3K151_ORENI Unreviewed; 548 AA.
AC I3K151;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 57.
DE SubName: Full=Beta,beta-carotene 9',10'-oxygenase {ECO:0000313|Ensembl:ENSONIP00000014846.2};
GN Name=LOC100708996 {ECO:0000313|Ensembl:ENSONIP00000014846.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000014846.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000014846.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787, ECO:0000256|RuleBase:RU003799}.
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DR AlphaFoldDB; I3K151; -.
DR Ensembl; ENSONIT00000014858.2; ENSONIP00000014846.2; ENSONIG00000011793.2.
DR eggNOG; KOG1285; Eukaryota.
DR GeneTree; ENSGT00950000182913; -.
DR HOGENOM; CLU_016472_1_1_1; -.
DR TreeFam; TF314019; -.
DR Proteomes; UP000005207; Linkage group LG12.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543:SF107; B,B-CAROTENE-9',10'-DIOXYGENASE-RELATED; 1.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207}.
FT BINDING 189
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 253
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 324
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 541
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 548 AA; 62556 MW; 6E58FC3B1254B4E9 CRC64;
MLPTSAYDQQ RCPQASGLEC LAPLVRSAEE TPDPIPTTIK GTIPPWINGS FLRNGPGKFE
FGTDRYTHWF DGMAMMHRFH IREGNVTYSS RFLRSDSFVE NSEKNRIVVS EFGTLAMPDP
CKNIFARFLS RFQTPKPTDN ANVNFVKYKG DYYVSTETNY MRRVNPQSLE TKEKVDWSQY
IAVNSASAHP HYDRDGSTYN MGNSYSRSGF FYNIIRVPPP EEKAAMEDSA DLSGAKVICS
IPAADPRKPS YFHSFVMSEN YIVFVEQPIK IDLLKFMLYK IQGKSFSKVM TWEPQHATIF
HLVNRHTGKK SEVKYYTGPM FTLHQINAFE DSGFLVMDMC CGDDGELIGE FTLENLRRNS
GEEIDEFYNS LCRNLPRRYV LPLTVDEQTP ADENLITLHN CKATARMTKP GEVYMTHEEL
HNDELLQFGG LEFPQINYGQ FNGRPYRYFY SCGFGHVFSD SLLKMDVHTK ELKAWRYPGL
YPSEPVFVAS PKATEEDDGV VLSVIITPRE EKSTFLLVLD AKTFSELGRA EVPVNIPYGT
HGVFNEMG
//
