ID I7K5E1_9CLOT Unreviewed; 993 AA.
AC I7K5E1;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN ORFNames=CAAU_0690 {ECO:0000313|EMBL:CCJ32774.1};
OS Caloramator australicus RC3.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Caloramator.
OX NCBI_TaxID=857293 {ECO:0000313|EMBL:CCJ32774.1, ECO:0000313|Proteomes:UP000007652};
RN [1] {ECO:0000313|EMBL:CCJ32774.1, ECO:0000313|Proteomes:UP000007652}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RC3 {ECO:0000313|EMBL:CCJ32774.1,
RC ECO:0000313|Proteomes:UP000007652};
RX PubMed=21421756; DOI=10.1128/JB.00193-11;
RA Ogg C.D., Patel B.K.C.;
RT "Draft genome sequence of Caloramator australicus strain RC3T, a
RT thermoanaerobe from the Great Artesian Basin of Australia.";
RL J. Bacteriol. 193:2664-2665(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCJ32774.1}.
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DR EMBL; CAKP01000031; CCJ32774.1; -; Genomic_DNA.
DR RefSeq; WP_008908050.1; NZ_CAKP01000031.1.
DR AlphaFoldDB; I7K5E1; -.
DR STRING; 857293.CAAU_0690; -.
DR eggNOG; COG0610; Bacteria.
DR OrthoDB; 9758243at2; -.
DR Proteomes; UP000007652; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR040980; SWI2_SNF2.
DR PANTHER; PTHR42927; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42927:SF1; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CCJ32774.1};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000007652};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 297..498
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 993 AA; 115106 MW; 0F44314CF9EB4035 CRC64;
MSIDYTEKGF EENIEDFLVN INGYRKRRLE GQALDSFKKY AIDVEILFEF LESTQAKELE
KLKSVYKSLY KERIIERINK ELNKRGLIDC LRHGIKDYGV HLKLAYNKPA NSLNYTLLNL
YSKNIFTVSR QVHYSEKNNN SIDMLIMLNG LPIIVLELKN PLTGQTVDDA KLQFMHDRDP
KELLFQFKKR TIVFFAVDTE EVYMTTKLDG KDTIFLPFNK GNNGGRGNPE VEGNYKTHYL
WEEVFQKDSL LDILFNFVFI KKEQIKDSTG EIVEERENLI FPRYHQLDVV RNLEYDTKIN
GTGKNYLIQH SAGSGKTNSI SWLAHRLAKL HDDENKPIFH SVIVITDRRV LDKQLQDAIY
QIEHKAGMVA KIEKNSNQLA EAIKNGTRII VTTLQKFPFI IDKIKDVEER NYAIIIDEAH
SSQTGRSAAA LRVVLSAKSL EEAEELDSQE EDDMEDRINK EIAKLGPHNN ISYYAFTATP
KAKTLEFFGT QGIDGKYYPY HVYSMRQAIE EGFILDVLKN YVTYKTFYKI AKKIEDDPDV
PARKAARAIA KYVSLHPHNI AQKTEIIIEH FRQVTRHKIG GRAKAMVVTS SRLHAVRYKQ
AFDKYINEKG YKDLKALVAF SGTVIDDGIE YTEEGINGIR EKELPEKFKA DEYKILLVAE
KYQTGFDEPL LHTMYVDKKL EGIKAVQTLS RLNRTCKGKN DTFILDFVND PEDIRKAFQT
FYEVTELECA TDPNILYDLQ NELNAMQVYT DEEILEVVKL ELDKKIKSSK GQEKLNSILD
KAVSRFKSLT KEQQEQFKSA AAKYIRTYSF ILQIGPFADS ELHKLYVYLN FLLRKLPKAD
SIKLDLADEI ALEYYRNEKT FDGSISLVKE GEAVLQPTSF GKTIREEEKV KLSSIIEKLN
QRFGTDFTET DRLTIDQLEQ DSMNNEDIIL SVNSNDFDNF KLDYTKTFMD ILINRREENE
KLFLKIIMEE DFRNVIIDYL AKKVYDQLRA KSV
//
