ID I7LDB2_9LACO Unreviewed; 532 AA.
AC I7LDB2;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=BN53_01280 {ECO:0000313|EMBL:CCI84743.1};
OS Lactobacillus pasteurii DSM 23907 = CRBIP 24.76.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=1423790 {ECO:0000313|EMBL:CCI84743.1, ECO:0000313|Proteomes:UP000009311};
RN [1] {ECO:0000313|EMBL:CCI84743.1, ECO:0000313|Proteomes:UP000009311}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CRBIP 24.76T {ECO:0000313|Proteomes:UP000009311};
RA Cousin S., Bouchier C., Loux V., Ma L., Creno S., Bizet C., Clermont D.;
RT "Draft Genome Sequence of Lactobacillus pasteurii CRBIP 24.76T.";
RL Submitted (JUN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCI84743.1}.
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DR EMBL; CAKD01000010; CCI84743.1; -; Genomic_DNA.
DR RefSeq; WP_009559298.1; NZ_CAKD01000010.1.
DR AlphaFoldDB; I7LDB2; -.
DR STRING; 1423790.BN53_01280; -.
DR REBASE; 69848; M.Lpa2476ORF1280P.
DR PATRIC; fig|1423790.3.peg.698; -.
DR eggNOG; COG0286; Bacteria.
DR OrthoDB; 9814572at2; -.
DR Proteomes; UP000009311; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000009311};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..166
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 178..494
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 499..526
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 532 AA; 60275 MW; D13E6C313F96E223 CRC64;
MDNSIEKIGA KLWEMANELR GNMDASEYKN YILAFMFYRY LSEHQEKYFA ENVGLTAEDG
KTLNDDYVEK AAEYGEDQFI KLASDNLGYA IAPNDTWASL IKRIDEGEVI PSDYQAMFDN
FNNNAKLNVQ ANKDFQGVFN DVNLGDNKLG TSTTLRAKSL SKIVKLVDSM EYTDENGKDI
LGAIYEYLIK QFASSAGKKG GEFYTPHEVS IVLSKLVTLG EVDQDKEFSV YDPTCGSGSL
LLTVGNQLPA GMKDKIQYFG QELNTTTYNL ARMNLMMHNV DYFSMHLSNA DTLEQDWPDG
PDAQGIDRPR FFDAVVANPP YSAHWNNAES KLKDPRFSDY GKLAPKTKAD YAFVLHSLYH
LNANGKMAIV LPHGVLFRGA AEEMIRKNII DKNYLDAVIG LPANLFYGTS IPTCVVVFKK
NRSNKDILFI DASKDFEKAK NQNRLTDENI DKIIEAYKER KDIDKYAHVA SLDEIKENDY
NLNIPRYVDT FEEEPEIDLA EVNRQLKEDD AEIAELEKKI NEQLKLLGVD EI
//
