ID I3TU93_TISMK Unreviewed; 6469 AA.
AC I3TU93;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE SubName: Full=Amino acid adenylation domain protein {ECO:0000313|EMBL:AFK56331.1};
GN OrderedLocusNames=TMO_b0323 {ECO:0000313|EMBL:AFK56331.1};
OS Tistrella mobilis (strain KA081020-065).
OG Plasmid pTM2 {ECO:0000313|EMBL:AFK56331.1,
OG ECO:0000313|Proteomes:UP000005258}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Geminicoccales;
OC Geminicoccaceae; Tistrella.
OX NCBI_TaxID=1110502 {ECO:0000313|EMBL:AFK56331.1, ECO:0000313|Proteomes:UP000005258};
RN [1] {ECO:0000313|EMBL:AFK56331.1, ECO:0000313|Proteomes:UP000005258}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KA081020-065 {ECO:0000313|Proteomes:UP000005258};
RC PLASMID=Plasmid pTM2 {ECO:0000313|Proteomes:UP000005258};
RX PubMed=22458477; DOI=10.1021/ja301735a;
RA Xu Y., Kersten R.D., Nam S.J., Lu L., Al-Suwailem A.M., Zheng H.,
RA Fenical W., Dorrestein P.C., Moore B.S., Qian P.Y.;
RT "Bacterial biosynthesis and maturation of the didemnin anti-cancer
RT agents.";
RL J. Am. Chem. Soc. 134:8625-8632(2012).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; CP003238; AFK56331.1; -; Genomic_DNA.
DR KEGG; tmo:TMO_b0323; -.
DR PATRIC; fig|1110502.3.peg.4582; -.
DR HOGENOM; CLU_000022_18_0_5; -.
DR Proteomes; UP000005258; Plasmid pTM2.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR CDD; cd05930; A_NRPS; 3.
DR CDD; cd17643; A_NRPS_Cytc1-like; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 7.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 6.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 7.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR014988; Uncharacterised_YqcI/YcgG.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 4.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 6.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 7.
DR Pfam; PF00550; PP-binding; 4.
DR Pfam; PF08892; YqcI_YcgG; 1.
DR SMART; SM00823; PKS_PP; 4.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 6.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 14.
DR PROSITE; PS00455; AMP_BINDING; 5.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Plasmid {ECO:0000313|EMBL:AFK56331.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000005258}.
FT DOMAIN 1624..1698
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3061..3136
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 4101..4175
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 5578..5652
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 6334..6469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 6347..6369
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 6469 AA; 682183 MW; D8D17339FBA0B63F CRC64;
MNSGRMLPED RRRKPTPWTA RLIRDDGLVA TPPPAWLPPF YDLFHAKVTD PAYPCFFGTL
AERKGEMFYA WVDRADDLSG VPAAMATFLR LAAERPEEKN NFALFFPPEP EPLSHSAYRD
RFWRVLQHLH DHDPAPTFET LETEPEDAAW EFGFGGEQMF AVGCAPSYKA RDSRNLGPGM
VILFQPRSVF IDTITKREIG AEARAVVRKR LLAWDGQPVH PDLGVYGDTD NREWKQYFLA
DGNDPEAGRC PFLVRRDRSF ADTLTALLVG RGRHQADLPA IAAPADGAGD HAATGRHLTY
GALDARARDL AARLADHARP GDRVMLVLPA GPDYVAGLFA CLYAGLIAVP AGAGQGADPA
RLQAIRADAA PRILLTNKGS ESIMANMAIK GDETVIYTDT ASDAAPRSFE PVVASPESPA
FLLYEPGPAG SGPGARRGVM HSHGSVMAAL LVARRLLGLA RGDVVVNVLP PDQAAGLIGG
VLAAIFAGAK LVMADPDRVQ ADPVLWLRAI ASQSGEISGG TISGGTDALW RRCLDRVQAA
DLAGLDLSRW RVAWCATGTL PVEVLTALRD RLAPAGLAPT AFHPCHGLAE AGLLIAGATP
GAGARIVDAP RSHARIGTAT AAAPRLIACG PAAPGHEIAI IDPATRHPLP AGSIGEIWAR
GPAVARGYWN RPDATAATFD ARIAGSDDRE NTPWLRTGSL GALIDGDLVV CDSGEAPGLA
AEASLPATVG QEGFWIDWQL DPADARWNVS ARLQLAGDVA PEAVRAAMAA MVARHDALRV
RFAEEGGRLR QLIAPEPVFE WRAGADADPD EAARRPFDLA RGPLLRADLV PDGPGRQVLQ
VVVHHAVTDG WSAGIMLRDL MLALAGRRLR PAPDYAAAIR SVALDPDHLA HEIAWWRDRL
AGETPELHLP RRAGATPGGP GRRVSRMVSA TLAARLAEAG RARGATRAMM LLAGFAALLH
RHGGTAATRI GIARAGRGAA ASRHVVGFFV NTLVLHTDIP AGLDAAGLIE RVRDGLREAE
AHGDLPFPRL VAALRPDRRT GRNPLVRVMF NHQPGRLDRR LADGPAALAG IELDPGTAEI
ELSLGVSETA DGLAIAFDHA AAAFAAEDVE RLADDYLAIL EALATDPAQK LGALPVSLPA
PQPAVAPAFR SAAARIAAQA AADPAARALI VEGQSWSRGR LARWSDRIAR DLLDAGLAPE
ERVGLDLPRG AAMVTGFLGI LKAGGAVVPL DPALPAARRA LILADAGVRR VLTDAAEPEG
ADVAPVLEPV DPGQIAYVIH TSGSTGMPKG VAIPQDALSA HLDDYTARYG LTDADRVLQF
STLGFDAAFE QLLPVLAAGG TAVLRGPEIW DHATLNARLA ADRVTLAYLP TGYWRRWLDD
LPASLPCLRL MVVGAEALGG DAVGLWRAGP LGHVALRNSY GPTEATITAT DHETGPTDIP
SPQVAIGRPW PSRLAVVLDA HGNPVPVGGV GELCLGGVAL ARGYLNRPGL TAERFVPAAG
GGRLYRTGDL VRPRADGTLD FLGRADSQIK LRGYRIEPGE VEAALRRQPG VRDAVVGLMA
GRLVAHVAGT ADPAALTTAL GADLPGWMVP QAILVLDALP LTPAGKIDRR ALPSPTLAEV
AGEEARTTNE TLLLTIARAL LGRSDLGVTD DLFAAGADSI LVLQLVARAR AAGLVLTPRA
VFDWPRIDRL AAMAETGAAP ALLPPLTGTD LPLTPIQSRF VARHAEPVSP WNQAVLLELA
DDMGGDDIGA PAIATALGRL VALHDALRLR LRRDADGVWR QWIAAEEGAE LLVAPADPAD
LHAMVDPVDG PVLRAALIAG SPRRLMITIH HLAVDGVSWR ILLDDLATLL GGGEPAPATT
PWSRWVEALP ARAAAVQDQA DIWRRRLAGA IPTFSPHRAG RRRRAELIVD AAQARDEVLL
AALSRALGRP ALVEIETHGR DLAGPDTPLD RTVGWFTATH PMVVTPQDDD RVLVAGLRAA
RIGLPPGGAA HALVDGLDDL PRPDIAVNNL GRFAEGAGPL RLVTDMIGDP SGGHAPEHAL
VLDALTIGDR LHLRFDADEA ALDLDRFAER FAAALDALGR AGLPEDAWPA TPMQQGMIFH
GRLAPADGLY VNQLCLDLAG RIDPAALEAS WNAALARHPI LRSRFAETGD GSLIQIVEPE
ARLRLNCIDV DADGYYIDVD ASGDRYIDVD AFLRADAARG IDTATAPLMR LTLLRGVRAD
TLVWTCHHAI TDGWSSARLL AEIAGLAEIA GAGQGGSYRD YVVWAAGRPV DAGWWRARAA
AVTDPVLLLP ALGRPEAPVP GRACRTTRLD AARTAALKSA AAAAGVTLAT LVQGAWALTL
ARLGGRNQVA FGVTVSGRPA DLPGVEAIQG PFLASLPVWV DLPGSAPLAP WLRVLQSETV
DLAGHAHTGL AEIGRITGLG AEGLFDSLLV VENYPLDPRL MARGFGAPVT DARMVERTHF
PLALAVIPEA GRSGEGECLA LRFDHDATRL DHLTIAALAE GLADLLGQLA ADINQDLGNI
ALALPGGDAG GQRDGAGDAA TPFTPVLTPI SAHAAADPDR PAVTMGDDRL TRGALDRLSN
RIARHLARAG VATDRLVGLC LDRSPALVAG ALGILKAGGA YVPLDPAYPA ERLAGILDDA
GLQVVVTDQA TADALADLFA GRTLVLVDAA DEADDSPLNT PIHPDQLAYV IYTSGSTGRP
KGVGVTHRNL ARLLTRTSHW YGFGPADVWP LFHSFAFDVS VWEIFGALST GGRLVVVPWW
TARDATAFHG LLRAERVTVL NQTPSAFLPL MQADMAASDR LTDLRVIVFA GEKLEPAGLA
DWLAWRGDSA PVLVNMYGIT ETTVHVTYRE TTAADATRQP PRSLIGVPIP DLSLHVVDAD
LNPTPVFGTG ELLVGGPGLA RGYLGRPALT AERFIPDPFG APGGRLYRSG DLVHLMADGM
LDYVGRNDLQ VKIRGFRIEL GEINAALLTA PGIREAAALA VDDGAGAKRV LAWLVMQPGV
TLDAAALRSH LAARLAPHMI PAGFIALDRM PLTVNGKLDR ARLPMTIAPE AGSDDGPGYE
KPATATEAAL ARIWAGVLGL ARLGRTEDPF LNGADSLLAM RTLARIRSEL GADPGLKALF
DHPTVAELAK IVDAAKEHDL EVLPARADTS RAPLAPGQDG LWFLWQLDPA STAYTVAGAL
SLRGAGAGTV DATTLRRAVA DVVARHEALR VRFAETPTGP VQLIDPAPRF VWQEIDLTAA
PETLPDLLAG FAAEPFDLTA GPLLRARLVR LAPDHCVLAL ATHHIVADGW SMTVLAREIA
ALCQGRPLPV PVSGWGDWSA SARAALDQGR LADQIAWWQA ELGRDHPVLR LPVDRPRKGA
RSDAGGRVER RLDAGAMGRI RAAGRGATLF VTLLAAFDIL LHRQGGDTDI RIGVPVAGRT
GPGTEDMVGF FVNTLVLKAE ITGGMRFGDL VDQLRRRSLE AWSRQDVPFA VLVDRLGAER
SLERTPLFQV MFNLQQQDPA AAAPGEGIEV EVVENPVATA RFDLVLDVVE RPDGLRLAFD
YAADIFDAAT ITGLADQYLY LLEQVTSGAD PLIAALALPA PRPAALPSAP IRQRFDPVAD
RILRRAARVP AREAVACEGI RVTYGDLDRL SNRIAAALLA AGLRPEERVG LLLTRTADLP
AALVGVLRAG GAFVPLDPAY PEDRLADMAA DAGIRLVLAD AATEATLPGL LAGRQVIRLD
ALPTGGQMAP VLVRVHPDQL AYVIYTSGST GRPKGVAISQ DAFSLHLDDF LATYGIGDGD
VVLQQSTLNF DVALHEMLPA LIMGGRIVMR GPAMWDLAAL SRVLAEERVT FSRIPTAYWQ
QWLRDPPRDL PHLRQVTVGG EGLPGDALRR WQEGPLGHIR LDNLYGPTET TVACMHRITT
PEDAGALIVP IGTAYPGRTV AIIDADGNHV PVGGYGELCI QGDSLARGYL GRPGQTAERF
VPDEQACRQG APGARLYRTG DLCRMRRDGT VDFLGRLDAQ VKLRGFRIEP GEIEAAMRAC
PGVEDAVVAL IGSGEGARLA GYVTGTAEPA VVAAALGQRL PAHMVPTTIT PLAALPLMPN
GKVDRKALPV PELAAAGEIV APGTDAEAAL LDVWRGVLGR EDFGVTDGFF ELGGDSILSL
QVVARARAAG WVITPTQIFT HPRIDRLAAV ATPAAAQDIA AEITGTPLPL TPIQRGFFDR
HPDGPSHWNQ AVLLIPDPSV TAEALAAALA ALVARHDALR LRFAVDGDGA WTQHVAPAET
VELLICRTTT RDGLEDACTD VQASLDIRTG PMLRAGLFEL PGGARRLLIA IHHLAVDGVS
WRILGADLET ALSGAPLAPV PMPWSRWVSD RAALAATPAL ADQAAWWRQA AEGAEPCFPD
RTGPRGLTQS WQADPALTAA LIHDLPRRYG LGVEDLLTTT LARLLATTTG RTHIGIDLEG
HGRDTDAADA GPAADLSRTI GWFTARYTLA LPGAGDTADI LAATRQTLKS VPHGGRDRGW
ITALDDLPRA DVSLNYLGRV DLGLAGNGRV ALAPEGTGRR VAPGTEGSGH ALAADALIRD
GRLVIDWRSD GALADGAALH SLAEHFGTLL AGLVETCRTT PPVLVPADVP LAGLGAAALA
RLGLSLGTTE DVYPATGLQE GLLLHAGEAN DGLYINQLYL VLDPETDIEA LVQAWRAAMR
RHAILRTRFA WDHGGAALQI VMRDAVLPVA RLDWRNLDAA GHDRRLAAWA AEDRRRGIDP
AVAPLARLTL IARPDGGHDL FWTSHHALSD GWSLALLMGE IAADYAARID GTEAGLPPVR
PWRDHVAATL DAGRRFASWW QAEAARRDDP ARLLPLLTAP TVPAGEAAAG AFTRHTPLGE
AATARLAAAA AQAGVTLATL VEAAWALTLG RLGHRNQAVF GVTMSGRGGD LAGIDRMIGL
FINSLPLVVD LPGAMPVNQW LQNIQTAAAS LRAAEATPLA TVQRLTGLAG DALFDSLLVF
ENYPVDGALA DSRLGRLVRH VAMVERTHYA ATLAAVPGST LDLRWGFDGA RVSPSDADRL
AARFLDLLTA LPDHLDRPLG SLAWPADPVP QAAAQPFRAV TARLAAAAAA HPNAIAVTCE
GVSITHAALD RHANQVAGRV ADAGGTPDLT DTPIGLCVSR GPALVAGLVG IVKAGAVAVP
LDGDLPDARL AQIIAGAGLS LVVADAAGRD RLAGFGLTII SADEGGDAAP AVDAVRHPQA
LAYLLYTSGS TGTPKGVAIP HGALAAHLDD HAARFGLTAD DRVLQFSTLA FDAAFEQLLP
ILSLGGTVVM RGPDLWDYAT LNHRLAADRV TVAYLPTGYW RGWLAALPEV APASLRMLAV
GAEALGGAAL GRWRGGPLGH LPLLNTYGPT EATITATCHL TGAADIAAAQ VPIGRAWPSR
RVLILDTHGN PVPEGGIGEL CIGGPALARG YMARPGQTAE RFVPDATGGP GARLYRTGDL
CRREAGDVFR YLGRIDDQVK LRGYRIEPAE IEARIRAVPG IRDAAVGVIG QGEAARLVAH
IVGEAGPETV RAALARDLPG WMVPQAILPL DALPLTPAGK LDRRALPAPA ADLPTHRAPE
SPAERMMAGL WSAVLGIENP GVTDDWFALG GDSIQSLQLV ARARAAGLKL TTRQILEHPR
IADLARLAVP ISEANDLPAA PATDPFAAVR QVFAELGEPF DAVEDVYPAT PLQQGLLFHA
RTGARDGLYV NQLRITIDGP VDRTALADAW QAAVARHPIL RTRFEWRHGG EALQVVQRSA
TALVTEEDHG LTHIAAHDAR LRAWRAEDLA RGFDLTRAPL ARLAILARPD GGNDLIWTAH
HVITDGWSTA RLLAEIATDY AARQAGGAAD IPAPRPWRDY VAWAAARPDA AAWWQAQAAR
LADPVTLATA FGDVTPAEPD TTDDISISLG DNLSDALRRL ARRNGVTLAS VMQGAWALLL
ARAGGGRQAL FGITTAGRPP ELDGVEAMQG PFINSLPLLV DIAPGRTLGA WLRDLHALGG
ALREVEHTPL ARVQRWMGRS GDALFDSLLV FENYPADRAL GDTAFGGRIA GVETTERTHY
PLTLAIAPDA AITLGFGFDP ARLPADLVAR IAGLYRDLLA RMAEAPDDLA LGALRLTDQG
MPVVNAPATP DVVTRIAARI AATPTAIALR AGGEGRDYAA LGLQAGGIAR RLTGEAAVLP
PDTSIGLCVA RGPDLAAGFL GILAAGGAVL ALDPDLPDAR LRLMLEDAGV TRVVVDAMGR
ARLAGFARDL TLIDVAGAGA PLAAPHLHPG QIAYLIYTSG STGRPKPVAV SHGALAVHVD
DYIVEYGLTA DDRVLQVSTF GFDAAIEQLL PALTSRGKGG DARPRPLVGG GDHRRTGRRG
GDRRLSAHRL LADVAGGAGS GASPGTPPGH HRRRGAGRGG AGRLAGEPPG PCALPEQLWP
DRGDHHGVGP PQPGRRCPAA GGADRRGLGR AQPGGARYRG RPGSGRRNR
//
