ID I7MJP1_TETTS Unreviewed; 3282 AA.
AC I7MJP1;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 2.
DT 24-JAN-2024, entry version 48.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=TTHERM_00842700 {ECO:0000313|EMBL:EAS07044.2};
OS Tetrahymena thermophila (strain SB210).
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=312017 {ECO:0000313|EMBL:EAS07044.2, ECO:0000313|Proteomes:UP000009168};
RN [1] {ECO:0000313|Proteomes:UP000009168}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB210 {ECO:0000313|Proteomes:UP000009168};
RX PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA Hamilton E.P., Orias E.;
RT "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT model eukaryote.";
RL PLoS Biol. 4:1620-1642(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; GG662249; EAS07044.2; -; Genomic_DNA.
DR RefSeq; XP_001027286.2; XM_001027286.2.
DR STRING; 312017.I7MJP1; -.
DR GeneID; 7842144; -.
DR KEGG; tet:TTHERM_00842700; -.
DR eggNOG; KOG0939; Eukaryota.
DR InParanoid; I7MJP1; -.
DR OrthoDB; 5480520at2759; -.
DR Proteomes; UP000009168; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Reference proteome {ECO:0000313|Proteomes:UP000009168};
KW Transferase {ECO:0000313|EMBL:EAS07044.2};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 2954..3282
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1856..1922
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2095..2119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2218..2241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1634..1701
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 1980..2007
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1856..1899
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2098..2112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3249
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3282 AA; 389047 MW; 051E4F2777E6EB3E CRC64;
MEIEQSHCLL FARPFWLHNQ GDDQLMKEYC QLNEFFDQNL KKIIVSLKKI KEIQISSCEN
CVKENGHERN TQTIQQNEQS QQADQAQSMQ NQLVKEFNLE DVQMQISQLE QELLETLLSV
EKQRKYEIQK FYDGKRHSNH FWNYFVRMGG IFQHLQYLNS LEVIYRIYKL ANMFQTEILQ
ENVISSYFDN NNISYNIFAR FYHFFMTIHS VHEEYLDKIA FTDDMTIRIN IKRSQIQEIV
EKQENKYLSC YGLTKLILSS QNGINLSYSD YNHSFLRLLL AVIYLRTKQN KKIWRQLRAV
CVWRHRDNVA KHKKFKVSFT GEQAVEASIP TEYFNYLRQI KEEGFENAQN LEIEECLSQH
QAHWPDENDF PKIKGSFFEL IDNYVSNLNH IYPSFLIRIM TLVEHGINQE IYARSQKIEQ
DLQIGQNLQV KLSKLIQNMT DNLNDESTYY NTQIITYELP ELPDKVNLYQ DILQNLCIKL
IFEKQFYRKS LGWYLFYKDA NKRSKQQQIM EIIDKIYAQK ELYYIGVQIY SDFVYNFESC
YSIQTINKII EMIEYCSLKK YLISRNFNFK CLEILVERFA LDKQMKLRLA YLINRCILVV
NQKIKDKANI NCLIESRAPL FIQDLFDFLD QISISPDVNQ IYQDFAKRWV LSMTSQLLLQ
ISNNRSAITE DNVRGILEKF SNYFTDKRNN EFINALNEFM PKELLHRNII KVAYQMIKEK
EKDCLSKRQF LYCLIKYCIP MQKILEEDFL NELKNDASII SERYLLKSLV VICQKFQFEH
ITSSIYPLLL QNKSIDLNLL LRNVRNSSEM NQIFLNIKHA SILKSKYINK KEIIKDIILL
QPNNLKGEVL LLLQPMTSSF LQNLQSAIRQ ILKDHQGLAT KHSQIANMIN KTKNYELNLK
KCEKSITILK HQQDMVQQLI ELLLSQEHAI NDVISFIILY TELLYKDTKT RQAFYVVFIK
QCLPYLKYNL GEKYKQFPSF NVNNCPLIKQ IETLEEEKSD IQPQSLLQIK CNSLDYFMQL
IENLGQHSNI NLKMNYFSSF EELVECEING KEIDSKQIKY LSRYLISMRE SVSYSNIFQA
FEIIHQSVQQ KQVETQTPLF VHDIGLCILT LALKIFMKQQ NKSKNDLNDE FPEIQKIFKV
IEIILEICPS ERRFYSMIYY ILESQFQFET NFIKANIELR FKNLFYEANS FVFNSFTILE
FSKKLAEVDL YQYPIEYFNV LVSMFTYNQK DLTIKLSENI LPEFQFSDYT GSQVGLFEEI
LEKFIKTKSD FYLCFIAEIC RKYPTYLKNL VQWRYTDEKD IFDFILENQI NFNYNLGVFL
NLLCNQNYSF FMLENGLIQG TTGNVIPALI GRTLDFISKC QLNSEKDSFT LQGALNVLKY
LKPKFTYLDH MKTIITFIDF VSKQKTLQIL TLLERLGILS DIQASLKIVS ASELGDYKHA
KYFFFRNIQK FLTEENRMKS IQFTKELKEK LQYCRTQDNY NLPILSNITG SPKNQKDEPL
NIPQNIKNGL KLRYFTDKMN NKIQITYSRE DLSTYEQDLI KYNLDFSQSD EYYIIQFISQ
VKKNQRAFDG IDGDVNFKRV VLEALDIQGL SENEKKFETI ITQIFKLSLK QKTSQPASNT
KLSDRQIPDS SSLAQELDEK FKTVKKIKER LEQESAANQK EAQEKIDEQQ NGLLEDIEEQ
QKKLEQQKQE LIKQYDQLLQ DSLKQQHVIK KLSADFTNHK KPQRRQFLFD SYNFPNNSLT
LYQIDEETFY NASHNEKIRL LNEGKQTLHQ KNLLKKLKIA VQREQYSLKK FISKMQNEKL
AEKLDLQSQF DANFESFMSI CNEHEKQAIL LTFDQRYLKQ LPEKWRNEAN KIRKQHMNKG
EEIKLQESEE EEEKNSKEEE KDFPSYLTRI KRNVEDIPKR NSNSQESQQN KRQQNEKEDN
SVIQDKLFSR HEDIEYDEEQ DDNLEDNQIQ DGFEDRICLE VDEGIQQIHK SLRSSEMKQR
QKTSIEVEQI SSEIASLKQA SLEMQKQFEA AVFDGSPHLF ANLKNTNISV FSSQMDTQNQ
IEKNSNNLEG SIKNLGLVQS QDSNQKEQKI EQSQISIEKI IPQQVSSLEQ KINDNEQQAK
NQQEEKIKNN DSNEKNQLSQ EEQLLQMQNE KAMQELQIQQ QKLLIQKQIE EQERMARQIY
EQEQEIVNNQ KDDYYDYEDF DAINQQLILD QISKEREERE LKKQQLLLQQ LEEQKKQVED
GDKIQQNSSE NSSAESNLTE EEKYLQKNFS QSLPYSQEKE KKYSSIDFYL KSLYVDSNFF
LNILRLLINR GLDEYQEYQN NYLEILYILS FNPFNNYKVV QILKGMTVGI IQNQIQDKNT
QYKVKTHYLK QYFKYESDEK KANNQQCFNV FQLTQSILDY EIFNWYYFVL YVNKENQFEE
TFKWNYSILE KNIKYDESLI KLAIRKEDEH SDIMAMNSWD FSLINLLEKN IHSIIETQHV
IEKSDDYSVS ITQLRQNYDA TFNLILKFLG KFNISTNLYR KITSKLGGYT QSQQQNQQAD
NNLEKTTATI SAETYPEMKF QINVSNSFFE AYYTYSFHLK GSRKTSLKKM NNILYDLLRK
KMIENYESFE NMSLQILKIN REVINPQIIS CNQFVKNYRK LAQEQNQLKN LNGTEEIAQS
KRYIFHNTME KNYENMFFQL GTLRRTIKNL IEIFNEMFEV QCKLDLEKEY ELRKSEDGQT
KANEWLDQQK KELRKELLQK LQQVWMSQLK DLYVYLYEIL YQSIQLMSEK LDSSKFTYDR
FNLSVKSVVV LYDFFYSDNM KLNVEEDAEM QSSTHIHQFK EPLVTKHSSK EADSALNSEI
DQDQNKSLSD HLNKQFSQVL NTDLEQINLS DLYFIIEQKF IGLIVLFIET SERKKKWLEF
MKKVGHLKIR ENKNLSMEFK MSYLKIVCEI EANKKKKTKH YGFDYYGDFQ PEIRLNINRD
EIWMSTIAEL SKQDSASLVH QQLIINFEGE AGIDQGGMAR EWINLALKDV LDPRKGLFAL
SSNKITLQPN PLSCIIPDYL LHFRFAGRLV GLALTNKIDF EIDLTRSFLK HILRKQLCVS
DYEDIDPKTA KNLLWMLEND VTGSDLTYTI TQDILGIRVE KDLIKNGNKI EINNQNKVQF
VKDYLHYKMT SEIQQQLESF ISGFYDVVHP SALVFFDERD LGLRLAGSKK IDIEDMKKNT
KYSGNYDQDS HQIKWLWEVL EQFDEEMKQS FIFFLTGAFK VPFGGFEQNP IEVYDKLGNT
NSLPIAHTCF NKLELPAYQS KNQLKEKLTQ AILEGHKGFH IS
//
