ID I3M2F4_ICTTR Unreviewed; 1125 AA.
AC I3M2F4;
DT 11-JUL-2012, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 2.
DT 27-MAR-2024, entry version 71.
DE SubName: Full=MCF.2 cell line derived transforming sequence like {ECO:0000313|Ensembl:ENSSTOP00000003154.3};
GN Name=MCF2L {ECO:0000313|Ensembl:ENSSTOP00000003154.3};
OS Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus
OS tridecemlineatus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Sciuromorpha; Sciuridae;
OC Xerinae; Marmotini; Ictidomys.
OX NCBI_TaxID=43179 {ECO:0000313|Ensembl:ENSSTOP00000003154.3, ECO:0000313|Proteomes:UP000005215};
RN [1] {ECO:0000313|Proteomes:UP000005215}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Assembly & Analysis Group;
RG Computational R&D Group;
RG and Sequencing Platform;
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lindblad-Toh K.;
RT "The Draft Genome of Spermophilus tridecemlineatus.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSSTOP00000003154.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; AGTP01003923; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003924; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003925; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003926; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003927; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003928; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003929; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003930; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003931; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGTP01003932; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; I3M2F4; -.
DR Ensembl; ENSSTOT00000003522.3; ENSSTOP00000003154.3; ENSSTOG00000003498.3.
DR GeneTree; ENSGT00940000157874; -.
DR HOGENOM; CLU_007130_1_0_1; -.
DR TreeFam; TF318080; -.
DR Proteomes; UP000005215; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR CDD; cd01227; PH_Dbs; 1.
DR CDD; cd00160; RhoGEF; 1.
DR CDD; cd00170; SEC14; 1.
DR CDD; cd00176; SPEC; 1.
DR Gene3D; 1.20.58.60; -; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR Gene3D; 2.30.30.40; SH3 Domains; 1.
DR InterPro; IPR001251; CRAL-TRIO_dom.
DR InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR035534; DBS_PH.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR001331; GDS_CDC24_CS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR22826:SF115; GUANINE NUCLEOTIDE EXCHANGE FACTOR DBS; 1.
DR PANTHER; PTHR22826; RHO GUANINE EXCHANGE FACTOR-RELATED; 1.
DR Pfam; PF13716; CRAL_TRIO_2; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF00018; SH3_1; 1.
DR Pfam; PF00435; Spectrin; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00516; SEC14; 1.
DR SMART; SM00326; SH3; 1.
DR SMART; SM00150; SPEC; 1.
DR SUPFAM; SSF52087; CRAL/TRIO domain; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF50044; SH3-domain; 1.
DR SUPFAM; SSF46966; Spectrin repeat; 1.
DR PROSITE; PS50191; CRAL_TRIO; 1.
DR PROSITE; PS00741; DH_1; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50002; SH3; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000005215};
KW SH3 domain {ECO:0000256|ARBA:ARBA00022443, ECO:0000256|PROSITE-
KW ProRule:PRU00192}.
FT DOMAIN 22..194
FT /note="CRAL-TRIO"
FT /evidence="ECO:0000259|PROSITE:PS50191"
FT DOMAIN 602..782
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 800..916
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 1025..1086
FT /note="SH3"
FT /evidence="ECO:0000259|PROSITE:PS50002"
FT REGION 232..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..596
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 926..1026
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1092..1125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..596
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 926..952
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1092..1117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1125 AA; 126669 MW; E396C1D14AF34E16 CRC64;
MRFWLRNEEM ALEEMVQRLN AVSRHTDEIM HQDIVPLCAA DIQEQLKRRF AYLSGGRGQD
GSPVITFPDY PAFSEIPDKE FQSVMTYLTS IPSLQDAGIG FILVIDRRQD KWTSVKASVL
RIAASFPANL QLVLVLRPTG FFQRTLSEIA FKFNRDDFKM KVPVIMLSSV PELHGYIDKS
QLTEDLGGTL DYCHSRWLCH RTAIESFALM VKQTAQMLQS FGTELAETEL PNDVQSTSSV
LSTHTEKKDK AKEDLQLAQK EGQRLLESLR EPLAESTAHS VNQDQLDSQA TVQRLLAQLN
ETEAAFHEFW AKHQQKLEQC LQLRHFEQGF REVKATLDTA SQKIATFTDI GNSLAHVEHL
LRSLASFEEK SSVAVERARA LAQQGQQLIE NKHYAVDSIH PKCEELQHLC HQFATEIGSR
RALLSKSQEL HRLLETSMKW CDEGIYLLAS QPVDKCQSQD GAEAALQEIE KFLETGEENK
IQELSKIYKE YETILNPDLL DHVQKVFQKQ ESMQEMFHRR QASLKKLAAK QTRPVQPVAP
RPEALTKSPC PSPGSWRGSE NSGPEGSALR RGPYRRAKSE MSENRQGRDS STGDEEEGLA
ILRRHVMNEL LDTERAYVEE LLSVLEGYAA EMENPLMTHL ISTGLHNKKD VLFGNMEEIY
HFHNRVFLRE LERCTDCPEL VGRCFLERME DFQIYEKYCQ NKPRSESLWR QCSTCPFFQE
CQKKLDHKLS LDSYLLKPVQ RITKYQLLLK EMLKYSRNCE GAEDLQEALS SILGILKAVN
DSMHLIAITG YDGNLSDLGK LLMQGSFSVW TDHKKGHTKV KELARFKPMQ RHLFLHEKAV
LFCKKREENG EGYEKAPSYS YKQSLNMTAV GITENVKGDA KKFEIWYNAR EEVYIVQAPT
PEIKAAWVNE IRKVLTSQLQ ACREASQHRT LEQSHSLPLP APSSTSPSGG NSRHVKKQEE
RKTDPLSLEG YVSSSLPKPP EKGKGWSKTS HSLEVPEDEG GWSSAEEPIN SSDAEEDGGV
GPKKLVPGKY TVVLDDEKGG PDTLAMRSGD VVEVVEEGAE GLWYVRDLSS GQEGWVPAGS
LSALLGTSSS AQCLSSSESS PGSAVLSNSS SCSEGCPAPF SDLQG
//
