ID   I3L5B8_PIG              Unreviewed;       756 AA.
AC   I3L5B8;
DT   11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT   11-DEC-2019, sequence version 3.
DT   27-MAR-2024, entry version 54.
DE   SubName: Full=GRAM domain containing 1B {ECO:0000313|Ensembl:ENSSSCP00000019217.3};
GN   Name=GRAMD1B {ECO:0000313|Ensembl:ENSSSCP00000019217.3,
GN   ECO:0000313|VGNC:VGNC:88656};
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823 {ECO:0000313|Ensembl:ENSSSCP00000019217.3, ECO:0000313|Proteomes:UP000008227};
RN   [1] {ECO:0000313|Ensembl:ENSSSCP00000019217.3, ECO:0000313|Proteomes:UP000008227}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Duroc {ECO:0000313|Ensembl:ENSSSCP00000019217.3,
RC   ECO:0000313|Proteomes:UP000008227};
RG   Porcine genome sequencing project;
RL   Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSSSCP00000019217.3}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC       Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}.
CC       Endoplasmic reticulum membrane {ECO:0000256|ARBA:ARBA00004389}; Single-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004389}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004167}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; I3L5B8; -.
DR   PeptideAtlas; I3L5B8; -.
DR   Ensembl; ENSSSCT00000032190.4; ENSSSCP00000019217.3; ENSSSCG00000015144.5.
DR   VGNC; VGNC:88656; GRAMD1B.
DR   eggNOG; KOG1032; Eukaryota.
DR   GeneTree; ENSGT00940000156649; -.
DR   TreeFam; TF327695; -.
DR   Proteomes; UP000008227; Chromosome 9.
DR   Bgee; ENSSSCG00000015144; Expressed in testis and 43 other cell types or tissues.
DR   ExpressionAtlas; I3L5B8; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0044232; C:organelle membrane contact site; IEA:UniProt.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd13220; PH-GRAM_GRAMDC; 1.
DR   Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR   InterPro; IPR004182; GRAM.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR031968; VASt.
DR   PANTHER; PTHR23319:SF3; PROTEIN ASTER-B; 1.
DR   PANTHER; PTHR23319; UNCHARACTERIZED; 1.
DR   Pfam; PF02893; GRAM; 1.
DR   Pfam; PF16016; VASt; 1.
DR   SMART; SM00568; GRAM; 1.
DR   PROSITE; PS51778; VAST; 1.
PE   1: Evidence at protein level;
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:I3L5B8};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008227};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        652..672
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          401..572
FT                   /note="VASt"
FT                   /evidence="ECO:0000259|PROSITE:PS51778"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          282..330
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          572..596
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        9..26
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        287..325
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   756 AA;  86968 MW;  4B52080358E5229C CRC64;
     MLGTQLKGRK GGQQGSPKTL CQAGKGRSSA FSPGKPICLH PVRTVGKYRS LRSRVPVGGG
     AVGSKMESLT ESGVLWSLLL EQDSQPLQWY LRKLADVPAG TELCYWPGSE EIPAVLSPTY
     KQRNEDFRKL FKQLPDSERL IVDYSCALQR DILLQGRLYL SENWICFYSN IFRWETLLTV
     RLKDICSMTK EKTARLIPNA IQVCTDSEKH FFTSFGARDR TYMMMFRLWQ NALLEKPLCP
     KELWHFVHQC YGNELGLTSD DEDYVPPDDD VNTMGYCEEI PVEENEVNDS SSKSSIETKP
     DASPQLPKKS ITNSTLTSTG SSEAPVSFDG LPLEEEVLEG DGSLEKELAI DNIIGEKIEI
     IAPVTSPSLD FNDNEDIPTE LSDSSDTHDE GEVQAFYEDL SGRQYVNEVF NFSVDKLYDL
     LFTDSPFQRD FMEQRRFSDI IFHPWKKEEN GNQSRVILYT ITLTNPLAPK TATVRETQTM
     YKASQESECY VIDAEVLTHD VPYHDYFYTI NRYTLTRVAR NKSRLRVSTE LRYRKQPWGL
     VKTFIEKNFW SGLEDYFRHL ESELTKTEST YLAEMHRQSP KEKASKPPTV RRRKRPHAHL
     RVPHLEEVMS PVTTPTDEDV GHRIKHVAGS TQTRHIPEDT PSGFHLQSVS KLLLVISCVL
     VLLVILNVML FYKLWMLEYT TQTLTAWQGL RLQERLPQSQ TEWAQLLESQ QKYHDTELQK
     WREIIKSSVM LLDQVRYSMI QVQLGTYCFL VGTWRV
//