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Database: UniProt
Entry: I4N355_9PSED
LinkDB: I4N355_9PSED
Original site: I4N355_9PSED 
ID   I4N355_9PSED            Unreviewed;       590 AA.
AC   I4N355;
DT   05-SEP-2012, integrated into UniProtKB/TrEMBL.
DT   05-SEP-2012, sequence version 1.
DT   10-OCT-2018, entry version 35.
DE   RecName: Full=Malto-oligosyltrehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE            Short=MTHase {ECO:0000256|PIRNR:PIRNR006337};
DE            EC=3.2.1.141 {ECO:0000256|PIRNR:PIRNR006337};
DE   AltName: Full=4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
DE   AltName: Full=Maltooligosyl trehalose trehalohydrolase {ECO:0000256|PIRNR:PIRNR006337};
GN   ORFNames=PMM47T1_14596 {ECO:0000313|EMBL:EIK95895.1};
OS   Pseudomonas sp. M47T1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1179778 {ECO:0000313|EMBL:EIK95895.1, ECO:0000313|Proteomes:UP000004339};
RN   [1] {ECO:0000313|EMBL:EIK95895.1, ECO:0000313|Proteomes:UP000004339}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M47T1 {ECO:0000313|EMBL:EIK95895.1,
RC   ECO:0000313|Proteomes:UP000004339};
RX   PubMed=22887683; DOI=10.1128/JB.01116-12;
RA   Proenca D.N., Espirito Santo C., Grass G., Morais P.V.;
RT   "Draft Genome Sequence of Pseudomonas sp. Strain M47T1, Carried by
RT   Bursaphelenchus xylophilus Isolated from Pinus pinaster.";
RL   J. Bacteriol. 194:4789-4790(2012).
CC   -!- CATALYTIC ACTIVITY: Hydrolysis of (1->4)-alpha-D-glucosidic
CC       linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to
CC       yield trehalose and (1->4)-alpha-D-glucan.
CC       {ECO:0000256|PIRNR:PIRNR006337}.
CC   -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC       {ECO:0000256|PIRNR:PIRNR006337}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRSR:PIRSR006337-1}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|PIRNR:PIRNR006337, ECO:0000256|SAAS:SAAS00964676}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:EIK95895.1}.
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DR   EMBL; AJWX01000012; EIK95895.1; -; Genomic_DNA.
DR   RefSeq; WP_008370097.1; NZ_AJWX01000012.1.
DR   EnsemblBacteria; EIK95895; EIK95895; PMM47T1_14596.
DR   PATRIC; fig|1179778.3.peg.2930; -.
DR   OrthoDB; POG091H0CAL; -.
DR   UniPathway; UPA00299; -.
DR   Proteomes; UP000004339; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0033942; F:4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR022567; DUF3459.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR012768; Trehalose_TreZ.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF11941; DUF3459; 1.
DR   PIRSF; PIRSF006337; Trehalose_TreZ; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   TIGRFAMs; TIGR02402; trehalose_TreZ; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000004339};
KW   Glycosidase {ECO:0000256|PIRNR:PIRNR006337};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR006337};
KW   Reference proteome {ECO:0000313|Proteomes:UP000004339}.
FT   DOMAIN      126    500       Aamy. {ECO:0000259|SMART:SM00642}.
FT   ACT_SITE    259    259       Nucleophile. {ECO:0000256|PIRSR:
FT                                PIRSR006337-1}.
FT   ACT_SITE    292    292       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR006337-1}.
FT   SITE        381    381       Transition state stabilizer.
FT                                {ECO:0000256|PIRSR:PIRSR006337-3}.
SQ   SEQUENCE   590 AA;  65953 MW;  9A654865F3E9CF27 CRC64;
     MPLPTPETWP HGAQLLDSGL TRFALWAPDA FHVSVELEDG QSLPMLAQDD GWFVIKSHCP
     AGTAYRYQID GEMLVPDPAS RAQQGDVHGP SLVTDPNAYQ WQFPQWQGRP WHEAVIYELH
     VGVLGGFAAV EKHLARLAEL GITAIELMPL AQFPGERNWG YDGVLPYAPQ SSYGTPEQLK
     HLIDCAHGHG LMVILDVVYN HFGPDGNYLG RYAKGFFRQD LKTPWGDAID FRRPQVRDFF
     IDNALMWLLE YRFDGLRFDA VHAIEDPDFL KQMAARIRQQ VDGPRHVWLT LENELNQAHL
     LEEGFDAQWN DDGHNALHVL LTGETEAYYN DFAERPTEKL ARCLSEGFIY QGERNRHGEL
     RGEPSGHLPP NAFVLFLQNH DQIGNRAFGE RLSALCSTDA LRAATTLLLL SPMVPLLFMG
     DEGAANEPFL FFTDFHGELA EAVRQGRRGE FAAFAAFADA EQRARIPDPN DAQTFTASRP
     HFPQADSPWA RLYATLLHLR HQHLTEHLTG SRSLGAQVLA DGALYARWQL NDGSELRIDL
     NLSAHAVESS VPPVQYCLFD SRLDATGDRP ANTLPPYSAL VSLTPAGDDA
//
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