ID I3TJC6_TISMK Unreviewed; 606 AA.
AC I3TJC6;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=Glutathione import ATP-binding protein GsiA {ECO:0000256|ARBA:ARBA00041187};
DE EC=7.4.2.10 {ECO:0000256|ARBA:ARBA00039050};
GN Name=gsiA {ECO:0000313|EMBL:AFK52864.1};
GN OrderedLocusNames=TMO_1025 {ECO:0000313|EMBL:AFK52864.1};
OS Tistrella mobilis (strain KA081020-065).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
OC Geminicoccaceae; Tistrella.
OX NCBI_TaxID=1110502 {ECO:0000313|EMBL:AFK52864.1, ECO:0000313|Proteomes:UP000005258};
RN [1] {ECO:0000313|EMBL:AFK52864.1, ECO:0000313|Proteomes:UP000005258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KA081020-065 {ECO:0000313|EMBL:AFK52864.1,
RC ECO:0000313|Proteomes:UP000005258};
RX PubMed=22458477; DOI=10.1021/ja301735a;
RA Xu Y., Kersten R.D., Nam S.J., Lu L., Al-Suwailem A.M., Zheng H.,
RA Fenical W., Dorrestein P.C., Moore B.S., Qian P.Y.;
RT "Bacterial biosynthesis and maturation of the didemnin anti-cancer
RT agents.";
RL J. Am. Chem. Soc. 134:8625-8632(2012).
CC -!- FUNCTION: Part of the ABC transporter complex GsiABCD involved in
CC glutathione import. Responsible for energy coupling to the transport
CC system. {ECO:0000256|ARBA:ARBA00037530}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glutathione(out) + H2O = ADP + glutathione(in) + H(+) +
CC phosphate; Xref=Rhea:RHEA:29791, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57925, ChEBI:CHEBI:456216; EC=7.4.2.10;
CC Evidence={ECO:0000256|ARBA:ARBA00036581};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (GsiA),
CC two transmembrane proteins (GsiC and GsiD) and a solute-binding protein
CC (GsiB). {ECO:0000256|ARBA:ARBA00011469}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004417}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004417}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Glutathione
CC importer (TC 3.A.1.5.11) family. {ECO:0000256|ARBA:ARBA00038416}.
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DR EMBL; CP003236; AFK52864.1; -; Genomic_DNA.
DR RefSeq; WP_014744543.1; NC_017956.1.
DR AlphaFoldDB; I3TJC6; -.
DR STRING; 1110502.TMO_1025; -.
DR KEGG; tmo:TMO_1025; -.
DR PATRIC; fig|1110502.3.peg.1059; -.
DR eggNOG; COG4172; Bacteria.
DR HOGENOM; CLU_000604_86_2_5; -.
DR Proteomes; UP000005258; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0015833; P:peptide transport; IEA:InterPro.
DR CDD; cd03257; ABC_NikE_OppD_transporters; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013563; Oligopep_ABC_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43776:SF15; GLUTATHIONE IMPORT ATP-BINDING PROTEIN GSIA; 1.
DR PANTHER; PTHR43776; TRANSPORT ATP-BINDING PROTEIN; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR Pfam; PF08352; oligo_HPY; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000313|EMBL:AFK52864.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000005258};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 14..264
FT /note="ABC transporter"
FT /evidence="ECO:0000259|PROSITE:PS50893"
FT DOMAIN 310..557
FT /note="ABC transporter"
FT /evidence="ECO:0000259|PROSITE:PS50893"
FT REGION 281..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 606 AA; 64551 MW; BE3F7E135E39F255 CRC64;
MTETASPPAP APVLEIEGLG VAFRGPAGWT SVVRNISLAV GPGGTVAVVG ESGSGKSVTA
MAAMRLLPAA TSRVTGSVRL EGQELTTLSE PDMCRVRGGR MSMIFQEPMT SLNPVRTIGF
QIAEALMLHR GLDRRAADAE VVRLLDRVRI PAAASRLGDH PHNFSGGMRQ RVMIAMALAC
RPKLLIADEP TTALDVTIQA QILGLIRELQ AEDGMSVLFI THDLGVVAEI ADRVVVMCRG
ALIETAGTAR IFDAPQQPYT RALLAAVPRL GAMRSIDRPT PFDAIDPESG AATPAAPLAG
PPDHAATPAL EIRGLTTRYL TGGGLFARRR PAVHAVENVS FTVAPGETLA LVGESGCGKS
TIGRTIMGLT RPHAGEVLVD GRPLDGLARR DAVRRVQMVF QDPFSSLNPR HSVGTAIAAP
LEINGIARGA AARARVAELL ALVGLDPQMA GRRPQEFSGG QRQRLCIARA LALEPSVLIA
DEAVSALDVS IKAQVVNLML ELQARLGLAY LFISHDMGVV ERVAHRVAVM YLGEIVEIGP
RAQVLYDPRH PYTRRLLDAV PVADPAARFR RRDIAPAEIA SPLRPAGWQP PVRRYDEVAP
GHFVMA
//
