ID   I3TTU4_TISMK            Unreviewed;       470 AA.
AC   I3TTU4;
DT   05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT   05-SEP-2012, sequence version 1.
DT   27-MAR-2024, entry version 43.
DE   SubName: Full=Putative aerobic phenylacetate-CoA ligase {ECO:0000313|EMBL:AFK56182.1};
GN   Name=paaK {ECO:0000313|EMBL:AFK56182.1};
GN   OrderedLocusNames=TMO_b0174 {ECO:0000313|EMBL:AFK56182.1};
OS   Tistrella mobilis (strain KA081020-065).
OG   Plasmid pTM2 {ECO:0000313|EMBL:AFK56182.1,
OG   ECO:0000313|Proteomes:UP000005258}.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
OC   Geminicoccaceae; Tistrella.
OX   NCBI_TaxID=1110502 {ECO:0000313|EMBL:AFK56182.1, ECO:0000313|Proteomes:UP000005258};
RN   [1] {ECO:0000313|EMBL:AFK56182.1, ECO:0000313|Proteomes:UP000005258}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KA081020-065 {ECO:0000313|Proteomes:UP000005258};
RC   PLASMID=Plasmid pTM2 {ECO:0000313|Proteomes:UP000005258};
RX   PubMed=22458477; DOI=10.1021/ja301735a;
RA   Xu Y., Kersten R.D., Nam S.J., Lu L., Al-Suwailem A.M., Zheng H.,
RA   Fenical W., Dorrestein P.C., Moore B.S., Qian P.Y.;
RT   "Bacterial biosynthesis and maturation of the didemnin anti-cancer
RT   agents.";
RL   J. Am. Chem. Soc. 134:8625-8632(2012).
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DR   EMBL; CP003238; AFK56182.1; -; Genomic_DNA.
DR   RefSeq; WP_014752935.1; NC_017966.1.
DR   AlphaFoldDB; I3TTU4; -.
DR   KEGG; tmo:TMO_b0174; -.
DR   PATRIC; fig|1110502.3.peg.4431; -.
DR   HOGENOM; CLU_035301_1_1_5; -.
DR   Proteomes; UP000005258; Plasmid pTM2.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR028154; AMP-dep_Lig_C.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   PANTHER; PTHR43845; BLR5969 PROTEIN; 1.
DR   PANTHER; PTHR43845:SF1; BLR5969 PROTEIN; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF14535; AMP-binding_C_2; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
PE   4: Predicted;
KW   Ligase {ECO:0000313|EMBL:AFK56182.1};
KW   Plasmid {ECO:0000313|EMBL:AFK56182.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005258}.
FT   DOMAIN          117..321
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
FT   DOMAIN          373..469
FT                   /note="AMP-dependent ligase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF14535"
SQ   SEQUENCE   470 AA;  52028 MW;  08BDB6BC7D08C6A8 CRC64;
     MTGPDYFQAP DYFQAMDYPT IVQAYPVGDD FVALGQRLSR DELFARQDAQ FRKCLAFAWK
     VPFYQRLWGE KGIEPGDIRG LADIGKLPTY SKTELMASVA AHPPMGDFHG LDTYPAGQRP
     PVVFQTTSGT TGKPQPLIYG AKSREVQNRL LARAYLWQGM RREDVVHSVY GHGLINGGHY
     VREAVSHWVG AQFMSAGTGI ETRSVNQVMM MREFGATVLV GFGDYVKRLA DVARAEGMEP
     GRDIRIRMIT GHIGAESRES MSEAWGGAEV YDWYGVGDTG IIAGHGPDQD GMYVMEDAQY
     LELLDPDTGL PVEDGELGDM VCTCLFKDDV FPIIRFNTHD VTEVMTGASS TGVNFRRIRG
     FMGRSDNMVK LRGINIFPTG IGALLSEERE EMTGEYICRV ARIEGRDEMT VHVEVRTGAG
     DAAMKAAYEK ILRTRLGVEI GVAFAAPGEL APLTGIEVRQ KPIRLIDERK
//