ID I3LW76_ICTTR Unreviewed; 318 AA.
AC I3LW76;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 11-JUL-2012, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=Protein-tyrosine sulfotransferase {ECO:0000256|RuleBase:RU365018};
DE EC=2.8.2.20 {ECO:0000256|RuleBase:RU365018};
GN Name=TPST2 {ECO:0000313|Ensembl:ENSSTOP00000000210.2};
OS Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus
OS tridecemlineatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Sciuromorpha; Sciuridae;
OC Xerinae; Marmotini; Ictidomys.
OX NCBI_TaxID=43179 {ECO:0000313|Ensembl:ENSSTOP00000000210.2, ECO:0000313|Proteomes:UP000005215};
RN [1] {ECO:0000313|Proteomes:UP000005215}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Assembly & Analysis Group;
RG Computational R&D Group;
RG and Sequencing Platform;
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lindblad-Toh K.;
RT "The Draft Genome of Spermophilus tridecemlineatus.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSSTOP00000000210.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the O-sulfation of tyrosine residues within acidic
CC motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as
CC cosubstrate. {ECO:0000256|ARBA:ARBA00003886,
CC ECO:0000256|RuleBase:RU365018}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3'-phosphoadenylyl sulfate + L-tyrosyl-[protein] = adenosine
CC 3',5'-bisphosphate + H(+) + O-sulfo-L-tyrosine-[protein];
CC Xref=Rhea:RHEA:16801, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:11688,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:58339,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:65286; EC=2.8.2.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000411,
CC ECO:0000256|RuleBase:RU365018};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000256|ARBA:ARBA00004323}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004323}. Membrane
CC {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane protein
CC {ECO:0000256|ARBA:ARBA00004606}.
CC -!- SIMILARITY: Belongs to the protein sulfotransferase family.
CC {ECO:0000256|ARBA:ARBA00009988, ECO:0000256|RuleBase:RU365018}.
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DR EMBL; AGTP01087802; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01087803; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01087804; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01087805; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; I3LW76; -.
DR STRING; 43179.ENSSTOP00000000210; -.
DR Ensembl; ENSSTOT00000000237.3; ENSSTOP00000000210.2; ENSSTOG00000000236.3.
DR eggNOG; KOG3988; Eukaryota.
DR GeneTree; ENSGT00390000006030; -.
DR HOGENOM; CLU_046916_0_1_1; -.
DR InParanoid; I3LW76; -.
DR OMA; WECQQIL; -.
DR TreeFam; TF312910; -.
DR Proteomes; UP000005215; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:Ensembl.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR GO; GO:0008476; F:protein-tyrosine sulfotransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR026634; TPST-like.
DR PANTHER; PTHR12788; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR PANTHER; PTHR12788:SF6; PROTEIN-TYROSINE SULFOTRANSFERASE 2; 1.
DR Pfam; PF13469; Sulfotransfer_3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Reference proteome {ECO:0000313|Proteomes:UP000005215};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365018};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
SQ SEQUENCE 318 AA; 35745 MW; EB3EE96880B0D12D CRC64;
VEYRYGRAMP LIFVGGVPRS GTTLMRAMLD AHPEVRCGEE TRIIPRVLAM RQSWSKSGRE
KLRLDEAGVT DEVLDAAMQA FILEVIAKHG EPARVLCNKD PFTLKSSVYL SRLFPNSKFL
LMVRDGRASV HSMITRKVTI AGFDLSSYRD CLTKWNKAIE VMYSQCMEVG RDKCLPVYYE
QLVLHPRRSL KLILDFLGIA WSDAVLHHED LIGKPGGVSL SKIERSTDQV IKPVNLEALS
KWTGHIPRDV VRDMARIAPM LARLGYDPYA NPPNYGDPDP IVINNTQRVL KGDYKTPANL
KGYFQVNQNS TSSHLGSS
//
