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Database: UniProt
Entry: I3TTB8_TISMK
LinkDB: I3TTB8_TISMK
Original site: I3TTB8_TISMK 
ID   I3TTB8_TISMK            Unreviewed;      5215 AA.
AC   I3TTB8;
DT   05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT   06-MAR-2013, sequence version 2.
DT   12-SEP-2018, entry version 41.
DE   SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:AFK56006.2};
GN   OrderedLocusNames=TMO_a0603 {ECO:0000313|EMBL:AFK56006.2};
OS   Tistrella mobilis (strain KA081020-065).
OG   Plasmid pTM1 {ECO:0000313|EMBL:AFK56006.2,
OG   ECO:0000313|Proteomes:UP000005258}.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Tistrella.
OX   NCBI_TaxID=1110502 {ECO:0000313|EMBL:AFK56006.2, ECO:0000313|Proteomes:UP000005258};
RN   [1] {ECO:0000313|EMBL:AFK56006.2, ECO:0000313|Proteomes:UP000005258}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KA081020-065 {ECO:0000313|Proteomes:UP000005258};
RC   PLASMID=Plasmid pTM1 {ECO:0000313|Proteomes:UP000005258};
RX   PubMed=22458477; DOI=10.1021/ja301735a;
RA   Xu Y., Kersten R.D., Nam S.J., Lu L., Al-Suwailem A.M., Zheng H.,
RA   Fenical W., Dorrestein P.C., Moore B.S., Qian P.Y.;
RT   "Bacterial biosynthesis and maturation of the didemnin anti-cancer
RT   agents.";
RL   J. Am. Chem. Soc. 134:8625-8632(2012).
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DR   EMBL; CP003237; AFK56006.2; -; Genomic_DNA.
DR   RefSeq; WP_014747683.1; NC_017957.2.
DR   ProteinModelPortal; I3TTB8; -.
DR   EnsemblBacteria; AFK56006; AFK56006; TMO_a0603.
DR   KEGG; tmo:TMO_a0603; -.
DR   OrthoDB; POG091H088I; -.
DR   BioCyc; TMOB1110502:G1H5I-4201-MONOMER; -.
DR   Proteomes; UP000005258; Plasmid pTM1.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0008152; P:metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.1200.10; -; 6.
DR   Gene3D; 3.30.559.10; -; 8.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR010071; AA_adenyl_domain.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   InterPro; IPR032821; KAsynt_C_assoc.
DR   InterPro; IPR018201; Ketoacyl_synth_AS.
DR   InterPro; IPR014031; Ketoacyl_synth_C.
DR   InterPro; IPR014030; Ketoacyl_synth_N.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR   InterPro; IPR013968; PKS_KR.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   InterPro; IPR016039; Thiolase-like.
DR   Pfam; PF00501; AMP-binding; 2.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   Pfam; PF00668; Condensation; 5.
DR   Pfam; PF16197; KAsynt_C_assoc; 1.
DR   Pfam; PF00109; ketoacyl-synt; 1.
DR   Pfam; PF02801; Ketoacyl-synt_C; 1.
DR   Pfam; PF08659; KR; 1.
DR   Pfam; PF00550; PP-binding; 6.
DR   SMART; SM00825; PKS_KS; 1.
DR   SMART; SM00823; PKS_PP; 5.
DR   SUPFAM; SSF47336; SSF47336; 6.
DR   SUPFAM; SSF51735; SSF51735; 2.
DR   SUPFAM; SSF53901; SSF53901; 1.
DR   TIGRFAMs; TIGR01733; AA-adenyl-dom; 2.
DR   PROSITE; PS00455; AMP_BINDING; 2.
DR   PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR   PROSITE; PS50075; CARRIER; 6.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 4.
PE   4: Predicted;
KW   Complete proteome {ECO:0000313|Proteomes:UP000005258};
KW   Ligase {ECO:0000256|SAAS:SAAS01055201};
KW   Phosphopantetheine {ECO:0000256|PROSITE-ProRule:PRU00258};
KW   Plasmid {ECO:0000313|EMBL:AFK56006.2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005258}.
FT   DOMAIN      486    561       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     1495   1570       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     2545   2621       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     3873   3948       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     3961   4036       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   DOMAIN     4685   4760       Carrier. {ECO:0000259|PROSITE:PS50075}.
FT   MOD_RES     521    521       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    1530   1530       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    2581   2581       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    3908   3908       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    3996   3996       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
FT   MOD_RES    4720   4720       O-(pantetheine 4'-phosphoryl)serine.
FT                                {ECO:0000256|PROSITE-ProRule:PRU00258}.
SQ   SEQUENCE   5215 AA;  548170 MW;  C7C6626DAE06D298 CRC64;
     MSVIGRAGPG GRQDAAAETI AEAAPVSLLP LSGAQAGIWA DLTSGGVGPA GYNIADRIDL
     DGPLAPAALC TAIRLTDQAT EAVRLRFTTD ETGTPWQWLA EAPGRFDFAV VDLSDADDPD
     LLAQEAFEAL QARPFDTAQG PLCRHQLLRL APDRHRWIRC YHHLILDGQG GMILAGAVGR
     IYTALIRGEP VPDPDLPAFT DHLAREAAHA AGTGAGADAA WWRSRLAGLP AAARFRPAAT
     AAARFDGRIA SVTRPLGAAR LAAMDRVARA HGLASRSSLA LALFLVLLGR VAGTDTPVCN
     TPLGNRMGRA ERRTPGEFAY TVPTGTRIDG TESLAGLARR LDAMTRRDLR HMRLAPMRWP
     EAGFIPDRPA SRGALFNAMD LPATYGFDGL QVRVADSIFG ELHDVMACLR TYGAADASDM
     QWSFPPARID RARVESLAAA FEAMLDAALA DPTQPVDHLP AGDLPSPADT AGPVPPQPAV
     PLIDTTTRTA LEAEVTRLFA GLLDAPQLTA DDDLTAHGLQ SLRAIRAVAP LTRLIGREVT
     VADILGHPSA AALVGHLFGR LETMAAADES RHAEVDSPAE ITSFRLPARL EGQWLARRDA
     GPGTSYSIGL VCDLPAGTRA AALEEALHRL LDRHPALRST FAEGVDGEVM QRIGPVPPIR
     LAGYDDAAEM LAPFDPATGP LIRFGWEADQ PERLRVVVDH LVFDGESRTV FQRELTALLT
     GQPLPPAPPV DAGGLDEAAE AAARAFWAPR LARLPERALA AEPDPVPSGE GRRAMIRLAP
     ETTRALHGLT GRGATPTAVW TAIATTALWR ILEDTQRLSA GIAFAGRSRR DTTDAIGCFV
     NLLPVVVEPT AGEALPQLAR RIGRDISQVL TVQDYPLSRL AADWAQAVPA RGPTPVDLVC
     TLENDLSALE AADISFATGK FPLLIGLMWQ GDAAALNIEY DPSRFGSAWI GRLQTVITRL
     IAAAAADPAT PIDRLDLLDA GDRAVIAAAN ATEFTWPRDG GLGRQLDQVL RADPDRIVLA
     DDARALTARD LRARLGGVAA ALAAVGVEPG TPVALAVERD PDGLTALMGI IWAGNAFLPL
     AATFPVATAR ELLATAGCTV LVADDAGLER WAALAPGITL IPLPTGRADL PPAPRGPEDP
     ACLLYTSGST GMPKGVVVPH RAILRLAYGD FCTPGGCMAQ AAPMAFDAIS LELWAPLLNH
     GRVRILPATT MFDPPLLERR IRDDGIDTMW VTASLFNQIV DERPGTFAGL KRLMTGGEVI
     SPDHVRRVMA ACPGVVLYNG YGPTENGTLT TIHRITAADL EGGPVTIGRP VPGTRVHIVD
     GRGQPLPIGV WGELVTAGDG LATGYAGRPE LTAAAFVTLD GLDEPLVYRT GDRARWRADG
     RIDFGGRRDG QLKIRGQRIE TAAVEAILAA APGVRDAVVA GIGHGADQIL AALVAADEAD
     EAGWRAAIAA RLPAYMVPER FRLVDRLPVN ANGKADRRQA AAMLAEAAPV ARPAGTGTGF
     ERAVITAFES LFPGVRIGIE TDFFDLGGHS LAAMRLSALL QASTGRRPGL AQIMAARSVR
     GIAALLDRLP AVAAQPAIPR ATGNEHPLSP GQLRLWVLHR LHPGLATYSV PMLLALDGPL
     DADALDRALV GLETRHHALR TRFVARPNDG DGVRQIVAAP GALTARRLCL DATGADQAIA
     REIARPFDLG AEPLARAVLI RIDAHHHRLL LVLHHAIADG WSLPVLLRDL GRLYAGAVDG
     RPDDLPPPAR QLADIAVWQR ARAAGAEGRA LIDRWQSRLT PPPQPLRLPA DHPRPAVRSF
     AGDILSLTLP MAATAAVDRM AAAAGVTPFT VLTALLQALL HRLTGARDIA LGLLVAGRGA
     PVPDDLVGFL VNTVVLRQSV DPGARFADHL AITTDRLAEA SADQDAPFEA VLQTLALDRD
     PARNPLFDVL VTWQDDMPAM PAMAGLTVRP LDCVLPFAKF DLSFHFQRDG GRIRLHLEYA
     TELFETATVR RLADRLAVLA AAPGLEGSAI GALPLMTDID RAALARWNDT ARALDTTRPL
     PTLRHAGDPS APALIAPGLT LDNQGFDRRV GGLARRLLAA GVRRGDVVAL VLPRSPDLLI
     AVHAVLAAGA AWCPLGPDLP PQRRAHMIED LGDPWFLSDA AHGTDLPADR LILIGDDLAD
     APVVPAGPDA LAYVLFTSGS TGRPKGVEIT RGALANRILW MQQTFPIGPA SIDAGDVILQ
     KTPAGFDVSV WELVWWAWSG AAVAVPPPGI ERDPQALAAA IRDHRVTVIH FVPAMLRAFL
     DALDDGRIEG AHLATLRLVF ASGEALDAAT AARFDRLLHA RFGTALHNLY GPTEATVDVT
     WQPCTPWDPA AKTVPIGRPV ANTRVLILDR AGRALPPGVA GEIVLAGPQV ARGYRGRPDL
     TADRFRPDPE IAGARVYHTG DLGRWTADGV VEYLGRIDDQ VKIGGVRLEP AEVEAALDAC
     PGVVRGLVRV GRRDGLAELE AWVMGAADLT PAGLRAALAD RLPAAMIPTR WFRIDQVPLS
     PNGKVDRKAL SGVPLAGRPA VAAAPAGHPA EAEIARIWRE VLPPEAVFGP EDGFFEAGGS
     SLMLLRLHDR LEARWPGRFS LAGLFAASTV AAQAALLDDG DTATGATATA ATDGAIAIIG
     IGLRVAGAED LAGFWQDVAA GADRVGPPAA ARLGSARHIA AAAGIAMPAT PRDAAWLDGI
     DGFDARRFRF SPADAALLDP EQRLFLETAQ CALEDAGLGG AALKGARVAV HVGGNANPLH
     RQALRHLSGA RLEQAFALNV PSNIATRLGF LNDWHGPASL VDTACSSGLV AVDAACRDLV
     RGTADVAIAG AARLILVPQG DDQRMTIESS TGRTHAFADG ADGTGAGEGA VALVLKPLAR
     ALADGDPVHG VILATAVNQD GASSGAAAPN PIAQADVIRT AWDRAGVPFA SVSYIEAHGT
     GTRLGDPIEI EGLTRAAGTV AFAEPAMIGS GKGNYGHLDN VAGALGLVRA VAALAHDTAP
     PQPFFDRPNP AIDFARAPVR VAARATRLPD RGTPRRAGVS SFGLSGINAH AVIEAAPPRP
     AAAMDPDGHA VIVLSAGDRA ALGRYARALH DRITADAGLD LAGIACTLTQ GRSHLAWRFA
     VAVPDRAALL AALDRLARGD DQDTAEVATG RQVRHQTVAA WHADPTAAAA AAAARAVLDG
     AVPVWPDFLP ARRHHLPPAP FSRIPCRIDL PAKAANSDID TTFSMGWTAF PGGRALALPL
     ADPAFWPVAE HRLAGRPTLV GMAIPALVAA DRGPGTVLHD LVWRRPLHAG SREASLVIAR
     DGSVTAGHAT AEGGWAVAAE ARVETSSAPH PSAVDLSTDG LIALDLAPFD GAEGVMQVGP
     RWDCRQAIWL APDRRRAVAR LALPAAAAAD RRLWPWHPAL LDIAASLLAG VGEVPREVAA
     IRILAPLPDT VMARADRRPD GLVDIRLADA AGRPCLLIDG LRFVAADGRA APELLAPVWV
     PAPLHPAPLP AGGCLVRNAD DLAAAPADAA LILQLPTGPD LVRRTADLLK IALARPGTRR
     IAVTGAGTET DPDRAAVAGL VIAAAHETRH QNLRYLDVDD DAAPAAVAAE WAAAVPERFS
     LWRDGLRHVR RLDPIAPETA PVWPDHGVAV VTGGTGGFAA ALAEALSHGG RVALALLARR
     ATADDTLATA IARIEARGGR VQLYATDITD QPALAATLAQ VRADLGPVTA VVHMAGIADG
     AMLAVRDMAG FDAVMAPKIQ GARNLDALTR NDPVTAFVVF ASLTGLVGAA GHTAYAAANA
     WLDGFARRRR AAGLPALAID WCALADQGMA ARAGADRAPG APVIFPAQAV GAMTGALSTG
     AVQVVVLPAG LAATLNAPPA PARPEPAATA MPAEPADLTT RIARIWAEVL GHDTVGADDD
     FFDLGGDSLA GLDIAERLTA ETGQPVTLQL LFDHTTPRAL AALLAPEAAE PVSTTIPAPT
     QTSPTLAHQI AAIWAEVLGH DTVGITDDFF DLGGDSLSGM EIADLITARL GQTATLALLF
     DHTTPAALAA TLSPPAALAA TLDPTETTPD PAPADPRRAP AGLDAWPLAW EQRAVVETEA
     LAGMGTAFNL PHEIDLPGDV DIDRLRAAVA ALIRRHAALR TGIIGADHAQ GGEGWPGDDD
     WYGGDDWYGG DDWRMVAIDA AIVTPSLAIE PIAHPVGTAP AAPIRPFSTD GGAMARWSLH
     RDPAGRLVLA FDIHHSMSDA QGVEILLTEL AALYQGRDLS PVQVDLRDYG WWSAVRGDAA
     IQAARAWWDG IFPATAQGRD LPGRDLPRLD LPADRPRPPV HGHDAAATGF DLPPALSRDI
     RALAARRRTT PFTVLLTGWA ILLSRLTGRD DITVALPVNT RAAAGFDGVV GMHAALVPVR
     MQVPADGSGA ALLAHIRDRQ AGALSHHALS LGRMLAEFQA PAMPERSPLS EVSISYMNHA
     ATAAGTLDAG GFRLRGLTRA DGKNDLSVFI ADKGEGFGLV LEYDTGLFDA ARIEALGPML
     QRILAGLTAD PDQPVGRLPL LDDETRGLLA AREAAEGVSV LDDRGQPVPP GVWGRLHRLG
     DDGWQPTGDR ARWRLDLTAI ETGGRLDRLT LWQGHLLDPA LIEAQLSGLP GVAALRILPR
     PGRLVAAITA AGPRPDPAHL RRAAGAILPP VLVPGAWHLL DALPEDDAAL AAGVAATPSL
     EEAAEAATER LATVLAVVSD VLGRPATDPS ADFHALGGHS LLAMRVVNRL EAATGIRIPM
     RAFFADASMA GIARSLGEAA SEATRAAAED VIPPGAGPGA HPASHGQQRL YLLQQMQPES
     PAWLMLFVLP AGAVDARALR LALDRLSARH ATLRTGFATT DDGIVQLVAA EAPPPLVVDD
     LRHRTDGMDE ALRLARHEAA TPFDLAAAPL FRARLIRVGD HESLLLLVLH HIVGDGWSSR
     ILLAELATLY HAATEGRGDP LPPLPASYAD YARWQQGRDW QAALDGWRDR LAGAPDAIAL
     PTDRPPRPDA SGRGATVRRE LPAEVLAGLS RVARRHNTGL AAVGLALFAA VLYRLSRQDD
     MVIGMGVAGR DRAETEGLIG FFVNVLPIRL QLDDETGLGQ LIDLTRDRLA EALDRRELPF
     DRLVRALAPG RAGGRQPLAN VIFEYQRFDD VKAATTEAQL PPPPADGGRL GSAVAGLVDA
     STAKHDLILF FEDGGDRARF SAEYDTDLFD AATIEAWTGY LLRIATAVAA SPDEI
//
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