ID I7MGV0_TETTS Unreviewed; 2623 AA.
AC I7MGV0;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 2.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Poly [ADP-ribose] polymerase {ECO:0000256|RuleBase:RU362114};
DE Short=PARP {ECO:0000256|RuleBase:RU362114};
DE EC=2.4.2.- {ECO:0000256|RuleBase:RU362114};
GN ORFNames=TTHERM_00502600 {ECO:0000313|EMBL:EAS02069.2};
OS Tetrahymena thermophila (strain SB210).
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=312017 {ECO:0000313|EMBL:EAS02069.2, ECO:0000313|Proteomes:UP000009168};
RN [1] {ECO:0000313|Proteomes:UP000009168}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB210 {ECO:0000313|Proteomes:UP000009168};
RX PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA Hamilton E.P., Orias E.;
RT "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT model eukaryote.";
RL PLoS Biol. 4:1620-1642(2006).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; GG662548; EAS02069.2; -; Genomic_DNA.
DR RefSeq; XP_001022314.2; XM_001022314.2.
DR STRING; 312017.I7MGV0; -.
DR GeneID; 7827201; -.
DR KEGG; tet:TTHERM_00502600; -.
DR eggNOG; KOG0504; Eukaryota.
DR eggNOG; KOG1037; Eukaryota.
DR InParanoid; I7MGV0; -.
DR OrthoDB; 239153at2759; -.
DR Proteomes; UP000009168; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd07997; WGR_PARP; 1.
DR Gene3D; 3.90.228.10; -; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 7.
DR Gene3D; 1.20.142.10; Poly(ADP-ribose) polymerase, regulatory domain; 1.
DR Gene3D; 2.20.140.10; WGR domain; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR012317; Poly(ADP-ribose)pol_cat_dom.
DR InterPro; IPR004102; Poly(ADP-ribose)pol_reg_dom.
DR InterPro; IPR036616; Poly(ADP-ribose)pol_reg_dom_sf.
DR InterPro; IPR036930; WGR_dom_sf.
DR InterPro; IPR008893; WGR_domain.
DR PANTHER; PTHR24178:SF9; ANK_REP_REGION DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR24178; MOLTING PROTEIN MLT-4; 1.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 5.
DR Pfam; PF13637; Ank_4; 1.
DR Pfam; PF00644; PARP; 1.
DR Pfam; PF02877; PARP_reg; 1.
DR Pfam; PF05406; WGR; 1.
DR SMART; SM00248; ANK; 24.
DR SMART; SM00773; WGR; 1.
DR SUPFAM; SSF56399; ADP-ribosylation; 1.
DR SUPFAM; SSF48403; Ankyrin repeat; 4.
DR SUPFAM; SSF47587; Domain of poly(ADP-ribose) polymerase; 1.
DR SUPFAM; SSF142921; WGR domain-like; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 7.
DR PROSITE; PS50088; ANK_REPEAT; 7.
DR PROSITE; PS51060; PARP_ALPHA_HD; 1.
DR PROSITE; PS51059; PARP_CATALYTIC; 1.
DR PROSITE; PS51977; WGR; 1.
PE 4: Predicted;
KW ANK repeat {ECO:0000256|PROSITE-ProRule:PRU00023};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000256|RuleBase:RU362114};
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU362114};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000009168};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU362114}.
FT REPEAT 695..719
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 729..761
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 795..827
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 1332..1367
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 1368..1406
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 1452..1484
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 1485..1513
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT DOMAIN 2012..2110
FT /note="WGR"
FT /evidence="ECO:0000259|PROSITE:PS51977"
FT DOMAIN 2138..2273
FT /note="PARP alpha-helical"
FT /evidence="ECO:0000259|PROSITE:PS51060"
FT DOMAIN 2283..2501
FT /note="PARP catalytic"
FT /evidence="ECO:0000259|PROSITE:PS51059"
FT REGION 105..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1075..1097
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1174..1207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2491..2570
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..149
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..229
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..265
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1180..1197
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2530..2570
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2623 AA; 299110 MW; 5E749AEE50DCF703 CRC64;
MADKGIFIYA VRDEDSMQGF ILVASEVDID VENSKAEKVG VYHIHSVKNK PYNYTVNKKL
TNVRLDEFMS VVDEDLYEFN EKNRTLVFDK KGYDNLIGQL MDDLEEENDD DSSEEEEKKK
KKPAPKAASA RKTRSSKGSD KVKGSDSESY EAADEEDEEE KKRSRGRPKK NKSTEEITED
KDENSQEDQP KPKGKRGRPP SKDKAASVAV PKKQDKQDDK DGEENGEDQN GHEENGDEDK
EEGEQKRVPG KRGRKPGKKT AETPAKKEAQ QNIIHNRKPQ VFLIQRPGKR GRKPAKKGSE
SEDNEDADDE QEEVSKKGAK KKVEKYKKGS HNPNIPDVVK TDETFEGDSQ DINDVCCSFC
TNRELLRAVY TGNSNLLKKL VQSKAKVSTL FAKRGPDADE TTLEIILKNK DKKCLDVLIN
ALNKKSEISL ASNPECSLEE VYTGFNDRFA YGVPTRKVQM ARGGKEGNNA FVADINSFVD
TQSWNENERL IFSLLENETE VQFLEFLRGL KDENLTTSLL EKTYVAVRAG NRKTASYLMK
QANKFGGYGF NNLHEQVLSL DDVSKLDEFK KVSITKKSTG VYSKNSYGFG GAQGIITPIH
CAAINPNPEF IKKLLEISPE FTIPDDLMRK PVHYAATCEG PGPLSYLLKQ GVDAREGDKK
KITPLMLACQ FGRAKNVEIL LDANGKSNPD TRTREGYRAI HYAAHYGHLD CIKLMIEKGG
VEVDSPGPKR MTPLHIATSR GHFEIVEYLL SKGAKVIVKD KFKRSAVIHA TANGYLKILS
LILKNGGPFN DPDSSGNYPV HYAAAYGFQA CLDLLKQAGA DFNPQNSWNL TPLSIAMAKG
HFGMVKKLLN YPECDVNCTD VKGRTIISRA TEKLNLNNLS QFEFILKEKK ANPNMADLQG
QSPLHYLCNL SKDDYVSKRQ NEENAHHVTK KYEEEYEKIV QKATQILLDC GADINKLNTM
EESPLTIAIK QQNFFILNIL LKKGGLDFSH VNQIDQNILH TLNTLILEGP SGIDQFYNIF
EEIKKDKNVN LKEYLNKVDY HGFSPLLMYV KIFSENASHY REQRVEELSQ EIYSKKGGSA
PNKKNPIKKQ APTRGPRVAK KAIRSAPAKS LIGFSNDGAL VQLTEKELEK VQKQADEDLL
QFEQKFQKLI TFLVEKGSDV EAKVQKIKKY RSNMDVEDKS DNEEQQEQDE DDDENEGNKT
LKDEMGNPIQ NKKVKYIPEY NSCGEMNIFG LALSHLSLPY LKFIHEQYPQ LVNNVDYLKR
NHLHKFILKN DDEPATNVSH KVLEWLTTLK IDINQKDIIE STPLISAISQ KKYKFAKTLI
NHKAQVDVKD IFGKTPLIMA INNIQSNNLE FVEYLLSNGA SANFADNYGR TPLHHAINKA
DKGANASFDL ENLLIKNKAD INAADSLGRT PLHYCFVSLQ DQQYSTLQYD PFETVSSLCN
QSNIKIDVKD NLGKTPLHYA AQKNSTISVI YMIEKKANID IEDANGNTPL AVAFRFKRSE
ITTTLIQNGA NTSKFITINK NLKPNGEEEE EYKEPAKDHW GNPIKTRRRQ NYSSYGSRYL
FQYQNQYDGF GGDQNSNNSI IKKLLSEYHI EEDSYIELVK RSITKEQFKE KGLDNLVFQE
FRVRGDLPLG KHSFFKASIT FQWQGIAYLL LIKGNYNLFN ALEDAIKIDL YQLSITLLFK
YPEDETITQC NSEGQNLIHI LCQRSPRDVN LQVKLFETLL NRGVDAKKLD NKKNLCLHFA
FLHHQLTPKL LELGLDCNAY NDDQQTPFAL AIQEDAKQAL KIYTANYKPD YSKKFKAKLE
GHQDYLQFTP LLIQFANKNK DLVLLNHLLA NGSNINEVDN LGRNALYYAI YHNSHKLVKF
VLQTKKFDVK HVDNQGKNAV HYVVNPVEFG SYENTEILEL LHNAKIDFNV KDKDGNTAMF
YALQQDSKKM AESLKKYGVE ALKEKKLPQR AATSIVTDAL FPDEEIDYEE DSKKFSEETT
YDQYKEFFEQ KNAGDKVYLD KYVAGNYKKK DNYVVLDDNN EPYNLLMTKV DLGGGFAKYV
FYIMQVIHDK NSDVYILYTR WGHMGGMGQM QHTPFATKEA AVKEFSKIFY QKSGNDWTNR
HNFKKQDKKY QLVIFEKGND KKSFSLKPYD FERLNCSASQ LEKPIQEFIK NISNAEIFKQ
AYRQFALSDQ YLPITKLSKA ALIEAQGILA DIREEVIEIQ KLRSKLTQGN NMQLLLEREE
SVLRYSNRYY ELVPRAVSKW EPAQPLNHEH NISQEQRLVE NLLDFEAAIK IIMGSYTKLQ
VMHPLDYCFN SLSIRMKQIQ NEHPEYKMIQ QYVSRTNHKA TDPNSQFIRN VFAVERRGEA
ERIKQFSDHT KKLLWHGSGV QNLLSILNFG LRINGIHAQK SGSSLGDGIY FADLFSKASA
YANNADVGVE SRFLLLCEVA VGKEQQIKTN ENFTKFANSN YQLMKGFNSV KLVGKSCPDE
KKNLVLPNGT IVPIGPIIDF NENLKQKLQI EKSTKKSAKK STKKPAQKIP ARPIKKVVKA
TKYSSDEENS QDEESQIQVE EDELDIPEDE IQSDGDEEIE EEEDNFDEEQ DEQNLLQNNF
LYDVQHQGAK TPFNQLFTQY QNSEYVVYNS AQVRIRYLIE IRD
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