ID I7LX18_TETTS Unreviewed; 380 AA.
AC I7LX18;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU000441};
GN ORFNames=TTHERM_00537060 {ECO:0000313|EMBL:EAS03271.1};
OS Tetrahymena thermophila (strain SB210).
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=312017 {ECO:0000313|EMBL:EAS03271.1, ECO:0000313|Proteomes:UP000009168};
RN [1] {ECO:0000313|Proteomes:UP000009168}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB210 {ECO:0000313|Proteomes:UP000009168};
RX PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA Hamilton E.P., Orias E.;
RT "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT model eukaryote.";
RL PLoS Biol. 4:1620-1642(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC {ECO:0000256|ARBA:ARBA00005163}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU000441}.
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DR EMBL; GG662495; EAS03271.1; -; Genomic_DNA.
DR RefSeq; XP_001023516.1; XM_001023516.3.
DR AlphaFoldDB; I7LX18; -.
DR STRING; 312017.I7LX18; -.
DR EnsemblProtists; EAS03271; EAS03271; TTHERM_00537060.
DR GeneID; 7826613; -.
DR KEGG; tet:TTHERM_00537060; -.
DR eggNOG; KOG2617; Eukaryota.
DR HOGENOM; CLU_025068_2_1_1; -.
DR InParanoid; I7LX18; -.
DR OMA; ESENCFG; -.
DR OrthoDB; 642531at2759; -.
DR UniPathway; UPA00223; -.
DR Proteomes; UP000009168; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06108; Ec2MCS_like; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR011278; 2-MeCitrate/Citrate_synth_II.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR NCBIfam; TIGR01800; cit_synth_II; 1.
DR PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR11739:SF25; CITRATE SYNTHASE-RELATED PROTEIN DDB_G0287281; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000009168};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369}.
FT ACT_SITE 262
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 316
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 380 AA; 42478 MW; 2B53CE585938511E CRC64;
MAEKTKKSGG GLAGIVAGDS AISTVGIGMG LNYRGYDIVD LAKNSTFEDV AYLILFGKLP
TSKQLEEFLD QIVKARYLPA QLKEILERIP VDVHPMDMMR TVSSFLGLIE PETKENDQYK
VAIRLLGIFG PAMAYWYHFA HSGVRINEQT GPKDTIACNL VKLLHLSQTA DPLIVRAVDV
SLILYAEHDF NASTFAARVT VSTLADFYSG ITTAIGTLRG PLHGGANEAV MQYLENIRSI
EEANSFLTES FKSKKLIMGF GHRVYKNGDP RSDIIKEYSK LLAEKQPKFG KKILYQISEH
IETRVVNEKK KFPNLDFYSA STYHQCGIPT ELFTPIFVMS RVTGWAAHIF EQRANNKLIR
PTSNYTGPQK LSFVPLNQRL
//
