ID I7J585_9CLOT Unreviewed; 322 AA.
AC I7J585;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=tRNA and rRNA cytosine-C5-methylases {ECO:0000313|EMBL:CCJ33556.1};
GN ORFNames=CAAU_1472 {ECO:0000313|EMBL:CCJ33556.1};
OS Caloramator australicus RC3.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Caloramator.
OX NCBI_TaxID=857293 {ECO:0000313|EMBL:CCJ33556.1, ECO:0000313|Proteomes:UP000007652};
RN [1] {ECO:0000313|EMBL:CCJ33556.1, ECO:0000313|Proteomes:UP000007652}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RC3 {ECO:0000313|EMBL:CCJ33556.1,
RC ECO:0000313|Proteomes:UP000007652};
RX PubMed=21421756; DOI=10.1128/JB.00193-11;
RA Ogg C.D., Patel B.K.C.;
RT "Draft genome sequence of Caloramator australicus strain RC3T, a
RT thermoanaerobe from the Great Artesian Basin of Australia.";
RL J. Bacteriol. 193:2664-2665(2011).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCJ33556.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CAKP01000082; CCJ33556.1; -; Genomic_DNA.
DR AlphaFoldDB; I7J585; -.
DR STRING; 857293.CAAU_1472; -.
DR eggNOG; COG0144; Bacteria.
DR Proteomes; UP000007652; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR031341; Methyltr_RsmF_N.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR011023; Nop2p.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00446; nop2p; 1.
DR PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF17125; Methyltr_RsmF_N; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000007652};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 1..300
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 231
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 133
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 178
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 322 AA; 37090 MW; AF0653600F56F4F4 CRC64;
MKLPDRFLDR MKFLLKDEFE AFLGSYKEDR YQGIRVNTLK IDYEEFFKIC PFEIKGEVPW
AKNGLYISEE KPGRHPYHAA GLYYIQEPSA MSVTPQLNIK PGERVLDLCA SPGGKSTQAA
CYLNGEGLLV SNEIKLKRAQ ILVQNIERMG IKNAIVTNNS PEELEKVFVG YFDKVIVDAP
CSGEGMFKKD KDALNQWSEE NVLGYQSMQR EILKSAAKMV KPGGLIIYST CTFSVEENEF
IVDEFLKKHE EFELINIEKN YGFREGFFEI LDNEELKKTA RLFPHRLKGE GHFLALFYKK
DGEVKSPQRI KIQYKKAEYI GL
//
