ID I6YBI2_MYCWM Unreviewed; 364 AA.
AC I6YBI2;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=tRNA-uridine 2-sulfurtransferase {ECO:0000256|ARBA:ARBA00011949};
DE EC=2.8.1.13 {ECO:0000256|ARBA:ARBA00011949};
GN OrderedLocusNames=WEN_02865 {ECO:0000313|EMBL:AFN65356.1};
OS Mycoplasma wenyonii (strain Massachusetts) (Eperythrozoon wenyonii).
OC Bacteria; Mycoplasmatota; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=1197325 {ECO:0000313|EMBL:AFN65356.1, ECO:0000313|Proteomes:UP000009005};
RN [1] {ECO:0000313|EMBL:AFN65356.1, ECO:0000313|Proteomes:UP000009005}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Massachusetts {ECO:0000313|EMBL:AFN65356.1,
RC ECO:0000313|Proteomes:UP000009005};
RX PubMed=22965086; DOI=10.1128/JB.01240-12;
RA Dos Santos A.P., Guimaraes A.M., do Nascimento N.C., Sanmiguel P.J.,
RA Messick J.B.;
RT "Complete genome sequence of Mycoplasma wenyonii strain Massachusetts.";
RL J. Bacteriol. 194:5458-5459(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in
CC tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-
CC cysteinyl-[protein]; Xref=Rhea:RHEA:47032, Rhea:RHEA-COMP:10131,
CC Rhea:RHEA-COMP:11726, Rhea:RHEA-COMP:11727, Rhea:RHEA-COMP:11728,
CC ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:61963, ChEBI:CHEBI:65315, ChEBI:CHEBI:87170,
CC ChEBI:CHEBI:456215; EC=2.8.1.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001042};
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DR EMBL; CP003703; AFN65356.1; -; Genomic_DNA.
DR RefSeq; WP_014850065.1; NC_018149.1.
DR AlphaFoldDB; I6YBI2; -.
DR STRING; 1197325.WEN_02865; -.
DR KEGG; mwe:WEN_02865; -.
DR PATRIC; fig|1197325.3.peg.616; -.
DR HOGENOM; CLU_035188_1_0_14; -.
DR Proteomes; UP000009005; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR CDD; cd01998; tRNA_Me_trans; 1.
DR Gene3D; 2.30.30.280; Adenine nucleotide alpha hydrolases-like domains; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR046885; MnmA-like_C.
DR InterPro; IPR046884; MnmA-like_central.
DR InterPro; IPR023382; MnmA-like_central_sf.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR004506; tRNA-specific_2-thiouridylase.
DR NCBIfam; TIGR00420; trmU; 1.
DR PANTHER; PTHR11933:SF5; MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1; 1.
DR PANTHER; PTHR11933; TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE; 1.
DR Pfam; PF03054; tRNA_Me_trans; 1.
DR Pfam; PF20258; tRNA_Me_trans_C; 1.
DR Pfam; PF20259; tRNA_Me_trans_M; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000009005};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 212..269
FT /note="tRNA-specific 2-thiouridylase MnmA-like central"
FT /evidence="ECO:0000259|Pfam:PF20259"
FT DOMAIN 285..359
FT /note="tRNA-specific 2-thiouridylase MnmA-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20258"
SQ SEQUENCE 364 AA; 41357 MW; 5888853C7FD02F71 CRC64;
MEEKKIVIAL SGGVDSAVSA YLLKQQGYKV IGVHMQNWDK QLNESELEGS CQGEKDSIMA
QKVASFLKIP LYIYNFTQEY WENVFKPYLS DFEKNLIGNP DLGCNSKIKF GVLLETVKKE
FGKGIKLATG HYARVTEEEG IYYLESCNNP KKDQTYFLSR LTQEQLREIV FPLSEFESKS
EVRRLAKEIG LPNWDKKSSA GICFIGKREF SSFLSSYVKG EGGEIIDVER AESLGKHKGL
CYYSINQRKG TCVSGQREKY YVCGKENSNL LVCRESVRDK YLIKEGCLVT NLHWIYKAPK
VGEQVLVKFK HTSNFISGKI EEIKSELMRL SHQASCASTP GQYVVFYSTN KKRCLGSGVY
HNSI
//
