ID I3K8E1_ORENI Unreviewed; 1116 AA.
AC I3K8E1;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 2.
DT 27-MAR-2024, entry version 62.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSONIP00000017386.2};
GN Name=SLC12A2 {ECO:0000313|Ensembl:ENSONIP00000017386.2};
OS Oreochromis niloticus (Nile tilapia) (Tilapia nilotica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Oreochromini; Oreochromis.
OX NCBI_TaxID=8128 {ECO:0000313|Ensembl:ENSONIP00000017386.2, ECO:0000313|Proteomes:UP000005207};
RN [1] {ECO:0000313|Proteomes:UP000005207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Broad Institute Genome Assembly Team;
RG Broad Institute Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K.;
RT "The Genome Sequence of Oreochromis niloticus (Nile Tilapia).";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSONIP00000017386.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR AlphaFoldDB; I3K8E1; -.
DR Ensembl; ENSONIT00000017401.2; ENSONIP00000017386.2; ENSONIG00000013827.2.
DR eggNOG; KOG2083; Eukaryota.
DR GeneTree; ENSGT00940000155742; -.
DR HOGENOM; CLU_001883_0_0_1; -.
DR TreeFam; TF313191; -.
DR Proteomes; UP000005207; Linkage group LG12.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF105; SOLUTE CARRIER FAMILY 12 MEMBER 2-LIKE; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000005207};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 242..272
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 333..352
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 361..380
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 412..434
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 446..465
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 519..543
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 579..599
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 605..622
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 634..652
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 126..177
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 215..716
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 725..1116
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 23..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 98..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 882..907
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..127
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 885..907
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1116 AA; 122261 MW; CD640176996513F1 CRC64;
MLARDDNKSR CFFPGNRLSL GASLTEMSGQ KPSLRSDDSA QSRFQVDVVM EASSAAPGPA
APSAPASTDG SRPPEESKGR FRVVGFIDPG AMDIGLPPDV SHEPDTAKSD SVSLHSTGTG
HTHISDSHSN TYYMRTFGHN TIDAVPNIDF YRQTAAPFGE KLTRPSLSEL HDELDKEPFE
DGLANGEEPS AAEEAAALAA KEAKGGTVKF GWVKGVLIRC MLNIWGVMLF IRMSWIVGQA
GIGLTIAIIL MATVVTTITG LSTSAIATNG FVRGGGAYYL ISRSLGPEFG GSIGLIFAFA
NAVAVAMYVV GFAETVVELL NDVDALMTDE LNDIRIVGTL TIILLLGISV AGMEWEAKAQ
IVLLVILLAA IVNFFFGSFM PSESKEPKGF FGYHTAILLE NFGPEFRDGE TFFSVFAIFF
PAATGILAGA NISGDLTDPQ SAIPKGTLLA ILITGLTYVA VAISAGSCIV RDATGDQNDT
VSPTVNCTDA ACTLGYDFSI CKEGGCQFGL MNDFQVMSLV SAFGPLITAG IFSATLSSAL
ASLVSAPKVF QALCKDNIYP GLGMFAKGYG RNNEPLRGYV LTFCIGLAFI LIADLNIIAP
IISNFFLASY ALINFSVFHA SLASSPGWRP SFKYYNMWVS LVGAILCCVV MFVINWWAAL
VTLLIVLALY IYVSYKKPDV NWGSSTQALI YNQALTHCLN LTGVEDHVKN FRPQCLVLAG
YPNARPALLQ LVNSFTKNVS LMVCSHVRTV SRRSNFRELY QDYARCQRYL NKKRIKAFYA
PVFSDNLRHG AQLLLQAVGL GRLKPNTLVM GFKNNWSDGN MRDVENYINT IHDAFDLLFG
VVILRLQEGL DISHIQGQDE LLSSHEKPPV ITKDVLISVI PAKDSDSDSC PSKTTSNQSK
TKSPLSLTDQ RLLESSQQFK KKQGKGTIDV WWLFDDGGLT LLIPYLLTNR SKWGDCRIRV
FIGGKINRID HDRRAMATLL SRFRIDFSDI NVLGDINTKP KKHNKLTFKE LIEPYRLKED
DMEQEAAERL KAQEPWRITD NELELYKAKT NRQIRLNELL KEHSSTAKLI VMSMPLARKG
TVSSALYMCW LETLSKGLPP LLLVRGNHQS VLTFYS
//
