ID I3WGI8_BIFBI Unreviewed; 1414 AA.
AC I3WGI8;
DT 05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT 05-SEP-2012, sequence version 1.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:AFL04001.1};
GN ORFNames=BBB_0406 {ECO:0000313|EMBL:AFL04001.1};
OS Bifidobacterium bifidum BGN4.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=484020 {ECO:0000313|EMBL:AFL04001.1, ECO:0000313|Proteomes:UP000006173};
RN [1] {ECO:0000313|EMBL:AFL04001.1, ECO:0000313|Proteomes:UP000006173}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BGN4 {ECO:0000313|EMBL:AFL04001.1,
RC ECO:0000313|Proteomes:UP000006173};
RX PubMed=22887663; DOI=10.1128/JB.00988-12;
RA Yu D.S., Jeong H., Lee D.H., Kwon S.K., Song J.Y., Kim B.K., Park M.S.,
RA Ji G.E., Oh T.K., Kim J.F.;
RT "Complete Genome Sequence of the Probiotic Bacterium Bifidobacterium
RT bifidum Strain BGN4.";
RL J. Bacteriol. 194:4757-4758(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
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DR EMBL; CP001361; AFL04001.1; -; Genomic_DNA.
DR KEGG; bbf:BBB_0406; -.
DR PATRIC; fig|484020.3.peg.402; -.
DR HOGENOM; CLU_003630_0_0_11; -.
DR Proteomes; UP000006173; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000006173}.
FT DOMAIN 21..420
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 421..808
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 216..236
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 42..49
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1414 AA; 155040 MW; F90B978DF0E160AF CRC64;
MRRRNQHRLR NEAVMNAPRF TDSPEQAKVV NAPTGDDVLV VAGAGSGKTY TMTRRIINLI
AQGVPAERIL GLTFTRKAAS ELLSRVSAAV LADGGDERFA NRAFLKPEVS TYDAFFQTIV
RQYGLLVGFD QNTQPLSQAG AIQLATTVVG RHMDILFEQD LGAFKTVVNG VLGLSHAIGN
AMIGGSTTTM DEAIGRVRAW DQAFLAQLDI AIGDTPVPDE APKAKAPTKN KKDTEETFAA
KQEEYRAQLR DICVYKCAQL RDVTRRRETL LTLVEEYERE KRVQNMAEFS DFTIAAYQLV
ARFPSIGERY RRRYTHVLLD EYQDTSTTQA MLLATLFHPQ SDADADRSSS RWRQAARSAG
GWSKDGVGLS RSAVNAVGDP FQSIYAWRGA SPGAFRMFQK DFGMPADAKP YPLSVTRRNT
CIVLRAANNL TEPLRIPPRR AGSSLMREVD VANLSAMDNA AEGTIGVLGF ATFGQEADAV
ARFAKQAIAR YRKDDAATAA SDTPHVAVLF RGKTHMAQFA QELERAGLTT LVVGYSALLE
RPEVQDLIAL LHAAADHTDS ASLMRLLATP RFGLGSESLT RLAGMAEDLD SQCRYRALVE
SGIIGSGSDG ARADDGHDEA IVSTSIDSGL VSEADGGSDI ATFADPQVKA IVKQYRDRVP
HAVFLIDMMM RDDLPSLLSR RGGFDETTSR RIIQAGEVLQ RVHAVTNHPL KEVIDAAVRA
LNLDIDTIVA QAVKNPSRPV NPTQARSPLQ SVAALVDTYT QEIVEGVNPT LRGFMSWVDS
LGQIEEDTAA VPDTPADVVL MTIHQAKGLE WDAVAVVSMQ SGVFPSNQGG LHVELDAEHP
GGGAGESWIP PEYKATVRSW ADDPAAVPAP VRVDAGILPR FPHDANPDDA PVETLRALDD
VEVIDDEFYG DLRSKDIGDD MDVADPSSWY LTQEEEYGRR LLADERRLAY VALTRAKKDA
LLTYSQYSIL DRDPSQYLNR PGKRPSASKP SVFWTEMQDS LCHESGLVSA CKSGSAGYSE
NADAIADSSP DLAVAGAASG ASAHPSLDQL GVDRPQGFFV GDHATEYERD VVEEAWNAPL
EEREQAGDPT WPAKLSEETL SKLTGGLSGD SGLVTDDPDS LLARARMLVE DPDLMPWSFG
RGEREIDRYV RRQAEHLLEH GRQNVTALQA RAGHMTQREE RGYWRGLIRP IPRVASPAAE
AGTLFHAWAE QFINAYGDGQ SIDADGIGAY DSSSIETGTA GTCREPVTRE SLLHDLSELE
MKSDTLTAKE RQLTVWQRRL AESTWSERRP AWAERQIVVS IPQLGDVIVN GKLDAVFHGG
LDANDPTKQY TIVDWKTGRK PRKPQDIEHK LVQLDWYRLL LSYIEHVPLD RIDATLYYLS
EPDEGARELH ARAKTEQEIL AELSSGIPEG SDND
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